Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5504 and Pf1N1B4_5505 are separated by 38 nucleotides Pf1N1B4_5505 and Pf1N1B4_5506 overlap by 10 nucleotides Pf1N1B4_5506 and Pf1N1B4_5507 are separated by 48 nucleotides
Pf1N1B4_5504 - hypothetical protein, at 254,478 to 255,089
_5504
Pf1N1B4_5505 - hypothetical protein, at 255,128 to 255,304
_5505
Pf1N1B4_5506 - hypothetical protein, at 255,295 to 255,819
_5506
Pf1N1B4_5507 - hypothetical protein, at 255,868 to 256,542
_5507
Position (kb)
255
256
257 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 254.893 kb on + strand, within Pf1N1B4_5504 at 254.894 kb on - strand, within Pf1N1B4_5504 at 254.894 kb on - strand, within Pf1N1B4_5504 at 255.054 kb on + strand at 255.055 kb on - strand at 255.066 kb on + strand at 255.067 kb on - strand at 255.067 kb on - strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.243 kb on - strand, within Pf1N1B4_5505 at 255.243 kb on - strand at 255.269 kb on + strand at 255.269 kb on + strand at 255.269 kb on + strand, within Pf1N1B4_5505 at 255.269 kb on + strand, within Pf1N1B4_5505 at 255.270 kb on - strand, within Pf1N1B4_5505 at 255.270 kb on - strand, within Pf1N1B4_5505 at 255.289 kb on + strand at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.593 kb on - strand, within Pf1N1B4_5506 at 255.593 kb on - strand, within Pf1N1B4_5506 at 255.593 kb on - strand at 255.593 kb on - strand at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.691 kb on + strand at 255.692 kb on - strand, within Pf1N1B4_5506 at 255.740 kb on + strand, within Pf1N1B4_5506 at 255.741 kb on - strand, within Pf1N1B4_5506 at 255.741 kb on - strand, within Pf1N1B4_5506 at 255.829 kb on + strand at 255.829 kb on + strand at 255.829 kb on + strand at 255.830 kb on - strand at 255.881 kb on + strand at 255.881 kb on + strand at 255.881 kb on + strand at 255.882 kb on - strand at 255.932 kb on + strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 256.353 kb on - strand, within Pf1N1B4_5507 at 256.405 kb on + strand, within Pf1N1B4_5507 at 256.405 kb on + strand, within Pf1N1B4_5507 at 256.406 kb on - strand, within Pf1N1B4_5507 at 256.490 kb on + strand at 256.491 kb on - strand at 256.491 kb on - strand at 256.662 kb on + strand at 256.662 kb on + strand at 256.680 kb on - strand at 256.720 kb on + strand at 256.720 kb on + strand at 256.721 kb on - strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.852 kb on - strand at 256.852 kb on - strand at 256.852 kb on - strand at 256.852 kb on - strand at 256.852 kb on - strand at 256.956 kb on + strand at 256.956 kb on + strand at 256.956 kb on + strand at 256.956 kb on + strand at 256.990 kb on + strand at 256.990 kb on + strand at 256.990 kb on + strand at 256.991 kb on - strand at 257.022 kb on + strand at 257.048 kb on + strand at 257.048 kb on + strand at 257.094 kb on + strand at 257.095 kb on - strand at 257.095 kb on - strand at 257.095 kb on - strand at 257.134 kb on + strand at 257.134 kb on + strand at 257.134 kb on + strand at 257.134 kb on + strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.156 kb on - strand at 257.177 kb on - strand at 257.186 kb on - strand at 257.231 kb on - strand at 257.304 kb on - strand at 257.372 kb on + strand at 257.372 kb on + strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.389 kb on - strand at 257.389 kb on - strand at 257.389 kb on - strand at 257.421 kb on + strand at 257.422 kb on - strand at 257.422 kb on - strand at 257.466 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 254,893 + Pf1N1B4_5504 0.68 -1.0 254,894 - Pf1N1B4_5504 0.68 +2.0 254,894 - Pf1N1B4_5504 0.68 -1.0 255,054 + +1.4 255,055 - -2.3 255,066 + -2.5 255,067 - +0.6 255,067 - -0.4 255,131 + +2.0 255,131 + +0.0 255,131 + -0.9 255,131 + -0.1 255,131 + -0.7 255,131 + -2.3 255,131 + +1.0 255,132 - +2.0 255,132 - -0.7 255,132 - -0.7 255,132 - +0.0 255,132 - -2.3 255,132 - +0.0 255,132 - -0.3 255,242 + Pf1N1B4_5505 0.64 -0.5 255,242 + Pf1N1B4_5505 0.64 -0.5 255,242 + -1.0 255,242 + Pf1N1B4_5505 0.64 -1.5 255,242 + Pf1N1B4_5505 0.64 -2.5 255,242 + +2.4 255,242 + Pf1N1B4_5505 0.64 +1.0 255,242 + Pf1N1B4_5505 0.64 +0.0 255,242 + Pf1N1B4_5505 0.64 -0.5 255,243 - Pf1N1B4_5505 0.65 -2.1 255,243 - +1.0 255,269 + +0.0 255,269 + +0.8 255,269 + Pf1N1B4_5505 0.80 -0.7 255,269 + Pf1N1B4_5505 0.80 +0.0 255,270 - Pf1N1B4_5505 0.80 +0.0 255,270 - Pf1N1B4_5505 0.80 +0.5 255,289 + -1.5 255,592 + Pf1N1B4_5506 0.57 -0.4 255,592 + Pf1N1B4_5506 0.57 +1.4 255,592 + Pf1N1B4_5506 0.57 -2.0 255,592 + Pf1N1B4_5506 0.57 +0.0 255,592 + +0.6 255,592 + Pf1N1B4_5506 0.57 -1.5 255,593 - Pf1N1B4_5506 0.57 +0.6 255,593 - Pf1N1B4_5506 0.57 -2.3 255,593 - +1.0 255,593 - +0.0 255,689 + Pf1N1B4_5506 0.75 +1.0 255,689 + Pf1N1B4_5506 0.75 -0.5 255,689 + Pf1N1B4_5506 0.75 +1.6 255,689 + Pf1N1B4_5506 0.75 -2.3 255,691 + -2.3 255,692 - Pf1N1B4_5506 0.76 -0.5 255,740 + Pf1N1B4_5506 0.85 -2.0 255,741 - Pf1N1B4_5506 0.85 +0.7 255,741 - Pf1N1B4_5506 0.85 -2.4 255,829 + -2.3 255,829 + +0.0 255,829 + -2.3 255,830 - -1.0 255,881 + +1.0 255,881 + +0.0 255,881 + +0.0 255,882 - +0.0 255,932 + +2.0 255,933 - +0.3 255,933 - -1.0 255,933 - +0.3 255,933 - -2.3 255,933 - -0.3 255,933 - +0.4 256,353 - Pf1N1B4_5507 0.72 -1.1 256,405 + Pf1N1B4_5507 0.80 -0.4 256,405 + Pf1N1B4_5507 0.80 -1.5 256,406 - Pf1N1B4_5507 0.80 -2.5 256,490 + +0.0 256,491 - -0.5 256,491 - -0.5 256,662 + +0.5 256,662 + +0.5 256,680 - +0.8 256,720 + -1.3 256,720 + +1.0 256,721 - -0.4 256,851 + +0.0 256,851 + +0.0 256,851 + -1.0 256,851 + +0.6 256,851 + +0.4 256,851 + +0.8 256,851 + +0.6 256,851 + -2.0 256,851 + +1.6 256,851 + +1.4 256,851 + +1.0 256,852 - +1.0 256,852 - +1.0 256,852 - +0.8 256,852 - +0.3 256,852 - -1.0 256,956 + -0.5 256,956 + +1.0 256,956 + +0.0 256,956 + -0.6 256,990 + -0.5 256,990 + -0.5 256,990 + -1.5 256,991 - -0.7 257,022 + -3.1 257,048 + -0.5 257,048 + +1.0 257,094 + +0.6 257,095 - -0.5 257,095 - -2.3 257,095 - +0.5 257,134 + +0.7 257,134 + +1.0 257,134 + +0.6 257,134 + -1.0 257,135 - +1.0 257,135 - +0.0 257,135 - +0.0 257,135 - -2.0 257,135 - +0.4 257,135 - -0.8 257,135 - -0.5 257,156 - +1.2 257,177 - -1.0 257,186 - -0.7 257,231 - -1.0 257,304 - -1.0 257,372 + +0.0 257,372 + +0.6 257,373 - -1.0 257,373 - -2.3 257,373 - +0.0 257,373 - +0.0 257,373 - +0.5 257,373 - -0.4 257,373 - +0.0 257,389 - +0.4 257,389 - -0.7 257,389 - +0.6 257,421 + +0.5 257,422 - -0.5 257,422 - +0.0 257,466 + -1.0
Or see this region's nucleotide sequence