Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_254 and Pf1N1B4_255 are separated by 0 nucleotides Pf1N1B4_255 and Pf1N1B4_256 overlap by 4 nucleotides Pf1N1B4_256 and Pf1N1B4_257 are separated by 111 nucleotides
Pf1N1B4_254 - Adenylate cyclase (EC 4.6.1.1), at 312,051 to 314,996
_254
Pf1N1B4_255 - Free methionine-(R)-sulfoxide reductase, contains GAF domain, at 314,997 to 315,479
_255
Pf1N1B4_256 - putative ATP/GTP-binding protein, at 315,476 to 316,366
_256
Pf1N1B4_257 - sensor histidine kinase/response regulator, at 316,478 to 318,841
_257
Position (kb) 314
315
316 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 314.046 kb on + strand, within Pf1N1B4_254 at 314.145 kb on + strand, within Pf1N1B4_254 at 314.145 kb on + strand, within Pf1N1B4_254 at 314.145 kb on + strand, within Pf1N1B4_254 at 314.145 kb on + strand, within Pf1N1B4_254 at 314.146 kb on - strand, within Pf1N1B4_254 at 314.146 kb on - strand, within Pf1N1B4_254 at 314.146 kb on - strand, within Pf1N1B4_254 at 314.146 kb on - strand, within Pf1N1B4_254 at 314.146 kb on - strand, within Pf1N1B4_254 at 314.194 kb on + strand, within Pf1N1B4_254 at 314.195 kb on - strand, within Pf1N1B4_254 at 314.195 kb on - strand, within Pf1N1B4_254 at 314.509 kb on - strand, within Pf1N1B4_254 at 314.514 kb on + strand, within Pf1N1B4_254 at 314.514 kb on + strand, within Pf1N1B4_254 at 314.514 kb on + strand, within Pf1N1B4_254 at 314.514 kb on + strand, within Pf1N1B4_254 at 314.514 kb on + strand at 314.515 kb on - strand, within Pf1N1B4_254 at 314.515 kb on - strand, within Pf1N1B4_254 at 314.515 kb on - strand, within Pf1N1B4_254 at 314.515 kb on - strand at 314.532 kb on + strand, within Pf1N1B4_254 at 314.532 kb on + strand, within Pf1N1B4_254 at 314.533 kb on - strand, within Pf1N1B4_254 at 314.533 kb on - strand, within Pf1N1B4_254 at 314.578 kb on - strand, within Pf1N1B4_254 at 314.578 kb on - strand at 314.578 kb on - strand, within Pf1N1B4_254 at 314.578 kb on - strand, within Pf1N1B4_254 at 314.608 kb on + strand at 314.648 kb on + strand, within Pf1N1B4_254 at 314.648 kb on + strand, within Pf1N1B4_254 at 314.796 kb on + strand at 314.796 kb on + strand at 314.796 kb on + strand at 314.797 kb on - strand at 314.904 kb on + strand at 314.904 kb on + strand at 314.905 kb on - strand at 314.914 kb on - strand at 315.186 kb on + strand, within Pf1N1B4_255 at 315.186 kb on + strand, within Pf1N1B4_255 at 315.186 kb on + strand, within Pf1N1B4_255 at 315.186 kb on + strand, within Pf1N1B4_255 at 315.186 kb on + strand at 315.186 kb on + strand, within Pf1N1B4_255 at 315.187 kb on - strand, within Pf1N1B4_255 at 315.187 kb on - strand, within Pf1N1B4_255 at 315.187 kb on - strand, within Pf1N1B4_255 at 315.187 kb on - strand, within Pf1N1B4_255 at 315.187 kb on - strand, within Pf1N1B4_255 at 315.187 kb on - strand, within Pf1N1B4_255 at 315.187 kb on - strand at 315.187 kb on - strand, within Pf1N1B4_255 at 315.387 kb on - strand, within Pf1N1B4_255 at 315.468 kb on + strand at 315.469 kb on - strand at 315.529 kb on - strand at 315.685 kb on - strand, within Pf1N1B4_256 at 315.685 kb on - strand, within Pf1N1B4_256 at 315.685 kb on - strand, within Pf1N1B4_256 at 315.832 kb on - strand, within Pf1N1B4_256 at 315.832 kb on - strand, within Pf1N1B4_256 at 315.832 kb on - strand, within Pf1N1B4_256 at 315.844 kb on + strand, within Pf1N1B4_256 at 315.844 kb on + strand, within Pf1N1B4_256 at 316.392 kb on + strand at 316.392 kb on + strand at 316.392 kb on + strand at 316.393 kb on - strand at 316.393 kb on - strand at 316.393 kb on - strand at 316.393 kb on - strand at 316.393 kb on - strand at 316.393 kb on - strand at 316.427 kb on - strand at 316.434 kb on + strand at 316.434 kb on + strand at 316.435 kb on - strand at 316.435 kb on - strand at 316.477 kb on + strand at 316.477 kb on + strand at 316.477 kb on + strand at 316.477 kb on + strand at 316.478 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 314,046 + Pf1N1B4_254 0.68 +0.1 314,145 + Pf1N1B4_254 0.71 -0.2 314,145 + Pf1N1B4_254 0.71 +0.2 314,145 + Pf1N1B4_254 0.71 -0.3 314,145 + Pf1N1B4_254 0.71 -0.2 314,146 - Pf1N1B4_254 0.71 +0.4 314,146 - Pf1N1B4_254 0.71 +1.5 314,146 - Pf1N1B4_254 0.71 -0.6 314,146 - Pf1N1B4_254 0.71 +1.4 314,146 - Pf1N1B4_254 0.71 +0.4 314,194 + Pf1N1B4_254 0.73 +0.6 314,195 - Pf1N1B4_254 0.73 -0.2 314,195 - Pf1N1B4_254 0.73 -0.6 314,509 - Pf1N1B4_254 0.83 +0.0 314,514 + Pf1N1B4_254 0.84 +0.1 314,514 + Pf1N1B4_254 0.84 -2.5 314,514 + Pf1N1B4_254 0.84 -0.6 314,514 + Pf1N1B4_254 0.84 +0.8 314,514 + +0.2 314,515 - Pf1N1B4_254 0.84 +2.2 314,515 - Pf1N1B4_254 0.84 -0.6 314,515 - Pf1N1B4_254 0.84 -1.2 314,515 - -0.2 314,532 + Pf1N1B4_254 0.84 -1.2 314,532 + Pf1N1B4_254 0.84 -2.5 314,533 - Pf1N1B4_254 0.84 -0.2 314,533 - Pf1N1B4_254 0.84 -0.7 314,578 - Pf1N1B4_254 0.86 -0.6 314,578 - +2.4 314,578 - Pf1N1B4_254 0.86 -1.5 314,578 - Pf1N1B4_254 0.86 -0.7 314,608 + -0.2 314,648 + Pf1N1B4_254 0.88 +1.1 314,648 + Pf1N1B4_254 0.88 +0.2 314,796 + -0.6 314,796 + -1.2 314,796 + -0.2 314,797 - -0.2 314,904 + +2.4 314,904 + -1.2 314,905 - +0.0 314,914 - +0.1 315,186 + Pf1N1B4_255 0.39 -1.2 315,186 + Pf1N1B4_255 0.39 +0.8 315,186 + Pf1N1B4_255 0.39 -0.7 315,186 + Pf1N1B4_255 0.39 -1.6 315,186 + -0.2 315,186 + Pf1N1B4_255 0.39 -1.8 315,187 - Pf1N1B4_255 0.39 -1.1 315,187 - Pf1N1B4_255 0.39 -1.8 315,187 - Pf1N1B4_255 0.39 -0.2 315,187 - Pf1N1B4_255 0.39 -0.9 315,187 - Pf1N1B4_255 0.39 -0.4 315,187 - Pf1N1B4_255 0.39 -1.8 315,187 - -0.2 315,187 - Pf1N1B4_255 0.39 -0.0 315,387 - Pf1N1B4_255 0.81 -0.8 315,468 + +0.6 315,469 - -0.2 315,529 - +0.4 315,685 - Pf1N1B4_256 0.23 -1.8 315,685 - Pf1N1B4_256 0.23 -0.8 315,685 - Pf1N1B4_256 0.23 -0.0 315,832 - Pf1N1B4_256 0.40 +1.2 315,832 - Pf1N1B4_256 0.40 +0.6 315,832 - Pf1N1B4_256 0.40 -0.6 315,844 + Pf1N1B4_256 0.41 -0.6 315,844 + Pf1N1B4_256 0.41 -0.1 316,392 + +0.0 316,392 + -0.6 316,392 + +0.2 316,393 - -0.2 316,393 - +0.8 316,393 - +1.4 316,393 - -2.2 316,393 - -3.0 316,393 - -1.2 316,427 - -0.2 316,434 + +1.1 316,434 + +0.2 316,435 - +0.8 316,435 - +0.8 316,477 + -0.2 316,477 + -0.2 316,477 + +0.8 316,477 + -1.2 316,478 - -2.5
Or see this region's nucleotide sequence