Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2420 and Pf1N1B4_2421 overlap by 4 nucleotides Pf1N1B4_2421 and Pf1N1B4_2422 are separated by 77 nucleotides
Pf1N1B4_2420 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 2,745,122 to 2,746,300
_2420
Pf1N1B4_2421 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 2,746,297 to 2,747,142
_2421
Pf1N1B4_2422 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 2,747,220 to 2,748,170
_2422
Position (kb)
2746
2747
2748 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2745.473 kb on + strand, within Pf1N1B4_2420 at 2745.473 kb on + strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.513 kb on + strand, within Pf1N1B4_2420 at 2745.514 kb on - strand, within Pf1N1B4_2420 at 2745.514 kb on - strand, within Pf1N1B4_2420 at 2745.555 kb on + strand, within Pf1N1B4_2420 at 2746.131 kb on - strand, within Pf1N1B4_2420 at 2746.312 kb on + strand at 2746.312 kb on + strand at 2746.313 kb on - strand at 2746.339 kb on - strand at 2746.364 kb on - strand at 2746.626 kb on - strand, within Pf1N1B4_2421 at 2746.626 kb on - strand, within Pf1N1B4_2421 at 2746.710 kb on + strand, within Pf1N1B4_2421 at 2746.775 kb on + strand, within Pf1N1B4_2421 at 2746.775 kb on + strand, within Pf1N1B4_2421 at 2746.776 kb on - strand, within Pf1N1B4_2421 at 2746.776 kb on - strand at 2746.776 kb on - strand, within Pf1N1B4_2421 at 2746.776 kb on - strand, within Pf1N1B4_2421 at 2746.969 kb on + strand, within Pf1N1B4_2421 at 2747.108 kb on + strand at 2747.109 kb on - strand at 2747.109 kb on - strand at 2747.109 kb on - strand at 2747.122 kb on + strand at 2747.123 kb on - strand at 2747.170 kb on + strand at 2747.170 kb on + strand at 2747.170 kb on + strand at 2747.265 kb on - strand at 2747.473 kb on + strand at 2747.473 kb on + strand, within Pf1N1B4_2422 at 2747.474 kb on - strand, within Pf1N1B4_2422 at 2747.474 kb on - strand, within Pf1N1B4_2422 at 2747.474 kb on - strand, within Pf1N1B4_2422 at 2747.474 kb on - strand, within Pf1N1B4_2422 at 2747.506 kb on + strand, within Pf1N1B4_2422 at 2747.507 kb on - strand, within Pf1N1B4_2422 at 2747.507 kb on - strand, within Pf1N1B4_2422 at 2747.527 kb on + strand, within Pf1N1B4_2422 at 2747.527 kb on + strand, within Pf1N1B4_2422 at 2747.527 kb on + strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.737 kb on + strand, within Pf1N1B4_2422 at 2747.737 kb on + strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.802 kb on - strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.830 kb on + strand, within Pf1N1B4_2422 at 2747.831 kb on - strand, within Pf1N1B4_2422 at 2747.849 kb on + strand, within Pf1N1B4_2422 at 2747.922 kb on - strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.966 kb on - strand, within Pf1N1B4_2422 at 2747.966 kb on - strand, within Pf1N1B4_2422 at 2748.004 kb on + strand, within Pf1N1B4_2422 at 2748.005 kb on - strand, within Pf1N1B4_2422
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 2,745,473 + Pf1N1B4_2420 0.30 +0.3 2,745,473 + Pf1N1B4_2420 0.30 -0.6 2,745,474 - Pf1N1B4_2420 0.30 +0.1 2,745,474 - Pf1N1B4_2420 0.30 -1.0 2,745,474 - Pf1N1B4_2420 0.30 -2.1 2,745,474 - Pf1N1B4_2420 0.30 +1.3 2,745,474 - Pf1N1B4_2420 0.30 +1.1 2,745,474 - Pf1N1B4_2420 0.30 -0.2 2,745,474 - Pf1N1B4_2420 0.30 +0.3 2,745,474 - Pf1N1B4_2420 0.30 -2.3 2,745,513 + Pf1N1B4_2420 0.33 +0.3 2,745,514 - Pf1N1B4_2420 0.33 +0.3 2,745,514 - Pf1N1B4_2420 0.33 -0.0 2,745,555 + Pf1N1B4_2420 0.37 +0.1 2,746,131 - Pf1N1B4_2420 0.86 -1.4 2,746,312 + +1.1 2,746,312 + +2.1 2,746,313 - -1.0 2,746,339 - -0.4 2,746,364 - -0.4 2,746,626 - Pf1N1B4_2421 0.39 -0.7 2,746,626 - Pf1N1B4_2421 0.39 -1.5 2,746,710 + Pf1N1B4_2421 0.49 -0.9 2,746,775 + Pf1N1B4_2421 0.57 -1.8 2,746,775 + Pf1N1B4_2421 0.57 +1.3 2,746,776 - Pf1N1B4_2421 0.57 -0.7 2,746,776 - +0.3 2,746,776 - Pf1N1B4_2421 0.57 -1.3 2,746,776 - Pf1N1B4_2421 0.57 +1.3 2,746,969 + Pf1N1B4_2421 0.79 -0.7 2,747,108 + -2.7 2,747,109 - +0.3 2,747,109 - +0.3 2,747,109 - -0.4 2,747,122 + -0.6 2,747,123 - -0.3 2,747,170 + -0.1 2,747,170 + -1.5 2,747,170 + +2.9 2,747,265 - +0.9 2,747,473 + +0.7 2,747,473 + Pf1N1B4_2422 0.27 +0.0 2,747,474 - Pf1N1B4_2422 0.27 +1.2 2,747,474 - Pf1N1B4_2422 0.27 -0.1 2,747,474 - Pf1N1B4_2422 0.27 +0.3 2,747,474 - Pf1N1B4_2422 0.27 -2.0 2,747,506 + Pf1N1B4_2422 0.30 +1.9 2,747,507 - Pf1N1B4_2422 0.30 +1.5 2,747,507 - Pf1N1B4_2422 0.30 -0.1 2,747,527 + Pf1N1B4_2422 0.32 +0.0 2,747,527 + Pf1N1B4_2422 0.32 +0.3 2,747,527 + Pf1N1B4_2422 0.32 -1.3 2,747,528 - Pf1N1B4_2422 0.32 +0.3 2,747,528 - Pf1N1B4_2422 0.32 -0.4 2,747,528 - Pf1N1B4_2422 0.32 -0.9 2,747,528 - Pf1N1B4_2422 0.32 -0.8 2,747,528 - Pf1N1B4_2422 0.32 -1.3 2,747,737 + Pf1N1B4_2422 0.54 +0.3 2,747,737 + Pf1N1B4_2422 0.54 +1.6 2,747,738 - Pf1N1B4_2422 0.54 -0.4 2,747,738 - Pf1N1B4_2422 0.54 -0.6 2,747,738 - Pf1N1B4_2422 0.54 +1.9 2,747,802 - Pf1N1B4_2422 0.61 +0.3 2,747,803 + Pf1N1B4_2422 0.61 -0.7 2,747,803 + Pf1N1B4_2422 0.61 -1.3 2,747,803 + Pf1N1B4_2422 0.61 +1.3 2,747,803 + Pf1N1B4_2422 0.61 -0.1 2,747,803 + Pf1N1B4_2422 0.61 -0.7 2,747,803 + Pf1N1B4_2422 0.61 +0.9 2,747,803 + Pf1N1B4_2422 0.61 +0.6 2,747,804 - Pf1N1B4_2422 0.61 -1.7 2,747,804 - Pf1N1B4_2422 0.61 -0.0 2,747,804 - Pf1N1B4_2422 0.61 +0.3 2,747,804 - Pf1N1B4_2422 0.61 +0.9 2,747,804 - Pf1N1B4_2422 0.61 -0.9 2,747,804 - Pf1N1B4_2422 0.61 +0.9 2,747,830 + Pf1N1B4_2422 0.64 -1.0 2,747,831 - Pf1N1B4_2422 0.64 -2.5 2,747,849 + Pf1N1B4_2422 0.66 +0.5 2,747,922 - Pf1N1B4_2422 0.74 +0.5 2,747,965 + Pf1N1B4_2422 0.78 +1.9 2,747,965 + Pf1N1B4_2422 0.78 +1.9 2,747,965 + Pf1N1B4_2422 0.78 +0.3 2,747,966 - Pf1N1B4_2422 0.78 +1.3 2,747,966 - Pf1N1B4_2422 0.78 +0.2 2,748,004 + Pf1N1B4_2422 0.82 +1.3 2,748,005 - Pf1N1B4_2422 0.83 -1.3
Or see this region's nucleotide sequence