Experiment: 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04249 and H281DRAFT_04250 overlap by 4 nucleotides H281DRAFT_04250 and H281DRAFT_04251 are separated by 56 nucleotides H281DRAFT_04251 and H281DRAFT_04252 are separated by 120 nucleotides H281DRAFT_04252 and H281DRAFT_04253 are separated by 128 nucleotides
H281DRAFT_04249: H281DRAFT_04249 - tRNA-U20-dihydrouridine synthase, at 369,070 to 370,146
_04249
H281DRAFT_04250: H281DRAFT_04250 - DNA-binding protein Fis, at 370,143 to 370,376
_04250
H281DRAFT_04251: H281DRAFT_04251 - IMP cyclohydrolase /phosphoribosylaminoimidazolecarboxamide formyltransferase, at 370,433 to 371,998
_04251
H281DRAFT_04252: H281DRAFT_04252 - hypothetical protein, at 372,119 to 372,358
_04252
H281DRAFT_04253: H281DRAFT_04253 - Holliday junction endonuclease RuvC, at 372,487 to 373,029
_04253
Position (kb) 370
371
372 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 369.617 kb on - strand, within H281DRAFT_04249 at 369.634 kb on + strand, within H281DRAFT_04249 at 369.696 kb on - strand, within H281DRAFT_04249 at 369.696 kb on - strand, within H281DRAFT_04249 at 369.887 kb on - strand, within H281DRAFT_04249 at 369.895 kb on + strand, within H281DRAFT_04249 at 369.896 kb on - strand, within H281DRAFT_04249 at 369.943 kb on + strand, within H281DRAFT_04249 at 369.944 kb on - strand, within H281DRAFT_04249 at 369.990 kb on + strand, within H281DRAFT_04249 at 369.991 kb on - strand, within H281DRAFT_04249 at 370.072 kb on + strand at 370.136 kb on + strand at 370.154 kb on + strand at 370.154 kb on + strand at 370.157 kb on + strand at 370.157 kb on + strand at 370.158 kb on - strand at 370.191 kb on + strand, within H281DRAFT_04250 at 370.191 kb on + strand, within H281DRAFT_04250 at 370.230 kb on + strand, within H281DRAFT_04250 at 370.230 kb on + strand, within H281DRAFT_04250 at 370.318 kb on - strand at 370.337 kb on + strand, within H281DRAFT_04250 at 370.337 kb on + strand, within H281DRAFT_04250 at 370.337 kb on + strand, within H281DRAFT_04250 at 370.374 kb on + strand at 370.374 kb on + strand at 370.374 kb on + strand at 370.384 kb on + strand at 372.039 kb on + strand at 372.049 kb on + strand at 372.050 kb on - strand at 372.137 kb on - strand at 372.177 kb on + strand, within H281DRAFT_04252 at 372.177 kb on + strand, within H281DRAFT_04252 at 372.177 kb on + strand, within H281DRAFT_04252 at 372.177 kb on + strand, within H281DRAFT_04252 at 372.178 kb on - strand, within H281DRAFT_04252 at 372.178 kb on - strand, within H281DRAFT_04252 at 372.178 kb on - strand, within H281DRAFT_04252 at 372.178 kb on - strand, within H281DRAFT_04252 at 372.255 kb on + strand, within H281DRAFT_04252 at 372.256 kb on - strand, within H281DRAFT_04252 at 372.261 kb on + strand at 372.261 kb on + strand, within H281DRAFT_04252 at 372.261 kb on + strand, within H281DRAFT_04252 at 372.261 kb on + strand, within H281DRAFT_04252 at 372.262 kb on - strand at 372.262 kb on - strand, within H281DRAFT_04252 at 372.262 kb on - strand, within H281DRAFT_04252 at 372.374 kb on + strand at 372.374 kb on + strand at 372.374 kb on + strand at 372.375 kb on - strand at 372.375 kb on - strand at 372.375 kb on - strand at 372.375 kb on - strand at 372.444 kb on + strand at 372.444 kb on + strand at 372.444 kb on + strand at 372.444 kb on + strand at 372.444 kb on + strand at 372.444 kb on + strand at 372.445 kb on - strand at 372.445 kb on - strand at 372.447 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 2.5 mM (C) remove 369,617 - H281DRAFT_04249 0.51 -0.1 369,634 + H281DRAFT_04249 0.52 +0.1 369,696 - H281DRAFT_04249 0.58 +0.2 369,696 - H281DRAFT_04249 0.58 +0.4 369,887 - H281DRAFT_04249 0.76 -0.1 369,895 + H281DRAFT_04249 0.77 +0.9 369,896 - H281DRAFT_04249 0.77 +0.6 369,943 + H281DRAFT_04249 0.81 -1.2 369,944 - H281DRAFT_04249 0.81 +1.5 369,990 + H281DRAFT_04249 0.85 -1.8 369,991 - H281DRAFT_04249 0.86 +2.7 370,072 + +0.9 370,136 + +0.2 370,154 + +0.7 370,154 + +3.4 370,157 + +0.9 370,157 + +0.8 370,158 - +3.3 370,191 + H281DRAFT_04250 0.21 +0.9 370,191 + H281DRAFT_04250 0.21 +1.7 370,230 + H281DRAFT_04250 0.37 -0.2 370,230 + H281DRAFT_04250 0.37 +0.5 370,318 - +1.5 370,337 + H281DRAFT_04250 0.83 +0.7 370,337 + H281DRAFT_04250 0.83 +1.0 370,337 + H281DRAFT_04250 0.83 +1.0 370,374 + -0.5 370,374 + -0.2 370,374 + +0.4 370,384 + +0.1 372,039 + +0.4 372,049 + -0.8 372,050 - -1.9 372,137 - -0.0 372,177 + H281DRAFT_04252 0.24 +0.5 372,177 + H281DRAFT_04252 0.24 -0.0 372,177 + H281DRAFT_04252 0.24 +0.8 372,177 + H281DRAFT_04252 0.24 -0.4 372,178 - H281DRAFT_04252 0.25 +0.3 372,178 - H281DRAFT_04252 0.25 -0.4 372,178 - H281DRAFT_04252 0.25 +0.9 372,178 - H281DRAFT_04252 0.25 -0.5 372,255 + H281DRAFT_04252 0.57 +0.5 372,256 - H281DRAFT_04252 0.57 +0.3 372,261 + +0.9 372,261 + H281DRAFT_04252 0.59 +0.1 372,261 + H281DRAFT_04252 0.59 -0.3 372,261 + H281DRAFT_04252 0.59 -0.7 372,262 - +2.5 372,262 - H281DRAFT_04252 0.60 -0.6 372,262 - H281DRAFT_04252 0.60 -0.9 372,374 + -0.6 372,374 + +0.6 372,374 + +0.5 372,375 - +0.8 372,375 - +0.5 372,375 - -0.9 372,375 - -0.1 372,444 + +1.5 372,444 + +0.0 372,444 + +0.3 372,444 + +0.1 372,444 + +0.9 372,444 + +0.1 372,445 - -0.4 372,445 - -0.7 372,447 + +0.9
Or see this region's nucleotide sequence