Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_00201

Experiment: 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_00200 and H281DRAFT_00201 are separated by 199 nucleotidesH281DRAFT_00201 and H281DRAFT_00202 are separated by 158 nucleotidesH281DRAFT_00202 and H281DRAFT_00203 are separated by 12 nucleotides H281DRAFT_00200: H281DRAFT_00200 - HemY protein, at 218,070 to 219,260 _00200 H281DRAFT_00201: H281DRAFT_00201 - hypothetical protein, at 219,460 to 219,777 _00201 H281DRAFT_00202: H281DRAFT_00202 - RNA polymerase sigma factor, sigma-70 family, at 219,936 to 220,517 _00202 H281DRAFT_00203: H281DRAFT_00203 - hypothetical protein, at 220,530 to 220,838 _00203 Position (kb) 219 220Strain fitness (log2 ratio) -2 -1 0 1at 218.475 kb on + strandat 218.867 kb on - strand, within H281DRAFT_00200at 219.436 kb on + strandat 219.461 kb on + strandat 219.461 kb on + strandat 219.495 kb on + strand, within H281DRAFT_00201at 219.496 kb on - strand, within H281DRAFT_00201at 219.768 kb on - strandat 219.811 kb on + strandat 220.069 kb on + strand, within H281DRAFT_00202at 220.069 kb on + strand, within H281DRAFT_00202at 220.070 kb on - strand, within H281DRAFT_00202at 220.070 kb on - strand, within H281DRAFT_00202at 220.070 kb on - strand, within H281DRAFT_00202at 220.296 kb on + strand, within H281DRAFT_00202at 220.436 kb on + strandat 220.437 kb on - strand, within H281DRAFT_00202at 220.515 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)
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218,475 + +0.2
218,867 - H281DRAFT_00200 0.67 -0.2
219,436 + -1.8
219,461 + -0.4
219,461 + +1.4
219,495 + H281DRAFT_00201 0.11 +0.7
219,496 - H281DRAFT_00201 0.11 +0.8
219,768 - +0.7
219,811 + +0.1
220,069 + H281DRAFT_00202 0.23 -0.9
220,069 + H281DRAFT_00202 0.23 +0.6
220,070 - H281DRAFT_00202 0.23 +0.1
220,070 - H281DRAFT_00202 0.23 +1.5
220,070 - H281DRAFT_00202 0.23 -0.4
220,296 + H281DRAFT_00202 0.62 -0.3
220,436 + +0.8
220,437 - H281DRAFT_00202 0.86 -0.2
220,515 + +0.3

Or see this region's nucleotide sequence