Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2144 and Pf1N1B4_2145 overlap by 29 nucleotides Pf1N1B4_2145 and Pf1N1B4_2146 are separated by 97 nucleotides Pf1N1B4_2146 and Pf1N1B4_2147 are separated by 129 nucleotides Pf1N1B4_2147 and Pf1N1B4_2148 are separated by 317 nucleotides
Pf1N1B4_2144 - D-amino acid dehydrogenase small subunit (EC 1.4.99.1), at 2,454,539 to 2,455,843
_2144
Pf1N1B4_2145 - Translation initiation inhibitor, at 2,455,815 to 2,456,168
_2145
Pf1N1B4_2146 - Alanine racemase (EC 5.1.1.1), at 2,456,266 to 2,457,339
_2146
Pf1N1B4_2147 - Putrescine utilization regulator, at 2,457,469 to 2,458,017
_2147
Pf1N1B4_2148 - Cytochrome c5, at 2,458,335 to 2,458,613
_2148
Position (kb)
2456
2457
2458 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2455.283 kb on + strand, within Pf1N1B4_2144 at 2455.283 kb on + strand, within Pf1N1B4_2144 at 2455.305 kb on - strand, within Pf1N1B4_2144 at 2455.305 kb on - strand, within Pf1N1B4_2144 at 2455.349 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.433 kb on - strand, within Pf1N1B4_2144 at 2455.433 kb on - strand, within Pf1N1B4_2144 at 2455.433 kb on - strand, within Pf1N1B4_2144 at 2455.774 kb on + strand at 2455.774 kb on + strand at 2455.874 kb on + strand, within Pf1N1B4_2145 at 2456.013 kb on + strand, within Pf1N1B4_2145 at 2456.251 kb on + strand at 2457.325 kb on + strand at 2457.326 kb on - strand at 2457.326 kb on - strand at 2457.326 kb on - strand at 2457.398 kb on + strand at 2457.398 kb on + strand at 2457.398 kb on + strand at 2457.398 kb on + strand at 2457.399 kb on - strand at 2457.402 kb on + strand at 2457.402 kb on + strand at 2457.402 kb on + strand at 2457.403 kb on - strand at 2457.403 kb on - strand at 2457.403 kb on - strand at 2457.433 kb on + strand at 2457.434 kb on - strand at 2457.434 kb on - strand at 2457.434 kb on - strand at 2457.550 kb on - strand, within Pf1N1B4_2147 at 2457.694 kb on + strand, within Pf1N1B4_2147 at 2457.694 kb on + strand, within Pf1N1B4_2147 at 2457.695 kb on - strand, within Pf1N1B4_2147 at 2457.796 kb on + strand, within Pf1N1B4_2147 at 2457.925 kb on + strand, within Pf1N1B4_2147 at 2457.925 kb on + strand, within Pf1N1B4_2147 at 2457.925 kb on + strand at 2457.925 kb on + strand, within Pf1N1B4_2147 at 2457.925 kb on + strand, within Pf1N1B4_2147 at 2457.925 kb on + strand at 2457.925 kb on + strand, within Pf1N1B4_2147 at 2457.925 kb on + strand, within Pf1N1B4_2147 at 2457.926 kb on - strand, within Pf1N1B4_2147 at 2457.926 kb on - strand, within Pf1N1B4_2147 at 2457.928 kb on + strand, within Pf1N1B4_2147 at 2457.928 kb on + strand, within Pf1N1B4_2147 at 2457.928 kb on + strand, within Pf1N1B4_2147 at 2457.928 kb on + strand, within Pf1N1B4_2147 at 2457.928 kb on + strand, within Pf1N1B4_2147 at 2457.928 kb on + strand at 2457.929 kb on - strand, within Pf1N1B4_2147 at 2457.929 kb on - strand, within Pf1N1B4_2147 at 2458.016 kb on - strand at 2458.060 kb on + strand at 2458.060 kb on + strand at 2458.061 kb on - strand at 2458.061 kb on - strand at 2458.061 kb on - strand at 2458.061 kb on - strand at 2458.061 kb on - strand at 2458.061 kb on - strand at 2458.061 kb on - strand at 2458.119 kb on - strand at 2458.124 kb on + strand at 2458.125 kb on - strand at 2458.125 kb on - strand at 2458.125 kb on - strand at 2458.126 kb on + strand at 2458.126 kb on + strand at 2458.126 kb on + strand at 2458.126 kb on + strand at 2458.126 kb on + strand at 2458.126 kb on + strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.127 kb on - strand at 2458.166 kb on - strand at 2458.183 kb on + strand at 2458.183 kb on + strand at 2458.183 kb on + strand at 2458.183 kb on + strand at 2458.183 kb on + strand at 2458.183 kb on + strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.184 kb on - strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.195 kb on + strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.196 kb on - strand at 2458.199 kb on + strand at 2458.199 kb on + strand at 2458.199 kb on + strand at 2458.199 kb on + strand at 2458.199 kb on + strand at 2458.199 kb on + strand at 2458.200 kb on - strand at 2458.200 kb on - strand at 2458.200 kb on - strand at 2458.200 kb on - strand at 2458.200 kb on - strand at 2458.228 kb on + strand at 2458.229 kb on - strand at 2458.274 kb on + strand at 2458.274 kb on + strand at 2458.275 kb on - strand at 2458.280 kb on + strand at 2458.281 kb on - strand at 2458.281 kb on - strand at 2458.281 kb on - strand at 2458.281 kb on - strand at 2458.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 2,455,283 + Pf1N1B4_2144 0.57 -1.1 2,455,283 + Pf1N1B4_2144 0.57 -3.5 2,455,305 - Pf1N1B4_2144 0.59 -3.1 2,455,305 - Pf1N1B4_2144 0.59 -1.9 2,455,349 + Pf1N1B4_2144 0.62 -1.6 2,455,430 + Pf1N1B4_2144 0.68 +1.0 2,455,430 + Pf1N1B4_2144 0.68 +0.4 2,455,430 + Pf1N1B4_2144 0.68 -1.9 2,455,430 + Pf1N1B4_2144 0.68 -1.6 2,455,430 + Pf1N1B4_2144 0.68 -1.1 2,455,430 + Pf1N1B4_2144 0.68 -2.6 2,455,430 + Pf1N1B4_2144 0.68 -1.1 2,455,430 + Pf1N1B4_2144 0.68 -1.1 2,455,430 + Pf1N1B4_2144 0.68 -2.1 2,455,430 + Pf1N1B4_2144 0.68 -1.2 2,455,430 + Pf1N1B4_2144 0.68 -2.6 2,455,430 + Pf1N1B4_2144 0.68 -1.6 2,455,430 + Pf1N1B4_2144 0.68 -1.1 2,455,430 + Pf1N1B4_2144 0.68 -1.9 2,455,430 + Pf1N1B4_2144 0.68 -0.6 2,455,430 + Pf1N1B4_2144 0.68 -3.1 2,455,430 + Pf1N1B4_2144 0.68 -1.1 2,455,430 + Pf1N1B4_2144 0.68 -2.9 2,455,430 + Pf1N1B4_2144 0.68 -1.6 2,455,430 + Pf1N1B4_2144 0.68 -1.6 2,455,430 + Pf1N1B4_2144 0.68 -0.6 2,455,430 + Pf1N1B4_2144 0.68 -2.4 2,455,430 + Pf1N1B4_2144 0.68 -2.6 2,455,430 + Pf1N1B4_2144 0.68 -2.6 2,455,431 - Pf1N1B4_2144 0.68 -1.6 2,455,431 - Pf1N1B4_2144 0.68 -3.3 2,455,431 - Pf1N1B4_2144 0.68 -1.9 2,455,431 - Pf1N1B4_2144 0.68 -2.7 2,455,431 - Pf1N1B4_2144 0.68 -1.1 2,455,431 - Pf1N1B4_2144 0.68 -1.1 2,455,432 + Pf1N1B4_2144 0.68 -1.6 2,455,432 + Pf1N1B4_2144 0.68 -0.6 2,455,432 + Pf1N1B4_2144 0.68 -1.9 2,455,432 + Pf1N1B4_2144 0.68 -1.9 2,455,432 + Pf1N1B4_2144 0.68 -4.0 2,455,433 - Pf1N1B4_2144 0.69 -1.9 2,455,433 - Pf1N1B4_2144 0.69 -2.7 2,455,433 - Pf1N1B4_2144 0.69 -1.1 2,455,774 + -3.6 2,455,774 + -1.6 2,455,874 + Pf1N1B4_2145 0.17 -0.4 2,456,013 + Pf1N1B4_2145 0.56 +0.1 2,456,251 + -0.6 2,457,325 + +0.3 2,457,326 - -1.1 2,457,326 - -0.0 2,457,326 - -0.3 2,457,398 + -0.1 2,457,398 + -1.1 2,457,398 + -0.6 2,457,398 + +0.0 2,457,399 - -1.1 2,457,402 + +0.4 2,457,402 + -0.1 2,457,402 + +1.4 2,457,403 - +0.9 2,457,403 - +0.4 2,457,403 - +1.8 2,457,433 + +1.4 2,457,434 - -1.6 2,457,434 - +1.0 2,457,434 - +0.4 2,457,550 - Pf1N1B4_2147 0.15 +0.4 2,457,694 + Pf1N1B4_2147 0.41 +1.4 2,457,694 + Pf1N1B4_2147 0.41 +0.4 2,457,695 - Pf1N1B4_2147 0.41 +0.4 2,457,796 + Pf1N1B4_2147 0.60 +0.0 2,457,925 + Pf1N1B4_2147 0.83 -0.6 2,457,925 + Pf1N1B4_2147 0.83 -1.7 2,457,925 + +0.4 2,457,925 + Pf1N1B4_2147 0.83 +0.4 2,457,925 + Pf1N1B4_2147 0.83 +1.0 2,457,925 + +1.4 2,457,925 + Pf1N1B4_2147 0.83 +0.4 2,457,925 + Pf1N1B4_2147 0.83 -0.6 2,457,926 - Pf1N1B4_2147 0.83 -0.3 2,457,926 - Pf1N1B4_2147 0.83 -1.4 2,457,928 + Pf1N1B4_2147 0.84 -0.9 2,457,928 + Pf1N1B4_2147 0.84 -1.9 2,457,928 + Pf1N1B4_2147 0.84 +0.2 2,457,928 + Pf1N1B4_2147 0.84 -1.1 2,457,928 + Pf1N1B4_2147 0.84 -0.6 2,457,928 + -1.1 2,457,929 - Pf1N1B4_2147 0.84 +0.7 2,457,929 - Pf1N1B4_2147 0.84 +0.4 2,458,016 - -1.9 2,458,060 + -0.1 2,458,060 + -0.6 2,458,061 - -1.1 2,458,061 - +0.7 2,458,061 - -0.1 2,458,061 - +0.4 2,458,061 - -0.0 2,458,061 - -0.6 2,458,061 - +0.4 2,458,119 - -0.2 2,458,124 + -0.1 2,458,125 - -0.9 2,458,125 - -0.8 2,458,125 - +0.1 2,458,126 + -1.1 2,458,126 + -0.3 2,458,126 + +0.2 2,458,126 + +0.4 2,458,126 + +3.0 2,458,126 + -2.7 2,458,127 - -0.6 2,458,127 - -0.7 2,458,127 - -0.6 2,458,127 - -0.8 2,458,127 - -0.7 2,458,127 - -0.7 2,458,127 - +0.6 2,458,127 - +0.9 2,458,127 - +0.4 2,458,127 - -0.8 2,458,127 - -0.3 2,458,127 - -0.9 2,458,127 - -1.1 2,458,127 - -0.8 2,458,127 - +2.8 2,458,127 - -0.2 2,458,127 - -0.2 2,458,127 - +0.4 2,458,127 - +0.9 2,458,127 - -0.9 2,458,127 - -0.8 2,458,127 - +0.1 2,458,127 - +2.0 2,458,127 - -1.5 2,458,127 - -0.9 2,458,127 - +0.8 2,458,127 - +1.9 2,458,127 - -2.6 2,458,127 - -0.0 2,458,127 - -0.8 2,458,127 - +0.4 2,458,166 - -0.1 2,458,183 + +0.4 2,458,183 + -1.1 2,458,183 + +0.9 2,458,183 + -0.4 2,458,183 + +0.2 2,458,183 + +1.4 2,458,184 - -1.1 2,458,184 - -0.4 2,458,184 - -1.9 2,458,184 - +0.8 2,458,184 - -0.6 2,458,184 - -2.1 2,458,184 - +0.2 2,458,184 - +0.4 2,458,184 - -0.1 2,458,184 - +0.1 2,458,195 + -1.3 2,458,195 + -0.3 2,458,195 + -0.8 2,458,195 + +1.0 2,458,195 + -1.9 2,458,195 + -1.4 2,458,195 + -0.9 2,458,195 + -0.6 2,458,195 + -1.4 2,458,195 + -0.5 2,458,195 + +0.9 2,458,195 + -1.0 2,458,195 + -0.0 2,458,195 + -0.6 2,458,195 + -0.1 2,458,195 + -1.4 2,458,195 + -0.5 2,458,195 + +0.4 2,458,195 + -0.6 2,458,195 + -0.6 2,458,195 + +2.4 2,458,195 + -1.9 2,458,195 + -1.1 2,458,195 + +0.0 2,458,195 + -0.6 2,458,195 + -1.6 2,458,195 + +0.0 2,458,195 + -0.9 2,458,195 + +0.4 2,458,195 + -0.9 2,458,195 + +1.4 2,458,195 + +1.4 2,458,195 + -0.4 2,458,195 + -1.6 2,458,195 + -0.1 2,458,196 - -0.7 2,458,196 - -1.4 2,458,196 - -0.6 2,458,196 - -0.6 2,458,196 - +0.2 2,458,196 - -0.4 2,458,196 - -0.1 2,458,196 - -0.6 2,458,196 - +0.5 2,458,196 - +0.0 2,458,196 - -0.9 2,458,196 - +0.4 2,458,196 - -0.1 2,458,196 - +1.4 2,458,196 - +0.4 2,458,196 - -1.1 2,458,196 - -0.6 2,458,196 - -0.6 2,458,196 - -0.6 2,458,196 - -0.6 2,458,196 - -0.1 2,458,196 - -0.6 2,458,196 - -1.0 2,458,196 - +0.1 2,458,196 - -0.4 2,458,196 - +1.4 2,458,196 - +0.2 2,458,196 - +0.4 2,458,196 - -0.6 2,458,199 + -0.6 2,458,199 + -0.3 2,458,199 + -0.6 2,458,199 + -0.6 2,458,199 + -0.1 2,458,199 + +0.1 2,458,200 - -0.1 2,458,200 - -1.1 2,458,200 - +0.3 2,458,200 - -0.9 2,458,200 - -0.9 2,458,228 + -1.4 2,458,229 - -1.1 2,458,274 + -1.1 2,458,274 + -0.6 2,458,275 - +0.4 2,458,280 + -0.6 2,458,281 - -1.6 2,458,281 - -0.6 2,458,281 - -0.3 2,458,281 - -1.1 2,458,281 - -1.1
Or see this region's nucleotide sequence