Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1012 and Pf1N1B4_1013 are separated by 135 nucleotides Pf1N1B4_1013 and Pf1N1B4_1014 are separated by 63 nucleotides
Pf1N1B4_1012 - Glycine cleavage system transcriptional activator, at 1,147,624 to 1,148,571
_1012
Pf1N1B4_1013 - L-serine ammonia-lyase (EC 4.3.1.17) (from data) , at 1,148,707 to 1,150,083
_1013
Pf1N1B4_1014 - Serine transporter, at 1,150,147 to 1,151,448
_1014
Position (kb)
1148
1149
1150
1151 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1147.866 kb on + strand, within Pf1N1B4_1012 at 1147.890 kb on + strand, within Pf1N1B4_1012 at 1147.891 kb on - strand, within Pf1N1B4_1012 at 1147.937 kb on + strand, within Pf1N1B4_1012 at 1147.937 kb on + strand, within Pf1N1B4_1012 at 1147.937 kb on + strand, within Pf1N1B4_1012 at 1147.938 kb on - strand, within Pf1N1B4_1012 at 1147.938 kb on - strand, within Pf1N1B4_1012 at 1147.938 kb on - strand, within Pf1N1B4_1012 at 1147.956 kb on - strand, within Pf1N1B4_1012 at 1147.961 kb on + strand, within Pf1N1B4_1012 at 1147.962 kb on - strand, within Pf1N1B4_1012 at 1148.071 kb on + strand at 1148.072 kb on - strand, within Pf1N1B4_1012 at 1148.072 kb on - strand, within Pf1N1B4_1012 at 1148.072 kb on - strand at 1148.423 kb on - strand, within Pf1N1B4_1012 at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.544 kb on + strand at 1148.544 kb on - strand at 1148.597 kb on + strand at 1148.694 kb on + strand at 1148.697 kb on - strand at 1148.857 kb on + strand, within Pf1N1B4_1013 at 1149.019 kb on - strand, within Pf1N1B4_1013 at 1149.019 kb on - strand, within Pf1N1B4_1013 at 1149.070 kb on + strand, within Pf1N1B4_1013 at 1149.070 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.136 kb on + strand, within Pf1N1B4_1013 at 1149.137 kb on - strand, within Pf1N1B4_1013 at 1149.218 kb on - strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.346 kb on - strand, within Pf1N1B4_1013 at 1149.346 kb on - strand, within Pf1N1B4_1013 at 1149.346 kb on - strand, within Pf1N1B4_1013 at 1149.530 kb on + strand, within Pf1N1B4_1013 at 1149.573 kb on + strand, within Pf1N1B4_1013 at 1149.573 kb on + strand, within Pf1N1B4_1013 at 1149.573 kb on + strand, within Pf1N1B4_1013 at 1149.574 kb on - strand, within Pf1N1B4_1013 at 1149.613 kb on + strand, within Pf1N1B4_1013 at 1149.613 kb on + strand, within Pf1N1B4_1013 at 1149.613 kb on + strand, within Pf1N1B4_1013 at 1149.613 kb on + strand, within Pf1N1B4_1013 at 1149.614 kb on - strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.616 kb on + strand, within Pf1N1B4_1013 at 1149.617 kb on - strand, within Pf1N1B4_1013 at 1149.617 kb on - strand, within Pf1N1B4_1013 at 1149.788 kb on + strand, within Pf1N1B4_1013 at 1149.788 kb on + strand, within Pf1N1B4_1013 at 1149.788 kb on + strand, within Pf1N1B4_1013 at 1149.789 kb on - strand, within Pf1N1B4_1013 at 1149.972 kb on + strand at 1149.972 kb on + strand at 1149.972 kb on + strand at 1149.972 kb on + strand at 1149.972 kb on + strand at 1149.972 kb on + strand at 1149.973 kb on - strand at 1150.033 kb on + strand at 1150.033 kb on + strand at 1150.033 kb on + strand at 1150.033 kb on + strand at 1150.033 kb on + strand at 1150.033 kb on + strand at 1150.034 kb on - strand at 1150.108 kb on + strand at 1150.108 kb on + strand at 1150.109 kb on - strand at 1150.109 kb on - strand at 1150.109 kb on - strand at 1150.109 kb on - strand at 1150.109 kb on - strand at 1150.109 kb on - strand at 1150.128 kb on + strand at 1150.128 kb on + strand at 1150.128 kb on + strand at 1150.128 kb on + strand at 1150.129 kb on - strand at 1150.129 kb on - strand at 1150.132 kb on - strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.157 kb on + strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.158 kb on - strand at 1150.161 kb on + strand at 1150.161 kb on + strand at 1150.161 kb on + strand at 1150.161 kb on + strand at 1150.161 kb on + strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.162 kb on - strand at 1150.187 kb on + strand at 1150.187 kb on + strand at 1150.187 kb on + strand at 1150.187 kb on + strand at 1150.188 kb on - strand at 1150.201 kb on - strand at 1150.201 kb on - strand at 1150.201 kb on - strand at 1150.201 kb on - strand at 1150.201 kb on - strand at 1150.201 kb on - strand at 1150.201 kb on - strand at 1150.211 kb on + strand at 1150.212 kb on - strand at 1150.261 kb on + strand at 1150.262 kb on - strand at 1150.262 kb on - strand at 1150.480 kb on + strand, within Pf1N1B4_1014 at 1150.480 kb on + strand, within Pf1N1B4_1014 at 1150.480 kb on + strand, within Pf1N1B4_1014 at 1150.481 kb on - strand, within Pf1N1B4_1014 at 1150.481 kb on - strand, within Pf1N1B4_1014 at 1150.513 kb on + strand, within Pf1N1B4_1014 at 1150.513 kb on + strand, within Pf1N1B4_1014 at 1150.513 kb on + strand, within Pf1N1B4_1014 at 1150.513 kb on + strand, within Pf1N1B4_1014 at 1150.514 kb on - strand, within Pf1N1B4_1014 at 1150.514 kb on - strand, within Pf1N1B4_1014 at 1151.029 kb on + strand, within Pf1N1B4_1014 at 1151.065 kb on + strand, within Pf1N1B4_1014 at 1151.066 kb on - strand, within Pf1N1B4_1014 at 1151.066 kb on - strand, within Pf1N1B4_1014
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 1,147,866 + Pf1N1B4_1012 0.26 +1.1 1,147,890 + Pf1N1B4_1012 0.28 +0.1 1,147,891 - Pf1N1B4_1012 0.28 +0.5 1,147,937 + Pf1N1B4_1012 0.33 -0.9 1,147,937 + Pf1N1B4_1012 0.33 +0.1 1,147,937 + Pf1N1B4_1012 0.33 -2.2 1,147,938 - Pf1N1B4_1012 0.33 +1.7 1,147,938 - Pf1N1B4_1012 0.33 +0.1 1,147,938 - Pf1N1B4_1012 0.33 -0.4 1,147,956 - Pf1N1B4_1012 0.35 -1.9 1,147,961 + Pf1N1B4_1012 0.36 +1.1 1,147,962 - Pf1N1B4_1012 0.36 +0.2 1,148,071 + +1.7 1,148,072 - Pf1N1B4_1012 0.47 -1.2 1,148,072 - Pf1N1B4_1012 0.47 -0.6 1,148,072 - +0.1 1,148,423 - Pf1N1B4_1012 0.84 +0.7 1,148,543 + -0.9 1,148,543 + +2.1 1,148,543 + -1.9 1,148,543 + -0.5 1,148,543 + +0.7 1,148,543 + +0.1 1,148,543 + -2.2 1,148,543 + -0.3 1,148,543 + -0.5 1,148,544 + -0.9 1,148,544 - -2.2 1,148,597 + -0.6 1,148,694 + -1.2 1,148,697 - -0.3 1,148,857 + Pf1N1B4_1013 0.11 +0.1 1,149,019 - Pf1N1B4_1013 0.23 +0.1 1,149,019 - Pf1N1B4_1013 0.23 +1.4 1,149,070 + Pf1N1B4_1013 0.26 -0.5 1,149,070 + Pf1N1B4_1013 0.26 +0.5 1,149,133 + Pf1N1B4_1013 0.31 -1.3 1,149,133 + Pf1N1B4_1013 0.31 -1.9 1,149,133 + Pf1N1B4_1013 0.31 +0.7 1,149,133 + Pf1N1B4_1013 0.31 +0.1 1,149,133 + Pf1N1B4_1013 0.31 +0.1 1,149,133 + Pf1N1B4_1013 0.31 -0.9 1,149,133 + Pf1N1B4_1013 0.31 -0.4 1,149,133 + Pf1N1B4_1013 0.31 -1.9 1,149,133 + Pf1N1B4_1013 0.31 +0.4 1,149,133 + Pf1N1B4_1013 0.31 -0.7 1,149,133 + Pf1N1B4_1013 0.31 -0.4 1,149,133 + Pf1N1B4_1013 0.31 +0.1 1,149,133 + Pf1N1B4_1013 0.31 +0.1 1,149,134 - Pf1N1B4_1013 0.31 -0.9 1,149,134 - Pf1N1B4_1013 0.31 -0.9 1,149,134 - Pf1N1B4_1013 0.31 -1.1 1,149,134 - Pf1N1B4_1013 0.31 -0.2 1,149,134 - Pf1N1B4_1013 0.31 -0.3 1,149,134 - Pf1N1B4_1013 0.31 -0.9 1,149,134 - Pf1N1B4_1013 0.31 -0.3 1,149,136 + Pf1N1B4_1013 0.31 -1.0 1,149,137 - Pf1N1B4_1013 0.31 +0.7 1,149,218 - Pf1N1B4_1013 0.37 -1.1 1,149,345 + Pf1N1B4_1013 0.46 -0.6 1,149,345 + Pf1N1B4_1013 0.46 -1.7 1,149,345 + Pf1N1B4_1013 0.46 -0.9 1,149,345 + Pf1N1B4_1013 0.46 -1.5 1,149,345 + Pf1N1B4_1013 0.46 +0.1 1,149,345 + Pf1N1B4_1013 0.46 -0.0 1,149,345 + Pf1N1B4_1013 0.46 +0.1 1,149,345 + Pf1N1B4_1013 0.46 +0.5 1,149,345 + Pf1N1B4_1013 0.46 -1.2 1,149,345 + Pf1N1B4_1013 0.46 -1.2 1,149,346 - Pf1N1B4_1013 0.46 -2.2 1,149,346 - Pf1N1B4_1013 0.46 -0.5 1,149,346 - Pf1N1B4_1013 0.46 -0.2 1,149,530 + Pf1N1B4_1013 0.60 -2.2 1,149,573 + Pf1N1B4_1013 0.63 -0.2 1,149,573 + Pf1N1B4_1013 0.63 -1.1 1,149,573 + Pf1N1B4_1013 0.63 -0.6 1,149,574 - Pf1N1B4_1013 0.63 -0.4 1,149,613 + Pf1N1B4_1013 0.66 -0.6 1,149,613 + Pf1N1B4_1013 0.66 -1.2 1,149,613 + Pf1N1B4_1013 0.66 +2.1 1,149,613 + Pf1N1B4_1013 0.66 -1.9 1,149,614 - Pf1N1B4_1013 0.66 -0.9 1,149,616 + Pf1N1B4_1013 0.66 -0.7 1,149,616 + Pf1N1B4_1013 0.66 +1.1 1,149,616 + Pf1N1B4_1013 0.66 +1.5 1,149,616 + Pf1N1B4_1013 0.66 -0.9 1,149,616 + Pf1N1B4_1013 0.66 -0.2 1,149,616 + Pf1N1B4_1013 0.66 -0.1 1,149,616 + Pf1N1B4_1013 0.66 +0.1 1,149,616 + Pf1N1B4_1013 0.66 +1.1 1,149,616 + Pf1N1B4_1013 0.66 +1.1 1,149,616 + Pf1N1B4_1013 0.66 +0.3 1,149,616 + Pf1N1B4_1013 0.66 +0.4 1,149,616 + Pf1N1B4_1013 0.66 -0.2 1,149,616 + Pf1N1B4_1013 0.66 +1.7 1,149,617 - Pf1N1B4_1013 0.66 -0.9 1,149,617 - Pf1N1B4_1013 0.66 +0.1 1,149,788 + Pf1N1B4_1013 0.79 -1.5 1,149,788 + Pf1N1B4_1013 0.79 +0.5 1,149,788 + Pf1N1B4_1013 0.79 +0.1 1,149,789 - Pf1N1B4_1013 0.79 -0.1 1,149,972 + +1.1 1,149,972 + -0.2 1,149,972 + +1.1 1,149,972 + -0.1 1,149,972 + -0.2 1,149,972 + -0.7 1,149,973 - +1.1 1,150,033 + -0.9 1,150,033 + +0.4 1,150,033 + +0.4 1,150,033 + -1.7 1,150,033 + -0.1 1,150,033 + -1.5 1,150,034 - -0.3 1,150,108 + +0.4 1,150,108 + +0.1 1,150,109 - -1.5 1,150,109 - -0.3 1,150,109 - -0.3 1,150,109 - +0.1 1,150,109 - -2.9 1,150,109 - +0.8 1,150,128 + -2.9 1,150,128 + -1.2 1,150,128 + +0.1 1,150,128 + -1.9 1,150,129 - -0.5 1,150,129 - +0.1 1,150,132 - -3.2 1,150,157 + +0.9 1,150,157 + -0.6 1,150,157 + -1.5 1,150,157 + +0.1 1,150,157 + +1.3 1,150,157 + +0.4 1,150,157 + +0.1 1,150,157 + -0.9 1,150,157 + -1.5 1,150,157 + -1.5 1,150,157 + +0.4 1,150,157 + -0.8 1,150,157 + +0.1 1,150,157 + -2.5 1,150,157 + +0.4 1,150,157 + -0.3 1,150,157 + -0.9 1,150,158 - -0.9 1,150,158 - +0.5 1,150,158 - -1.5 1,150,158 - -1.5 1,150,158 - -0.5 1,150,158 - +1.7 1,150,158 - -1.5 1,150,158 - -1.2 1,150,158 - -0.7 1,150,158 - -0.9 1,150,158 - -0.2 1,150,158 - +0.6 1,150,158 - -0.7 1,150,158 - +0.7 1,150,158 - -1.9 1,150,158 - -0.5 1,150,158 - +0.7 1,150,161 + -1.2 1,150,161 + -0.9 1,150,161 + +0.1 1,150,161 + +0.1 1,150,161 + +0.1 1,150,162 - -0.7 1,150,162 - -0.3 1,150,162 - -0.1 1,150,162 - +0.9 1,150,162 - +0.1 1,150,162 - +0.7 1,150,162 - -0.6 1,150,162 - +0.7 1,150,162 - +0.4 1,150,162 - -0.5 1,150,187 + +2.7 1,150,187 + +0.1 1,150,187 + -0.5 1,150,187 + -0.8 1,150,188 - -0.5 1,150,201 - +0.3 1,150,201 - -1.2 1,150,201 - -0.9 1,150,201 - -0.9 1,150,201 - +0.3 1,150,201 - -0.5 1,150,201 - -0.3 1,150,211 + -1.6 1,150,212 - +1.8 1,150,261 + -0.9 1,150,262 - -0.6 1,150,262 - -0.9 1,150,480 + Pf1N1B4_1014 0.26 +1.4 1,150,480 + Pf1N1B4_1014 0.26 -1.5 1,150,480 + Pf1N1B4_1014 0.26 +0.4 1,150,481 - Pf1N1B4_1014 0.26 -0.3 1,150,481 - Pf1N1B4_1014 0.26 -0.5 1,150,513 + Pf1N1B4_1014 0.28 -1.5 1,150,513 + Pf1N1B4_1014 0.28 -0.2 1,150,513 + Pf1N1B4_1014 0.28 +0.9 1,150,513 + Pf1N1B4_1014 0.28 -1.1 1,150,514 - Pf1N1B4_1014 0.28 +2.1 1,150,514 - Pf1N1B4_1014 0.28 -1.2 1,151,029 + Pf1N1B4_1014 0.68 -1.9 1,151,065 + Pf1N1B4_1014 0.71 -0.2 1,151,066 - Pf1N1B4_1014 0.71 -1.2 1,151,066 - Pf1N1B4_1014 0.71 +1.9
Or see this region's nucleotide sequence