Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4062 and Pf1N1B4_4063 overlap by 1 nucleotides Pf1N1B4_4063 and Pf1N1B4_4064 are separated by 15 nucleotides Pf1N1B4_4064 and Pf1N1B4_4065 are separated by 0 nucleotides
Pf1N1B4_4062 - FIG065221: Holliday junction DNA helicase, at 4,516,297 to 4,517,619
_4062
Pf1N1B4_4063 - CrcB protein, at 4,517,619 to 4,517,993
_4063
Pf1N1B4_4064 - Seryl-tRNA synthetase (EC 6.1.1.11), at 4,518,009 to 4,519,289
_4064
Pf1N1B4_4065 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 4,519,290 to 4,520,684
_4065
Position (kb)
4518
4519
4520 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4517.041 kb on + strand, within Pf1N1B4_4062 at 4517.042 kb on - strand, within Pf1N1B4_4062 at 4517.042 kb on - strand, within Pf1N1B4_4062 at 4517.065 kb on - strand, within Pf1N1B4_4062 at 4517.143 kb on + strand, within Pf1N1B4_4062 at 4517.143 kb on + strand, within Pf1N1B4_4062 at 4517.144 kb on - strand, within Pf1N1B4_4062 at 4517.287 kb on - strand, within Pf1N1B4_4062 at 4517.290 kb on + strand, within Pf1N1B4_4062 at 4517.291 kb on - strand, within Pf1N1B4_4062 at 4517.291 kb on - strand, within Pf1N1B4_4062 at 4517.323 kb on + strand, within Pf1N1B4_4062 at 4517.324 kb on - strand, within Pf1N1B4_4062 at 4517.324 kb on - strand, within Pf1N1B4_4062 at 4517.488 kb on + strand at 4517.489 kb on - strand at 4517.489 kb on - strand at 4517.489 kb on - strand at 4517.594 kb on + strand at 4517.595 kb on - strand at 4517.721 kb on + strand, within Pf1N1B4_4063 at 4517.721 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.941 kb on + strand, within Pf1N1B4_4063 at 4519.287 kb on + strand at 4519.296 kb on + strand at 4519.296 kb on + strand at 4519.296 kb on + strand at 4519.297 kb on - strand at 4519.333 kb on + strand at 4519.333 kb on + strand at 4519.408 kb on + strand at 4519.408 kb on + strand at 4519.741 kb on - strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.029 kb on - strand, within Pf1N1B4_4065 at 4520.029 kb on - strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.204 kb on + strand, within Pf1N1B4_4065 at 4520.205 kb on - strand, within Pf1N1B4_4065
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 4,517,041 + Pf1N1B4_4062 0.56 +0.2 4,517,042 - Pf1N1B4_4062 0.56 -0.9 4,517,042 - Pf1N1B4_4062 0.56 -0.1 4,517,065 - Pf1N1B4_4062 0.58 -0.1 4,517,143 + Pf1N1B4_4062 0.64 -3.2 4,517,143 + Pf1N1B4_4062 0.64 +0.1 4,517,144 - Pf1N1B4_4062 0.64 -1.6 4,517,287 - Pf1N1B4_4062 0.75 -1.4 4,517,290 + Pf1N1B4_4062 0.75 -0.4 4,517,291 - Pf1N1B4_4062 0.75 +0.2 4,517,291 - Pf1N1B4_4062 0.75 -0.4 4,517,323 + Pf1N1B4_4062 0.78 -0.4 4,517,324 - Pf1N1B4_4062 0.78 +0.0 4,517,324 - Pf1N1B4_4062 0.78 -0.4 4,517,488 + -1.2 4,517,489 - -1.1 4,517,489 - +0.2 4,517,489 - +0.5 4,517,594 + -0.9 4,517,595 - +0.6 4,517,721 + Pf1N1B4_4063 0.27 -0.7 4,517,721 + Pf1N1B4_4063 0.27 -1.4 4,517,773 + Pf1N1B4_4063 0.41 +0.6 4,517,773 + Pf1N1B4_4063 0.41 +0.5 4,517,773 + Pf1N1B4_4063 0.41 +0.1 4,517,773 + Pf1N1B4_4063 0.41 -0.8 4,517,773 + Pf1N1B4_4063 0.41 +0.6 4,517,773 + Pf1N1B4_4063 0.41 -0.9 4,517,941 + Pf1N1B4_4063 0.86 +0.5 4,519,287 + +0.0 4,519,296 + -2.7 4,519,296 + -3.7 4,519,296 + -0.7 4,519,297 - -1.4 4,519,333 + -2.9 4,519,333 + -2.2 4,519,408 + -2.4 4,519,408 + -1.4 4,519,741 - Pf1N1B4_4065 0.32 -0.4 4,519,834 + Pf1N1B4_4065 0.39 -1.9 4,519,834 + Pf1N1B4_4065 0.39 -0.4 4,519,834 + -0.4 4,519,834 + Pf1N1B4_4065 0.39 -1.4 4,519,834 + Pf1N1B4_4065 0.39 -1.9 4,519,834 + Pf1N1B4_4065 0.39 -1.4 4,519,834 + Pf1N1B4_4065 0.39 -2.9 4,519,835 - Pf1N1B4_4065 0.39 -2.4 4,519,835 - Pf1N1B4_4065 0.39 -0.9 4,519,835 - Pf1N1B4_4065 0.39 -1.4 4,519,835 - Pf1N1B4_4065 0.39 -1.4 4,519,835 - Pf1N1B4_4065 0.39 -2.7 4,520,028 + Pf1N1B4_4065 0.53 +0.2 4,520,028 + Pf1N1B4_4065 0.53 -1.8 4,520,028 + Pf1N1B4_4065 0.53 -2.7 4,520,028 + Pf1N1B4_4065 0.53 -1.9 4,520,028 + Pf1N1B4_4065 0.53 -1.4 4,520,028 + Pf1N1B4_4065 0.53 -1.9 4,520,029 - Pf1N1B4_4065 0.53 -2.4 4,520,029 - Pf1N1B4_4065 0.53 -2.4 4,520,088 + Pf1N1B4_4065 0.57 -2.7 4,520,088 + Pf1N1B4_4065 0.57 -2.9 4,520,088 + Pf1N1B4_4065 0.57 -3.2 4,520,088 + Pf1N1B4_4065 0.57 -0.4 4,520,088 + Pf1N1B4_4065 0.57 -2.4 4,520,204 + Pf1N1B4_4065 0.66 -4.4 4,520,205 - Pf1N1B4_4065 0.66 -1.4
Or see this region's nucleotide sequence