Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4251 and Pf1N1B4_4252 are separated by 138 nucleotides Pf1N1B4_4252 and Pf1N1B4_4253 overlap by 8 nucleotides Pf1N1B4_4253 and Pf1N1B4_4254 are separated by 448 nucleotides Pf1N1B4_4254 and Pf1N1B4_4255 are separated by 75 nucleotides
Pf1N1B4_4251 - Outer membrane receptor proteins, likely involved in siderophore uptake @ TonB-dependent siderophore receptor, at 4,713,227 to 4,715,590
_4251
Pf1N1B4_4252 - hypothetical protein, at 4,715,729 to 4,716,265
_4252
Pf1N1B4_4253 - RNA polymerase sigma-70 factor, ECF subfamily, at 4,716,258 to 4,716,791
_4253
Pf1N1B4_4254 - Sensory box histidine kinase/response regulator, at 4,717,240 to 4,717,617
_4254
Pf1N1B4_4255 - Putative phosphatase YfbT, at 4,717,693 to 4,718,361
_4255
Position (kb)
4716
4717 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4715.282 kb on + strand, within Pf1N1B4_4251 at 4715.494 kb on - strand at 4715.494 kb on - strand at 4715.618 kb on + strand at 4715.754 kb on + strand at 4715.763 kb on + strand at 4715.764 kb on - strand at 4715.881 kb on + strand, within Pf1N1B4_4252 at 4715.881 kb on + strand, within Pf1N1B4_4252 at 4715.915 kb on + strand, within Pf1N1B4_4252 at 4716.331 kb on + strand, within Pf1N1B4_4253 at 4716.382 kb on + strand, within Pf1N1B4_4253 at 4716.450 kb on + strand, within Pf1N1B4_4253 at 4716.613 kb on + strand, within Pf1N1B4_4253 at 4716.614 kb on - strand, within Pf1N1B4_4253 at 4717.075 kb on + strand at 4717.115 kb on - strand at 4717.149 kb on + strand at 4717.150 kb on - strand at 4717.150 kb on - strand at 4717.150 kb on - strand at 4717.155 kb on + strand at 4717.156 kb on - strand at 4717.651 kb on + strand at 4717.651 kb on + strand at 4717.651 kb on + strand at 4717.651 kb on + strand at 4717.652 kb on - strand at 4717.652 kb on - strand at 4717.652 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 4,715,282 + Pf1N1B4_4251 0.87 +0.2 4,715,494 - +0.4 4,715,494 - +0.5 4,715,618 + +0.1 4,715,754 + +0.5 4,715,763 + +0.9 4,715,764 - -2.0 4,715,881 + Pf1N1B4_4252 0.28 -0.1 4,715,881 + Pf1N1B4_4252 0.28 -1.0 4,715,915 + Pf1N1B4_4252 0.35 -0.7 4,716,331 + Pf1N1B4_4253 0.14 -0.5 4,716,382 + Pf1N1B4_4253 0.23 -1.2 4,716,450 + Pf1N1B4_4253 0.36 -0.9 4,716,613 + Pf1N1B4_4253 0.66 -2.0 4,716,614 - Pf1N1B4_4253 0.67 -0.5 4,717,075 + -1.8 4,717,115 - -0.5 4,717,149 + -1.5 4,717,150 - -0.7 4,717,150 - -0.5 4,717,150 - -2.0 4,717,155 + +0.5 4,717,156 - +0.5 4,717,651 + +0.5 4,717,651 + -1.0 4,717,651 + +2.1 4,717,651 + -0.5 4,717,652 - -0.0 4,717,652 - +0.3 4,717,652 - -0.5
Or see this region's nucleotide sequence