Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_03421

Experiment: 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_03420 and H281DRAFT_03421 are separated by 51 nucleotidesH281DRAFT_03421 and H281DRAFT_03422 are separated by 222 nucleotides H281DRAFT_03420: H281DRAFT_03420 - 4-oxalomesaconate hydratase (EC 4.2.1.83), at 493,572 to 494,597 _03420 H281DRAFT_03421: H281DRAFT_03421 - transcriptional regulator, IclR family, at 494,649 to 495,494 _03421 H281DRAFT_03422: H281DRAFT_03422 - dihydroxyacid dehydratase (EC 4.2.1.9), at 495,717 to 497,495 _03422 Position (kb) 494 495 496Strain fitness (log2 ratio) -2 -1 0 1at 493.779 kb on + strand, within H281DRAFT_03420at 493.780 kb on - strand, within H281DRAFT_03420at 493.780 kb on - strand, within H281DRAFT_03420at 493.787 kb on + strand, within H281DRAFT_03420at 494.018 kb on - strand, within H281DRAFT_03420at 494.087 kb on - strand, within H281DRAFT_03420at 495.230 kb on - strand, within H281DRAFT_03421at 495.240 kb on + strand, within H281DRAFT_03421at 495.240 kb on + strand, within H281DRAFT_03421at 495.240 kb on + strand, within H281DRAFT_03421at 495.344 kb on - strand, within H281DRAFT_03421at 495.483 kb on - strandat 495.494 kb on + strandat 495.494 kb on + strandat 495.495 kb on - strandat 495.584 kb on + strandat 495.606 kb on + strandat 495.762 kb on + strandat 495.834 kb on + strandat 496.015 kb on - strand, within H281DRAFT_03422at 496.468 kb on + strand, within H281DRAFT_03422at 496.469 kb on - strand, within H281DRAFT_03422

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)
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493,779 + H281DRAFT_03420 0.20 -0.7
493,780 - H281DRAFT_03420 0.20 -0.0
493,780 - H281DRAFT_03420 0.20 +0.6
493,787 + H281DRAFT_03420 0.21 -0.2
494,018 - H281DRAFT_03420 0.43 +0.4
494,087 - H281DRAFT_03420 0.50 +1.1
495,230 - H281DRAFT_03421 0.69 +1.0
495,240 + H281DRAFT_03421 0.70 -0.0
495,240 + H281DRAFT_03421 0.70 +0.9
495,240 + H281DRAFT_03421 0.70 +0.5
495,344 - H281DRAFT_03421 0.82 -1.1
495,483 - -0.9
495,494 + +0.2
495,494 + +0.8
495,495 - +0.6
495,584 + +1.5
495,606 + +0.1
495,762 + -2.6
495,834 + +0.7
496,015 - H281DRAFT_03422 0.17 -0.0
496,468 + H281DRAFT_03422 0.42 +0.4
496,469 - H281DRAFT_03422 0.42 +1.1

Or see this region's nucleotide sequence