Experiment: L-Alanine (C) 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_5589 and Pf1N1B4_5590 overlap by 4 nucleotides Pf1N1B4_5590 and Pf1N1B4_5591 are separated by 143 nucleotides  
        
        Pf1N1B4_5589 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 10,545 to 11,786 
         
        _5589 
         
        
        Pf1N1B4_5590 - Trans-feruloyl-CoA synthase (EC 6.2.1.34), at 11,783 to 13,666 
         
        _5590 
         
        
        Pf1N1B4_5591 - Vanillin dehydrogenase (Hydroxybenzaldehyde dehydrogenase) (EC 1.2.1.28), at 13,810 to 15,258 
         
        _5591 
         Position (kb)  
11 
 
12 
 
13 
 
14 Strain fitness (log2 ratio)  
-1 
 
0 
 
1 
 
2 at 11.111 kb on + strand, within Pf1N1B4_5589 at 11.112 kb on - strand, within Pf1N1B4_5589 at 11.295 kb on + strand, within Pf1N1B4_5589 at 11.295 kb on + strand, within Pf1N1B4_5589 at 11.314 kb on + strand, within Pf1N1B4_5589 at 11.383 kb on + strand, within Pf1N1B4_5589 at 11.383 kb on + strand, within Pf1N1B4_5589 at 11.541 kb on - strand, within Pf1N1B4_5589 at 11.542 kb on + strand, within Pf1N1B4_5589 at 11.543 kb on - strand, within Pf1N1B4_5589 at 11.543 kb on - strand, within Pf1N1B4_5589 at 11.650 kb on - strand, within Pf1N1B4_5589 at 11.740 kb on + strand at 11.741 kb on - strand at 12.154 kb on - strand, within Pf1N1B4_5590 at 12.310 kb on - strand, within Pf1N1B4_5590 at 12.373 kb on + strand, within Pf1N1B4_5590 at 12.808 kb on - strand, within Pf1N1B4_5590 at 13.288 kb on - strand, within Pf1N1B4_5590 at 13.419 kb on + strand, within Pf1N1B4_5590 at 13.559 kb on + strand at 13.560 kb on - strand at 13.560 kb on - strand at 13.604 kb on + strand at 13.737 kb on + strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.744 kb on - strand at 13.965 kb on + strand, within Pf1N1B4_5591 at 14.184 kb on + strand, within Pf1N1B4_5591  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   L-Alanine (C)             remove   11,111   +    Pf1N1B4_5589   0.46  +0.5   11,112   -    Pf1N1B4_5589   0.46  -1.3   11,295   +    Pf1N1B4_5589   0.60  -0.5   11,295   +    Pf1N1B4_5589   0.60  +0.4   11,314   +    Pf1N1B4_5589   0.62  -0.0   11,383   +    Pf1N1B4_5589   0.67  -0.9   11,383   +    Pf1N1B4_5589   0.67  -0.5   11,541   -    Pf1N1B4_5589   0.80  -0.2   11,542   +    Pf1N1B4_5589   0.80  +1.1   11,543   -    Pf1N1B4_5589   0.80  -1.5   11,543   -    Pf1N1B4_5589   0.80  -1.5   11,650   -    Pf1N1B4_5589   0.89  -0.9   11,740   +        -0.0   11,741   -        +1.1   12,154   -    Pf1N1B4_5590   0.20  +0.1   12,310   -    Pf1N1B4_5590   0.28  +2.1   12,373   +    Pf1N1B4_5590   0.31  +1.7   12,808   -    Pf1N1B4_5590   0.54  +0.3   13,288   -    Pf1N1B4_5590   0.80  +1.0   13,419   +    Pf1N1B4_5590   0.87  -0.0   13,559   +        +0.2   13,560   -        -1.0   13,560   -        -1.0   13,604   +        -0.5   13,737   +        -0.9   13,744   -        +0.5   13,744   -        -0.5   13,744   -        -0.5   13,744   -        +1.5   13,744   -        -1.5   13,965   +    Pf1N1B4_5591   0.11  -0.0   14,184   +    Pf1N1B4_5591   0.26  -1.5 
 
Or see this region's nucleotide sequence