Experiment: ICP1_phage_235.3_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_05210 and CSW01_05215 are separated by 59 nucleotides CSW01_05215 and CSW01_05220 are separated by 10 nucleotides CSW01_05220 and CSW01_05225 overlap by 4 nucleotides CSW01_05225 and CSW01_05230 are separated by 1 nucleotides
CSW01_05210: CSW01_05210 - GTP 3',8-cyclase MoaA, at 1,091,888 to 1,092,877
_05210
CSW01_05215: CSW01_05215 - molybdenum cofactor biosynthesis protein B, at 1,092,937 to 1,093,449
_05215
CSW01_05220: CSW01_05220 - cyclic pyranopterin monophosphate synthase MoaC, at 1,093,460 to 1,093,939
_05220
CSW01_05225: CSW01_05225 - molybdopterin synthase sulfur carrier subunit, at 1,093,936 to 1,094,181
_05225
CSW01_05230: CSW01_05230 - molybdopterin synthase catalytic subunit, at 1,094,183 to 1,094,635
_05230
Position (kb)
1092
1093
1094 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1091.998 kb on + strand, within CSW01_05210 at 1092.264 kb on + strand, within CSW01_05210 at 1092.264 kb on + strand, within CSW01_05210 at 1092.264 kb on + strand, within CSW01_05210 at 1092.264 kb on + strand, within CSW01_05210 at 1092.272 kb on - strand, within CSW01_05210 at 1092.272 kb on - strand, within CSW01_05210 at 1092.272 kb on - strand, within CSW01_05210 at 1092.272 kb on - strand, within CSW01_05210 at 1092.339 kb on + strand, within CSW01_05210 at 1092.372 kb on + strand, within CSW01_05210 at 1092.399 kb on + strand, within CSW01_05210 at 1092.399 kb on + strand, within CSW01_05210 at 1092.407 kb on - strand, within CSW01_05210 at 1092.407 kb on - strand, within CSW01_05210 at 1092.407 kb on - strand, within CSW01_05210 at 1092.407 kb on - strand, within CSW01_05210 at 1092.557 kb on + strand, within CSW01_05210 at 1092.582 kb on + strand, within CSW01_05210 at 1092.593 kb on + strand, within CSW01_05210 at 1092.862 kb on - strand at 1092.909 kb on + strand at 1092.909 kb on + strand at 1092.909 kb on + strand at 1093.034 kb on + strand, within CSW01_05215 at 1093.052 kb on - strand, within CSW01_05215 at 1093.169 kb on + strand, within CSW01_05215 at 1093.213 kb on - strand, within CSW01_05215 at 1093.214 kb on - strand, within CSW01_05215 at 1093.265 kb on + strand, within CSW01_05215 at 1093.273 kb on - strand, within CSW01_05215 at 1093.275 kb on + strand, within CSW01_05215 at 1093.283 kb on - strand, within CSW01_05215 at 1093.306 kb on + strand, within CSW01_05215 at 1093.306 kb on + strand, within CSW01_05215 at 1093.449 kb on + strand at 1093.462 kb on - strand at 1093.472 kb on - strand at 1093.477 kb on + strand at 1093.508 kb on - strand, within CSW01_05220 at 1093.522 kb on + strand, within CSW01_05220 at 1093.606 kb on - strand, within CSW01_05220 at 1093.614 kb on - strand, within CSW01_05220 at 1093.708 kb on + strand, within CSW01_05220 at 1093.716 kb on - strand, within CSW01_05220 at 1093.716 kb on - strand, within CSW01_05220 at 1093.720 kb on + strand, within CSW01_05220 at 1093.720 kb on + strand, within CSW01_05220 at 1093.729 kb on - strand, within CSW01_05220 at 1093.834 kb on - strand, within CSW01_05220 at 1093.846 kb on + strand, within CSW01_05220 at 1093.854 kb on - strand, within CSW01_05220 at 1093.869 kb on + strand, within CSW01_05220 at 1094.016 kb on + strand, within CSW01_05225 at 1094.024 kb on - strand, within CSW01_05225 at 1094.024 kb on - strand, within CSW01_05225 at 1094.053 kb on + strand, within CSW01_05225 at 1094.118 kb on + strand, within CSW01_05225 at 1094.171 kb on + strand at 1094.253 kb on + strand, within CSW01_05230 at 1094.332 kb on - strand, within CSW01_05230
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_235.3_MOI_rep1 remove 1,091,998 + CSW01_05210 0.11 +0.1 1,092,264 + CSW01_05210 0.38 +0.1 1,092,264 + CSW01_05210 0.38 -1.2 1,092,264 + CSW01_05210 0.38 +0.2 1,092,264 + CSW01_05210 0.38 +1.0 1,092,272 - CSW01_05210 0.39 -0.6 1,092,272 - CSW01_05210 0.39 +2.0 1,092,272 - CSW01_05210 0.39 -2.8 1,092,272 - CSW01_05210 0.39 -1.4 1,092,339 + CSW01_05210 0.46 -0.1 1,092,372 + CSW01_05210 0.49 -0.9 1,092,399 + CSW01_05210 0.52 -1.3 1,092,399 + CSW01_05210 0.52 +1.5 1,092,407 - CSW01_05210 0.52 +0.5 1,092,407 - CSW01_05210 0.52 -0.5 1,092,407 - CSW01_05210 0.52 -2.6 1,092,407 - CSW01_05210 0.52 -2.5 1,092,557 + CSW01_05210 0.68 -0.3 1,092,582 + CSW01_05210 0.70 +1.3 1,092,593 + CSW01_05210 0.71 +0.3 1,092,862 - +0.8 1,092,909 + +1.0 1,092,909 + +0.4 1,092,909 + -0.6 1,093,034 + CSW01_05215 0.19 -1.3 1,093,052 - CSW01_05215 0.22 +0.2 1,093,169 + CSW01_05215 0.45 -0.9 1,093,213 - CSW01_05215 0.54 -1.3 1,093,214 - CSW01_05215 0.54 +1.4 1,093,265 + CSW01_05215 0.64 -0.6 1,093,273 - CSW01_05215 0.65 -0.5 1,093,275 + CSW01_05215 0.66 +1.0 1,093,283 - CSW01_05215 0.67 +0.5 1,093,306 + CSW01_05215 0.72 -2.0 1,093,306 + CSW01_05215 0.72 -1.4 1,093,449 + +0.2 1,093,462 - -1.7 1,093,472 - +0.0 1,093,477 + -0.2 1,093,508 - CSW01_05220 0.10 -0.3 1,093,522 + CSW01_05220 0.13 -2.1 1,093,606 - CSW01_05220 0.30 -0.4 1,093,614 - CSW01_05220 0.32 -1.7 1,093,708 + CSW01_05220 0.52 +1.1 1,093,716 - CSW01_05220 0.53 -1.4 1,093,716 - CSW01_05220 0.53 -1.6 1,093,720 + CSW01_05220 0.54 -0.1 1,093,720 + CSW01_05220 0.54 -1.1 1,093,729 - CSW01_05220 0.56 -1.7 1,093,834 - CSW01_05220 0.78 -0.4 1,093,846 + CSW01_05220 0.80 +0.1 1,093,854 - CSW01_05220 0.82 -0.0 1,093,869 + CSW01_05220 0.85 +0.2 1,094,016 + CSW01_05225 0.33 -0.4 1,094,024 - CSW01_05225 0.36 +1.4 1,094,024 - CSW01_05225 0.36 -2.3 1,094,053 + CSW01_05225 0.48 -1.9 1,094,118 + CSW01_05225 0.74 -0.4 1,094,171 + -0.4 1,094,253 + CSW01_05230 0.15 +0.5 1,094,332 - CSW01_05230 0.33 +0.9
Or see this region's nucleotide sequence