Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2328 and Pf1N1B4_2329 are separated by 74 nucleotides Pf1N1B4_2329 and Pf1N1B4_2330 are separated by 72 nucleotides Pf1N1B4_2330 and Pf1N1B4_2331 are separated by 60 nucleotides
Pf1N1B4_2328 - Monofunctional biosynthetic peptidoglycan transglycosylase (EC 2.4.2.-), at 2,648,347 to 2,649,069
_2328
Pf1N1B4_2329 - COG2363, at 2,649,144 to 2,649,515
_2329
Pf1N1B4_2330 - Sulfur carrier protein ThiS, at 2,649,588 to 2,649,806
_2330
Pf1N1B4_2331 - Thiazole biosynthesis protein ThiG, at 2,649,867 to 2,650,661
_2331
Position (kb)
2649
2650 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2648.286 kb on - strand at 2648.324 kb on - strand at 2648.324 kb on - strand at 2648.393 kb on + strand at 2648.393 kb on + strand at 2648.393 kb on + strand at 2648.393 kb on + strand at 2648.393 kb on + strand at 2648.393 kb on + strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.397 kb on + strand at 2648.397 kb on + strand at 2648.398 kb on - strand at 2648.407 kb on + strand at 2648.407 kb on + strand at 2648.407 kb on + strand at 2648.408 kb on - strand at 2648.408 kb on - strand at 2648.408 kb on - strand at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.510 kb on - strand, within Pf1N1B4_2328 at 2648.615 kb on + strand, within Pf1N1B4_2328 at 2648.615 kb on + strand, within Pf1N1B4_2328 at 2648.616 kb on - strand, within Pf1N1B4_2328 at 2648.616 kb on - strand, within Pf1N1B4_2328 at 2648.616 kb on - strand, within Pf1N1B4_2328 at 2648.616 kb on - strand, within Pf1N1B4_2328 at 2648.616 kb on - strand, within Pf1N1B4_2328 at 2648.616 kb on - strand, within Pf1N1B4_2328 at 2649.007 kb on + strand at 2649.007 kb on + strand at 2649.058 kb on + strand at 2649.069 kb on + strand at 2649.069 kb on + strand at 2649.070 kb on - strand at 2649.070 kb on - strand at 2649.070 kb on - strand at 2649.105 kb on + strand at 2649.105 kb on + strand at 2649.106 kb on - strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.276 kb on + strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.290 kb on + strand, within Pf1N1B4_2329 at 2649.310 kb on + strand, within Pf1N1B4_2329 at 2649.311 kb on - strand, within Pf1N1B4_2329 at 2649.311 kb on - strand, within Pf1N1B4_2329 at 2649.402 kb on + strand, within Pf1N1B4_2329 at 2649.402 kb on + strand, within Pf1N1B4_2329 at 2649.402 kb on + strand, within Pf1N1B4_2329 at 2649.784 kb on + strand, within Pf1N1B4_2330 at 2649.852 kb on + strand at 2649.881 kb on + strand at 2649.911 kb on + strand at 2649.911 kb on + strand at 2649.911 kb on + strand at 2649.912 kb on - strand at 2649.915 kb on + strand at 2649.915 kb on + strand at 2649.915 kb on + strand at 2649.915 kb on + strand at 2649.916 kb on - strand at 2649.934 kb on + strand at 2649.934 kb on + strand at 2649.935 kb on - strand at 2649.939 kb on - strand at 2650.083 kb on + strand, within Pf1N1B4_2331 at 2650.084 kb on - strand, within Pf1N1B4_2331 at 2650.084 kb on - strand, within Pf1N1B4_2331 at 2650.098 kb on - strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.285 kb on - strand, within Pf1N1B4_2331 at 2650.285 kb on - strand, within Pf1N1B4_2331
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 2,648,286 - +2.4 2,648,324 - -0.0 2,648,324 - +0.4 2,648,393 + +0.1 2,648,393 + +0.4 2,648,393 + +0.4 2,648,393 + +0.7 2,648,393 + +2.0 2,648,393 + -0.2 2,648,394 - -0.6 2,648,394 - -0.6 2,648,394 - -0.6 2,648,394 - -0.4 2,648,394 - -0.3 2,648,394 - +0.1 2,648,394 - -1.2 2,648,394 - +0.1 2,648,397 + -2.4 2,648,397 + -1.7 2,648,398 - +1.4 2,648,407 + -1.6 2,648,407 + -0.6 2,648,407 + +1.1 2,648,408 - +1.0 2,648,408 - -0.9 2,648,408 - -1.6 2,648,453 + Pf1N1B4_2328 0.15 -1.9 2,648,453 + Pf1N1B4_2328 0.15 +2.0 2,648,453 + Pf1N1B4_2328 0.15 -2.4 2,648,453 + Pf1N1B4_2328 0.15 -0.6 2,648,453 + Pf1N1B4_2328 0.15 -0.4 2,648,453 + Pf1N1B4_2328 0.15 -0.6 2,648,453 + Pf1N1B4_2328 0.15 +2.4 2,648,454 - Pf1N1B4_2328 0.15 +0.4 2,648,454 - Pf1N1B4_2328 0.15 +1.1 2,648,454 - Pf1N1B4_2328 0.15 +1.4 2,648,454 - Pf1N1B4_2328 0.15 +1.4 2,648,454 - Pf1N1B4_2328 0.15 +0.6 2,648,454 - Pf1N1B4_2328 0.15 -1.2 2,648,454 - Pf1N1B4_2328 0.15 -0.5 2,648,454 - Pf1N1B4_2328 0.15 -0.2 2,648,454 - Pf1N1B4_2328 0.15 +1.0 2,648,454 - Pf1N1B4_2328 0.15 -2.4 2,648,510 - Pf1N1B4_2328 0.23 +0.2 2,648,615 + Pf1N1B4_2328 0.37 -0.8 2,648,615 + Pf1N1B4_2328 0.37 -0.0 2,648,616 - Pf1N1B4_2328 0.37 +0.4 2,648,616 - Pf1N1B4_2328 0.37 +0.4 2,648,616 - Pf1N1B4_2328 0.37 +0.1 2,648,616 - Pf1N1B4_2328 0.37 -0.1 2,648,616 - Pf1N1B4_2328 0.37 +1.0 2,648,616 - Pf1N1B4_2328 0.37 +0.4 2,649,007 + -1.8 2,649,007 + -0.3 2,649,058 + +0.4 2,649,069 + -1.3 2,649,069 + -0.9 2,649,070 - +0.7 2,649,070 - -0.2 2,649,070 - +2.4 2,649,105 + +0.4 2,649,105 + -0.6 2,649,106 - +0.8 2,649,107 + +0.4 2,649,107 + -0.6 2,649,107 + -0.3 2,649,107 + +0.4 2,649,107 + -1.6 2,649,107 + -0.6 2,649,107 + -1.9 2,649,107 + -0.9 2,649,107 + +0.4 2,649,107 + +0.4 2,649,107 + +0.7 2,649,107 + -0.3 2,649,108 - -0.6 2,649,108 - -1.2 2,649,108 - +0.4 2,649,108 - +0.4 2,649,108 - +0.6 2,649,108 - +1.0 2,649,108 - +0.5 2,649,108 - +1.4 2,649,108 - +2.4 2,649,108 - -1.2 2,649,108 - +1.4 2,649,108 - +1.1 2,649,108 - -1.2 2,649,108 - -1.8 2,649,108 - -0.2 2,649,108 - +0.4 2,649,276 + Pf1N1B4_2329 0.35 -0.0 2,649,277 - Pf1N1B4_2329 0.36 -1.2 2,649,277 - Pf1N1B4_2329 0.36 -0.2 2,649,277 - Pf1N1B4_2329 0.36 -1.2 2,649,277 - Pf1N1B4_2329 0.36 -0.6 2,649,277 - Pf1N1B4_2329 0.36 -2.4 2,649,277 - Pf1N1B4_2329 0.36 -0.0 2,649,277 - Pf1N1B4_2329 0.36 +0.4 2,649,290 + Pf1N1B4_2329 0.39 -0.3 2,649,310 + Pf1N1B4_2329 0.45 -1.2 2,649,311 - Pf1N1B4_2329 0.45 -1.8 2,649,311 - Pf1N1B4_2329 0.45 -1.0 2,649,402 + Pf1N1B4_2329 0.69 -0.2 2,649,402 + Pf1N1B4_2329 0.69 -0.6 2,649,402 + Pf1N1B4_2329 0.69 -2.2 2,649,784 + Pf1N1B4_2330 0.89 -0.9 2,649,852 + +0.1 2,649,881 + +0.4 2,649,911 + -0.2 2,649,911 + -0.6 2,649,911 + -0.4 2,649,912 - +0.4 2,649,915 + -2.4 2,649,915 + -0.6 2,649,915 + +1.4 2,649,915 + +0.4 2,649,916 - -1.6 2,649,934 + +0.4 2,649,934 + -1.2 2,649,935 - +1.7 2,649,939 - -0.2 2,650,083 + Pf1N1B4_2331 0.27 +1.0 2,650,084 - Pf1N1B4_2331 0.27 -0.3 2,650,084 - Pf1N1B4_2331 0.27 +0.4 2,650,098 - Pf1N1B4_2331 0.29 +1.0 2,650,284 + Pf1N1B4_2331 0.52 -1.4 2,650,284 + Pf1N1B4_2331 0.52 -0.5 2,650,284 + Pf1N1B4_2331 0.52 -0.6 2,650,284 + Pf1N1B4_2331 0.52 -1.6 2,650,284 + Pf1N1B4_2331 0.52 -0.6 2,650,285 - Pf1N1B4_2331 0.53 -1.2 2,650,285 - Pf1N1B4_2331 0.53 +1.0
Or see this region's nucleotide sequence