Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_04220

Experiment: 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_04219 and H281DRAFT_04220 are separated by 21 nucleotidesH281DRAFT_04220 and H281DRAFT_04221 are separated by 126 nucleotidesH281DRAFT_04221 and H281DRAFT_04222 are separated by 217 nucleotides H281DRAFT_04219: H281DRAFT_04219 - Cytochrome c553, at 338,217 to 338,936 _04219 H281DRAFT_04220: H281DRAFT_04220 - Cytochrome c, mono- and diheme variants, at 338,958 to 340,256 _04220 H281DRAFT_04221: H281DRAFT_04221 - hemolysin III, at 340,383 to 341,003 _04221 H281DRAFT_04222: H281DRAFT_04222 - ATP-dependent RNA helicase RhlE, at 341,221 to 342,753 _04222 Position (kb) 338 339 340 341Strain fitness (log2 ratio) -1 0 1 2 3at 338.106 kb on + strandat 338.107 kb on - strandat 338.107 kb on - strandat 338.130 kb on - strandat 338.212 kb on + strandat 338.213 kb on - strandat 338.280 kb on + strandat 338.280 kb on + strandat 338.281 kb on - strandat 338.281 kb on - strandat 338.281 kb on - strandat 338.281 kb on - strandat 338.282 kb on + strandat 338.322 kb on - strand, within H281DRAFT_04219at 338.325 kb on - strandat 338.378 kb on + strandat 338.378 kb on + strand, within H281DRAFT_04219at 338.424 kb on + strand, within H281DRAFT_04219at 338.425 kb on - strand, within H281DRAFT_04219at 338.430 kb on + strand, within H281DRAFT_04219at 338.431 kb on - strand, within H281DRAFT_04219at 338.431 kb on - strand, within H281DRAFT_04219at 338.484 kb on + strand, within H281DRAFT_04219at 338.485 kb on - strand, within H281DRAFT_04219at 338.485 kb on - strand, within H281DRAFT_04219at 338.497 kb on - strand, within H281DRAFT_04219at 338.511 kb on + strand, within H281DRAFT_04219at 338.512 kb on - strand, within H281DRAFT_04219at 338.532 kb on + strand, within H281DRAFT_04219at 338.532 kb on + strand, within H281DRAFT_04219at 338.532 kb on + strand, within H281DRAFT_04219at 338.533 kb on - strand, within H281DRAFT_04219at 338.533 kb on - strand, within H281DRAFT_04219at 338.728 kb on - strand, within H281DRAFT_04219at 339.114 kb on + strandat 339.114 kb on + strand, within H281DRAFT_04220at 339.115 kb on - strand, within H281DRAFT_04220at 339.138 kb on - strand, within H281DRAFT_04220at 339.322 kb on - strand, within H281DRAFT_04220at 339.346 kb on - strand, within H281DRAFT_04220at 339.381 kb on + strand, within H281DRAFT_04220at 339.381 kb on + strand, within H281DRAFT_04220at 339.382 kb on - strand, within H281DRAFT_04220at 339.382 kb on - strand, within H281DRAFT_04220at 339.382 kb on - strand, within H281DRAFT_04220at 339.382 kb on - strand, within H281DRAFT_04220at 339.504 kb on + strand, within H281DRAFT_04220at 339.505 kb on - strand, within H281DRAFT_04220at 339.663 kb on + strand, within H281DRAFT_04220at 339.807 kb on + strand, within H281DRAFT_04220at 339.807 kb on + strand, within H281DRAFT_04220at 339.808 kb on - strand, within H281DRAFT_04220at 339.808 kb on - strand, within H281DRAFT_04220at 339.945 kb on + strand, within H281DRAFT_04220at 340.156 kb on + strandat 340.156 kb on + strandat 340.157 kb on - strandat 340.173 kb on + strandat 340.173 kb on + strandat 340.173 kb on + strandat 340.173 kb on + strandat 340.173 kb on + strandat 340.181 kb on + strandat 340.254 kb on + strandat 340.255 kb on - strandat 340.413 kb on - strandat 340.491 kb on + strand, within H281DRAFT_04221at 340.491 kb on + strand, within H281DRAFT_04221at 340.491 kb on + strand, within H281DRAFT_04221at 340.491 kb on + strand, within H281DRAFT_04221at 340.491 kb on + strand, within H281DRAFT_04221at 340.492 kb on - strand, within H281DRAFT_04221at 340.492 kb on - strand, within H281DRAFT_04221at 340.492 kb on - strand, within H281DRAFT_04221at 340.515 kb on + strand, within H281DRAFT_04221at 340.515 kb on + strand, within H281DRAFT_04221at 340.515 kb on + strand, within H281DRAFT_04221at 340.539 kb on + strand, within H281DRAFT_04221at 340.539 kb on + strand, within H281DRAFT_04221at 340.539 kb on + strand, within H281DRAFT_04221at 340.540 kb on - strand, within H281DRAFT_04221at 340.540 kb on - strand, within H281DRAFT_04221at 340.544 kb on + strand, within H281DRAFT_04221at 340.544 kb on + strand, within H281DRAFT_04221at 340.558 kb on - strand, within H281DRAFT_04221at 340.558 kb on - strand, within H281DRAFT_04221at 340.558 kb on - strand, within H281DRAFT_04221at 340.581 kb on - strand, within H281DRAFT_04221at 340.621 kb on - strand, within H281DRAFT_04221at 340.641 kb on + strand, within H281DRAFT_04221at 340.641 kb on + strand, within H281DRAFT_04221at 340.641 kb on + strand, within H281DRAFT_04221at 340.642 kb on - strand, within H281DRAFT_04221at 340.779 kb on + strand, within H281DRAFT_04221at 340.779 kb on + strand, within H281DRAFT_04221at 340.780 kb on - strand, within H281DRAFT_04221at 340.780 kb on - strand, within H281DRAFT_04221at 340.780 kb on - strand, within H281DRAFT_04221at 340.780 kb on - strand, within H281DRAFT_04221at 340.780 kb on - strand, within H281DRAFT_04221at 340.878 kb on + strand, within H281DRAFT_04221at 340.878 kb on + strand, within H281DRAFT_04221at 340.879 kb on - strand, within H281DRAFT_04221at 340.879 kb on - strand, within H281DRAFT_04221at 340.879 kb on - strand, within H281DRAFT_04221at 340.879 kb on - strand, within H281DRAFT_04221at 340.893 kb on + strand, within H281DRAFT_04221at 340.894 kb on - strand, within H281DRAFT_04221at 340.933 kb on + strand, within H281DRAFT_04221at 340.934 kb on + strand, within H281DRAFT_04221at 340.935 kb on - strand, within H281DRAFT_04221at 340.935 kb on - strand, within H281DRAFT_04221at 340.935 kb on - strand, within H281DRAFT_04221at 340.951 kb on + strandat 341.029 kb on - strandat 341.220 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)
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338,106 + -0.1
338,107 - -0.3
338,107 - +0.1
338,130 - +0.6
338,212 + +1.9
338,213 - +0.4
338,280 + +1.7
338,280 + +0.5
338,281 - +0.7
338,281 - +2.1
338,281 - +0.4
338,281 - -0.4
338,282 + +2.4
338,322 - H281DRAFT_04219 0.15 +0.5
338,325 - +0.9
338,378 + -0.7
338,378 + H281DRAFT_04219 0.22 +0.7
338,424 + H281DRAFT_04219 0.29 +1.0
338,425 - H281DRAFT_04219 0.29 +0.5
338,430 + H281DRAFT_04219 0.30 +0.8
338,431 - H281DRAFT_04219 0.30 +2.3
338,431 - H281DRAFT_04219 0.30 +0.9
338,484 + H281DRAFT_04219 0.37 +0.6
338,485 - H281DRAFT_04219 0.37 +0.4
338,485 - H281DRAFT_04219 0.37 -0.2
338,497 - H281DRAFT_04219 0.39 +0.2
338,511 + H281DRAFT_04219 0.41 +1.0
338,512 - H281DRAFT_04219 0.41 -0.1
338,532 + H281DRAFT_04219 0.44 +0.7
338,532 + H281DRAFT_04219 0.44 +0.4
338,532 + H281DRAFT_04219 0.44 +0.2
338,533 - H281DRAFT_04219 0.44 +3.2
338,533 - H281DRAFT_04219 0.44 +1.5
338,728 - H281DRAFT_04219 0.71 +1.4
339,114 + +1.9
339,114 + H281DRAFT_04220 0.12 +0.9
339,115 - H281DRAFT_04220 0.12 +1.0
339,138 - H281DRAFT_04220 0.14 +0.2
339,322 - H281DRAFT_04220 0.28 +1.2
339,346 - H281DRAFT_04220 0.30 +0.3
339,381 + H281DRAFT_04220 0.33 +1.2
339,381 + H281DRAFT_04220 0.33 +1.4
339,382 - H281DRAFT_04220 0.33 +1.1
339,382 - H281DRAFT_04220 0.33 +2.2
339,382 - H281DRAFT_04220 0.33 -0.1
339,382 - H281DRAFT_04220 0.33 +1.1
339,504 + H281DRAFT_04220 0.42 +2.3
339,505 - H281DRAFT_04220 0.42 +2.0
339,663 + H281DRAFT_04220 0.54 +2.3
339,807 + H281DRAFT_04220 0.65 +1.0
339,807 + H281DRAFT_04220 0.65 +1.1
339,808 - H281DRAFT_04220 0.65 +1.4
339,808 - H281DRAFT_04220 0.65 +1.7
339,945 + H281DRAFT_04220 0.76 +0.7
340,156 + +0.3
340,156 + +0.3
340,157 - +1.6
340,173 + +0.5
340,173 + +0.8
340,173 + +2.2
340,173 + +0.8
340,173 + +0.0
340,181 + -0.1
340,254 + -0.2
340,255 - -0.7
340,413 - +0.1
340,491 + H281DRAFT_04221 0.17 +0.2
340,491 + H281DRAFT_04221 0.17 +0.2
340,491 + H281DRAFT_04221 0.17 -0.1
340,491 + H281DRAFT_04221 0.17 -0.3
340,491 + H281DRAFT_04221 0.17 -0.2
340,492 - H281DRAFT_04221 0.18 +0.3
340,492 - H281DRAFT_04221 0.18 +1.1
340,492 - H281DRAFT_04221 0.18 +0.5
340,515 + H281DRAFT_04221 0.21 +1.7
340,515 + H281DRAFT_04221 0.21 -0.9
340,515 + H281DRAFT_04221 0.21 -0.2
340,539 + H281DRAFT_04221 0.25 -0.8
340,539 + H281DRAFT_04221 0.25 -0.2
340,539 + H281DRAFT_04221 0.25 -0.7
340,540 - H281DRAFT_04221 0.25 +0.4
340,540 - H281DRAFT_04221 0.25 +0.0
340,544 + H281DRAFT_04221 0.26 -0.7
340,544 + H281DRAFT_04221 0.26 +0.9
340,558 - H281DRAFT_04221 0.28 -0.7
340,558 - H281DRAFT_04221 0.28 +0.4
340,558 - H281DRAFT_04221 0.28 -1.1
340,581 - H281DRAFT_04221 0.32 -0.1
340,621 - H281DRAFT_04221 0.38 +0.2
340,641 + H281DRAFT_04221 0.42 -0.8
340,641 + H281DRAFT_04221 0.42 -0.4
340,641 + H281DRAFT_04221 0.42 -0.2
340,642 - H281DRAFT_04221 0.42 -0.2
340,779 + H281DRAFT_04221 0.64 +0.2
340,779 + H281DRAFT_04221 0.64 +0.5
340,780 - H281DRAFT_04221 0.64 +0.9
340,780 - H281DRAFT_04221 0.64 -1.1
340,780 - H281DRAFT_04221 0.64 -0.1
340,780 - H281DRAFT_04221 0.64 -0.4
340,780 - H281DRAFT_04221 0.64 +0.1
340,878 + H281DRAFT_04221 0.80 -0.8
340,878 + H281DRAFT_04221 0.80 +0.1
340,879 - H281DRAFT_04221 0.80 +0.7
340,879 - H281DRAFT_04221 0.80 +0.3
340,879 - H281DRAFT_04221 0.80 +0.0
340,879 - H281DRAFT_04221 0.80 +0.3
340,893 + H281DRAFT_04221 0.82 -1.1
340,894 - H281DRAFT_04221 0.82 +0.7
340,933 + H281DRAFT_04221 0.89 -1.2
340,934 + H281DRAFT_04221 0.89 -1.1
340,935 - H281DRAFT_04221 0.89 -0.1
340,935 - H281DRAFT_04221 0.89 +0.5
340,935 - H281DRAFT_04221 0.89 +1.2
340,951 + +1.2
341,029 - +0.0
341,220 + -0.7

Or see this region's nucleotide sequence