Experiment: 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05087 and H281DRAFT_05088 are separated by 54 nucleotides H281DRAFT_05088 and H281DRAFT_05089 are separated by 4 nucleotides H281DRAFT_05089 and H281DRAFT_05090 overlap by 4 nucleotides H281DRAFT_05090 and H281DRAFT_05091 are separated by 550 nucleotides H281DRAFT_05091 and H281DRAFT_05092 are separated by 96 nucleotides
H281DRAFT_05087: H281DRAFT_05087 - exodeoxyribonuclease V alpha subunit, at 30,679 to 33,003
_05087
H281DRAFT_05088: H281DRAFT_05088 - hypothetical protein, at 33,058 to 33,201
_05088
H281DRAFT_05089: H281DRAFT_05089 - ribosome-associated protein, at 33,206 to 33,610
_05089
H281DRAFT_05090: H281DRAFT_05090 - EAL domain, c-di-GMP-specific phosphodiesterase class I (or its enzymatically inactive variant), at 33,607 to 34,932
_05090
H281DRAFT_05091: H281DRAFT_05091 - Quinol monooxygenase YgiN, at 35,483 to 35,773
_05091
H281DRAFT_05092: H281DRAFT_05092 - hypothetical protein, at 35,870 to 36,127
_05092
Position (kb) 33
34
35 Strain fitness (log2 ratio) -1
0
1
2
3 at 33.019 kb on - strand at 33.176 kb on - strand, within H281DRAFT_05088 at 33.176 kb on - strand, within H281DRAFT_05088 at 33.199 kb on + strand at 33.200 kb on - strand at 33.543 kb on + strand, within H281DRAFT_05089 at 33.544 kb on - strand, within H281DRAFT_05089 at 33.608 kb on + strand at 33.725 kb on + strand at 33.725 kb on + strand at 33.726 kb on - strand at 33.726 kb on - strand at 34.076 kb on + strand, within H281DRAFT_05090 at 34.501 kb on - strand, within H281DRAFT_05090 at 34.810 kb on - strand at 34.909 kb on - strand at 34.999 kb on + strand at 35.000 kb on - strand at 35.000 kb on - strand at 35.002 kb on + strand at 35.100 kb on + strand at 35.100 kb on + strand at 35.100 kb on + strand at 35.101 kb on - strand at 35.317 kb on + strand at 35.317 kb on + strand at 35.318 kb on - strand at 35.528 kb on + strand, within H281DRAFT_05091 at 35.708 kb on + strand, within H281DRAFT_05091 at 35.709 kb on - strand, within H281DRAFT_05091 at 35.771 kb on + strand at 35.771 kb on + strand at 35.771 kb on + strand at 35.772 kb on - strand at 35.871 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 2.5 mM (C) remove 33,019 - +0.5 33,176 - H281DRAFT_05088 0.82 -0.5 33,176 - H281DRAFT_05088 0.82 +0.8 33,199 + +0.0 33,200 - +0.9 33,543 + H281DRAFT_05089 0.83 -0.2 33,544 - H281DRAFT_05089 0.83 +0.9 33,608 + +0.7 33,725 + -1.0 33,725 + +0.1 33,726 - -0.3 33,726 - -0.5 34,076 + H281DRAFT_05090 0.35 +0.1 34,501 - H281DRAFT_05090 0.67 -0.9 34,810 - +0.4 34,909 - +0.7 34,999 + -0.5 35,000 - -0.3 35,000 - +0.2 35,002 + +0.7 35,100 + -0.3 35,100 + +0.1 35,100 + +0.1 35,101 - +0.1 35,317 + -0.9 35,317 + -1.0 35,318 - -1.6 35,528 + H281DRAFT_05091 0.15 -0.9 35,708 + H281DRAFT_05091 0.77 +0.1 35,709 - H281DRAFT_05091 0.78 +0.1 35,771 + -0.1 35,771 + -0.1 35,771 + -0.4 35,772 - +3.2 35,871 + -1.3
Or see this region's nucleotide sequence