Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2422 and Pf1N1B4_2423 are separated by 437 nucleotides Pf1N1B4_2423 and Pf1N1B4_2424 are separated by 443 nucleotides Pf1N1B4_2424 and Pf1N1B4_2425 overlap by 27 nucleotides
Pf1N1B4_2422 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 2,747,220 to 2,748,170
_2422
Pf1N1B4_2423 - L-serine dehydratase (EC 4.3.1.17), at 2,748,608 to 2,749,984
_2423
Pf1N1B4_2424 - hypothetical protein, at 2,750,428 to 2,750,544
_2424
Pf1N1B4_2425 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 2,750,518 to 2,751,621
_2425
Position (kb)
2748
2749
2750 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2747.737 kb on + strand, within Pf1N1B4_2422 at 2747.737 kb on + strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.802 kb on - strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.830 kb on + strand, within Pf1N1B4_2422 at 2747.831 kb on - strand, within Pf1N1B4_2422 at 2747.849 kb on + strand, within Pf1N1B4_2422 at 2747.922 kb on - strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.966 kb on - strand, within Pf1N1B4_2422 at 2747.966 kb on - strand, within Pf1N1B4_2422 at 2748.004 kb on + strand, within Pf1N1B4_2422 at 2748.005 kb on - strand, within Pf1N1B4_2422 at 2748.170 kb on + strand at 2748.226 kb on + strand at 2748.226 kb on + strand at 2748.226 kb on + strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.227 kb on - strand at 2748.300 kb on + strand at 2748.300 kb on + strand at 2748.300 kb on + strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.301 kb on - strand at 2748.322 kb on - strand at 2748.379 kb on + strand at 2748.380 kb on - strand at 2748.380 kb on - strand at 2748.380 kb on - strand at 2748.547 kb on - strand at 2748.758 kb on + strand, within Pf1N1B4_2423 at 2748.758 kb on + strand, within Pf1N1B4_2423 at 2748.759 kb on - strand, within Pf1N1B4_2423 at 2748.759 kb on - strand, within Pf1N1B4_2423 at 2748.759 kb on - strand, within Pf1N1B4_2423 at 2748.897 kb on + strand, within Pf1N1B4_2423 at 2748.898 kb on - strand, within Pf1N1B4_2423 at 2748.898 kb on - strand, within Pf1N1B4_2423 at 2748.898 kb on - strand, within Pf1N1B4_2423 at 2749.034 kb on + strand, within Pf1N1B4_2423 at 2749.034 kb on + strand, within Pf1N1B4_2423 at 2749.034 kb on + strand, within Pf1N1B4_2423 at 2749.034 kb on + strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.035 kb on - strand, within Pf1N1B4_2423 at 2749.037 kb on + strand, within Pf1N1B4_2423 at 2749.037 kb on + strand, within Pf1N1B4_2423 at 2749.037 kb on + strand, within Pf1N1B4_2423 at 2749.038 kb on - strand, within Pf1N1B4_2423 at 2749.038 kb on - strand, within Pf1N1B4_2423 at 2749.038 kb on - strand, within Pf1N1B4_2423 at 2749.118 kb on + strand, within Pf1N1B4_2423 at 2749.514 kb on + strand, within Pf1N1B4_2423 at 2749.514 kb on + strand, within Pf1N1B4_2423 at 2749.514 kb on + strand, within Pf1N1B4_2423 at 2749.515 kb on - strand, within Pf1N1B4_2423 at 2749.562 kb on + strand, within Pf1N1B4_2423 at 2749.562 kb on + strand, within Pf1N1B4_2423 at 2749.563 kb on - strand, within Pf1N1B4_2423 at 2749.744 kb on + strand, within Pf1N1B4_2423 at 2749.744 kb on + strand, within Pf1N1B4_2423 at 2749.745 kb on - strand, within Pf1N1B4_2423 at 2749.745 kb on - strand, within Pf1N1B4_2423 at 2749.745 kb on - strand at 2749.873 kb on + strand at 2749.873 kb on + strand at 2749.873 kb on + strand at 2749.873 kb on + strand at 2749.874 kb on - strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.934 kb on + strand at 2749.935 kb on - strand at 2749.935 kb on - strand at 2749.935 kb on - strand at 2749.935 kb on - strand at 2749.936 kb on + strand at 2749.936 kb on + strand at 2749.937 kb on - strand at 2749.937 kb on - strand at 2749.993 kb on + strand at 2749.994 kb on - strand at 2750.055 kb on + strand at 2750.056 kb on - strand at 2750.056 kb on - strand at 2750.146 kb on - strand at 2750.146 kb on - strand at 2750.163 kb on + strand at 2750.163 kb on + strand at 2750.163 kb on + strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.164 kb on - strand at 2750.325 kb on + strand at 2750.325 kb on + strand at 2750.325 kb on + strand at 2750.326 kb on - strand at 2750.326 kb on - strand at 2750.326 kb on - strand at 2750.326 kb on - strand at 2750.326 kb on - strand at 2750.335 kb on + strand at 2750.354 kb on + strand at 2750.354 kb on + strand at 2750.355 kb on - strand at 2750.355 kb on - strand at 2750.385 kb on - strand at 2750.405 kb on + strand at 2750.430 kb on - strand at 2750.430 kb on - strand at 2750.465 kb on - strand, within Pf1N1B4_2424 at 2750.511 kb on + strand, within Pf1N1B4_2424 at 2750.601 kb on + strand at 2750.601 kb on + strand at 2750.601 kb on + strand at 2750.602 kb on - strand at 2750.621 kb on + strand at 2750.621 kb on + strand at 2750.621 kb on + strand at 2750.622 kb on - strand at 2750.622 kb on - strand at 2750.802 kb on + strand, within Pf1N1B4_2425 at 2750.803 kb on - strand, within Pf1N1B4_2425 at 2750.803 kb on - strand, within Pf1N1B4_2425
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 2,747,737 + Pf1N1B4_2422 0.54 +0.3 2,747,737 + Pf1N1B4_2422 0.54 +1.6 2,747,738 - Pf1N1B4_2422 0.54 -0.4 2,747,738 - Pf1N1B4_2422 0.54 -0.6 2,747,738 - Pf1N1B4_2422 0.54 +1.9 2,747,802 - Pf1N1B4_2422 0.61 +0.3 2,747,803 + Pf1N1B4_2422 0.61 -0.7 2,747,803 + Pf1N1B4_2422 0.61 -1.3 2,747,803 + Pf1N1B4_2422 0.61 +1.3 2,747,803 + Pf1N1B4_2422 0.61 -0.1 2,747,803 + Pf1N1B4_2422 0.61 -0.7 2,747,803 + Pf1N1B4_2422 0.61 +0.9 2,747,803 + Pf1N1B4_2422 0.61 +0.6 2,747,804 - Pf1N1B4_2422 0.61 -1.7 2,747,804 - Pf1N1B4_2422 0.61 -0.0 2,747,804 - Pf1N1B4_2422 0.61 +0.3 2,747,804 - Pf1N1B4_2422 0.61 +0.9 2,747,804 - Pf1N1B4_2422 0.61 -0.9 2,747,804 - Pf1N1B4_2422 0.61 +0.9 2,747,830 + Pf1N1B4_2422 0.64 -1.0 2,747,831 - Pf1N1B4_2422 0.64 -2.5 2,747,849 + Pf1N1B4_2422 0.66 +0.5 2,747,922 - Pf1N1B4_2422 0.74 +0.5 2,747,965 + Pf1N1B4_2422 0.78 +1.9 2,747,965 + Pf1N1B4_2422 0.78 +1.9 2,747,965 + Pf1N1B4_2422 0.78 +0.3 2,747,966 - Pf1N1B4_2422 0.78 +1.3 2,747,966 - Pf1N1B4_2422 0.78 +0.2 2,748,004 + Pf1N1B4_2422 0.82 +1.3 2,748,005 - Pf1N1B4_2422 0.83 -1.3 2,748,170 + +1.7 2,748,226 + +0.3 2,748,226 + -0.2 2,748,226 + -0.3 2,748,227 - -0.3 2,748,227 - -0.5 2,748,227 - -0.3 2,748,227 - +0.0 2,748,227 - -1.2 2,748,227 - -1.7 2,748,227 - +0.2 2,748,227 - +0.0 2,748,227 - -0.3 2,748,300 + -0.9 2,748,300 + -0.7 2,748,300 + -0.1 2,748,301 - +0.0 2,748,301 - +1.6 2,748,301 - +0.1 2,748,301 - -1.7 2,748,301 - -1.0 2,748,301 - +0.1 2,748,301 - -0.2 2,748,301 - +0.1 2,748,301 - +0.3 2,748,301 - +0.1 2,748,301 - +1.3 2,748,301 - +0.7 2,748,301 - +0.0 2,748,322 - -1.0 2,748,379 + -0.1 2,748,380 - -0.7 2,748,380 - +0.6 2,748,380 - +0.6 2,748,547 - -0.7 2,748,758 + Pf1N1B4_2423 0.11 +0.9 2,748,758 + Pf1N1B4_2423 0.11 +1.9 2,748,759 - Pf1N1B4_2423 0.11 -0.9 2,748,759 - Pf1N1B4_2423 0.11 -0.7 2,748,759 - Pf1N1B4_2423 0.11 -1.0 2,748,897 + Pf1N1B4_2423 0.21 +0.3 2,748,898 - Pf1N1B4_2423 0.21 -0.1 2,748,898 - Pf1N1B4_2423 0.21 -1.3 2,748,898 - Pf1N1B4_2423 0.21 -0.7 2,749,034 + Pf1N1B4_2423 0.31 -1.7 2,749,034 + Pf1N1B4_2423 0.31 -0.3 2,749,034 + Pf1N1B4_2423 0.31 -0.7 2,749,034 + Pf1N1B4_2423 0.31 -0.4 2,749,035 - Pf1N1B4_2423 0.31 -0.5 2,749,035 - Pf1N1B4_2423 0.31 -2.3 2,749,035 - Pf1N1B4_2423 0.31 +0.3 2,749,035 - Pf1N1B4_2423 0.31 +0.3 2,749,035 - Pf1N1B4_2423 0.31 +1.1 2,749,035 - Pf1N1B4_2423 0.31 +1.1 2,749,035 - Pf1N1B4_2423 0.31 +0.6 2,749,035 - Pf1N1B4_2423 0.31 -0.3 2,749,035 - Pf1N1B4_2423 0.31 +1.1 2,749,037 + Pf1N1B4_2423 0.31 -1.3 2,749,037 + Pf1N1B4_2423 0.31 -0.5 2,749,037 + Pf1N1B4_2423 0.31 -1.7 2,749,038 - Pf1N1B4_2423 0.31 -0.8 2,749,038 - Pf1N1B4_2423 0.31 -1.3 2,749,038 - Pf1N1B4_2423 0.31 -0.5 2,749,118 + Pf1N1B4_2423 0.37 -0.7 2,749,514 + Pf1N1B4_2423 0.66 +0.3 2,749,514 + Pf1N1B4_2423 0.66 -1.1 2,749,514 + Pf1N1B4_2423 0.66 +0.3 2,749,515 - Pf1N1B4_2423 0.66 -0.7 2,749,562 + Pf1N1B4_2423 0.69 -0.1 2,749,562 + Pf1N1B4_2423 0.69 -0.6 2,749,563 - Pf1N1B4_2423 0.69 -0.1 2,749,744 + Pf1N1B4_2423 0.82 -0.1 2,749,744 + Pf1N1B4_2423 0.82 -0.5 2,749,745 - Pf1N1B4_2423 0.83 +0.7 2,749,745 - Pf1N1B4_2423 0.83 +0.1 2,749,745 - -1.7 2,749,873 + +0.1 2,749,873 + -1.3 2,749,873 + -0.5 2,749,873 + -0.4 2,749,874 - -0.7 2,749,934 + -0.3 2,749,934 + -1.0 2,749,934 + -1.3 2,749,934 + -2.8 2,749,934 + +1.9 2,749,934 + +0.3 2,749,935 - +0.3 2,749,935 - -0.7 2,749,935 - -0.3 2,749,935 - +1.3 2,749,936 + -0.1 2,749,936 + -1.3 2,749,937 - +1.9 2,749,937 - +1.9 2,749,993 + -1.3 2,749,994 - -0.3 2,750,055 + -0.7 2,750,056 - -1.3 2,750,056 - +0.3 2,750,146 - +0.3 2,750,146 - -1.5 2,750,163 + +1.3 2,750,163 + +0.3 2,750,163 + -0.3 2,750,164 - +0.2 2,750,164 - -1.3 2,750,164 - +0.3 2,750,164 - -0.8 2,750,164 - -1.3 2,750,164 - -0.7 2,750,325 + -1.0 2,750,325 + +1.1 2,750,325 + +2.3 2,750,326 - -0.4 2,750,326 - -0.3 2,750,326 - -0.7 2,750,326 - +0.3 2,750,326 - +1.3 2,750,335 + +0.1 2,750,354 + -1.7 2,750,354 + +0.3 2,750,355 - -0.3 2,750,355 - +0.3 2,750,385 - +0.0 2,750,405 + -1.4 2,750,430 - -0.9 2,750,430 - +0.1 2,750,465 - Pf1N1B4_2424 0.32 -0.7 2,750,511 + Pf1N1B4_2424 0.71 -1.0 2,750,601 + -0.3 2,750,601 + +1.6 2,750,601 + -0.4 2,750,602 - +1.3 2,750,621 + +2.1 2,750,621 + -0.7 2,750,621 + -0.7 2,750,622 - +0.5 2,750,622 - -0.3 2,750,802 + Pf1N1B4_2425 0.26 +0.3 2,750,803 - Pf1N1B4_2425 0.26 -0.7 2,750,803 - Pf1N1B4_2425 0.26 +0.3
Or see this region's nucleotide sequence