Experiment: ICP1_phage_235.3_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_02800 and CSW01_02805 overlap by 4 nucleotides CSW01_02805 and CSW01_02810 overlap by 4 nucleotides CSW01_02810 and CSW01_02815 are separated by 29 nucleotides
CSW01_02800: CSW01_02800 - cell division protein FtsB, at 558,518 to 558,808
_02800
CSW01_02805: CSW01_02805 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 558,805 to 559,503
_02805
CSW01_02810: CSW01_02810 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 559,500 to 559,976
_02810
CSW01_02815: CSW01_02815 - tRNA pseudouridine(13) synthase TruD, at 560,006 to 561,091
_02815
Position (kb)
559
560 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 558.506 kb on - strand at 558.515 kb on + strand at 558.789 kb on + strand at 558.802 kb on + strand at 558.807 kb on + strand at 559.971 kb on + strand at 559.971 kb on + strand at 559.979 kb on - strand at 559.979 kb on - strand at 560.032 kb on - strand at 560.059 kb on + strand at 560.059 kb on + strand at 560.086 kb on + strand at 560.102 kb on + strand at 560.102 kb on + strand at 560.110 kb on - strand at 560.136 kb on + strand, within CSW01_02815 at 560.162 kb on + strand, within CSW01_02815 at 560.162 kb on + strand, within CSW01_02815 at 560.172 kb on - strand, within CSW01_02815 at 560.269 kb on + strand, within CSW01_02815 at 560.269 kb on + strand, within CSW01_02815 at 560.277 kb on - strand, within CSW01_02815 at 560.327 kb on + strand, within CSW01_02815 at 560.444 kb on + strand, within CSW01_02815 at 560.444 kb on + strand, within CSW01_02815 at 560.444 kb on + strand, within CSW01_02815 at 560.444 kb on + strand, within CSW01_02815 at 560.444 kb on + strand, within CSW01_02815 at 560.444 kb on + strand, within CSW01_02815 at 560.444 kb on + strand, within CSW01_02815 at 560.444 kb on + strand, within CSW01_02815 at 560.444 kb on + strand, within CSW01_02815 at 560.452 kb on - strand, within CSW01_02815 at 560.452 kb on - strand, within CSW01_02815 at 560.452 kb on - strand, within CSW01_02815 at 560.452 kb on - strand, within CSW01_02815 at 560.452 kb on - strand, within CSW01_02815 at 560.452 kb on - strand, within CSW01_02815 at 560.452 kb on - strand, within CSW01_02815 at 560.452 kb on - strand, within CSW01_02815 at 560.452 kb on - strand, within CSW01_02815 at 560.452 kb on - strand, within CSW01_02815 at 560.452 kb on - strand, within CSW01_02815 at 560.454 kb on - strand, within CSW01_02815 at 560.520 kb on + strand, within CSW01_02815 at 560.527 kb on + strand, within CSW01_02815 at 560.552 kb on - strand, within CSW01_02815 at 560.589 kb on + strand, within CSW01_02815 at 560.589 kb on + strand, within CSW01_02815 at 560.589 kb on + strand, within CSW01_02815 at 560.589 kb on + strand, within CSW01_02815 at 560.589 kb on + strand, within CSW01_02815 at 560.589 kb on + strand, within CSW01_02815 at 560.589 kb on + strand, within CSW01_02815 at 560.589 kb on + strand, within CSW01_02815 at 560.589 kb on + strand, within CSW01_02815 at 560.597 kb on - strand, within CSW01_02815 at 560.597 kb on - strand, within CSW01_02815 at 560.597 kb on - strand, within CSW01_02815 at 560.597 kb on - strand, within CSW01_02815 at 560.597 kb on - strand, within CSW01_02815 at 560.597 kb on - strand, within CSW01_02815 at 560.597 kb on - strand, within CSW01_02815 at 560.607 kb on - strand, within CSW01_02815 at 560.653 kb on + strand, within CSW01_02815 at 560.661 kb on - strand, within CSW01_02815 at 560.724 kb on + strand, within CSW01_02815 at 560.737 kb on + strand, within CSW01_02815 at 560.796 kb on - strand, within CSW01_02815 at 560.810 kb on + strand, within CSW01_02815 at 560.810 kb on + strand, within CSW01_02815 at 560.818 kb on - strand, within CSW01_02815 at 560.956 kb on - strand, within CSW01_02815 at 560.975 kb on + strand, within CSW01_02815 at 560.975 kb on + strand, within CSW01_02815
Per-strain Table
Position Strand Gene LocusTag Fraction ICP1_phage_235.3_MOI_rep1 remove 558,506 - -1.2 558,515 + -2.7 558,789 + +1.7 558,802 + -1.8 558,807 + +1.0 559,971 + +1.4 559,971 + -1.3 559,979 - -2.0 559,979 - -2.4 560,032 - -1.0 560,059 + +1.8 560,059 + -0.3 560,086 + +0.2 560,102 + +0.3 560,102 + +0.6 560,110 - +1.0 560,136 + CSW01_02815 0.12 -3.1 560,162 + CSW01_02815 0.14 +1.1 560,162 + CSW01_02815 0.14 -1.2 560,172 - CSW01_02815 0.15 -2.9 560,269 + CSW01_02815 0.24 -1.0 560,269 + CSW01_02815 0.24 +1.2 560,277 - CSW01_02815 0.25 -0.1 560,327 + CSW01_02815 0.30 +1.6 560,444 + CSW01_02815 0.40 +0.1 560,444 + CSW01_02815 0.40 +0.5 560,444 + CSW01_02815 0.40 -2.6 560,444 + CSW01_02815 0.40 +1.1 560,444 + CSW01_02815 0.40 +0.7 560,444 + CSW01_02815 0.40 -0.3 560,444 + CSW01_02815 0.40 +0.6 560,444 + CSW01_02815 0.40 +0.4 560,444 + CSW01_02815 0.40 -1.1 560,452 - CSW01_02815 0.41 +0.4 560,452 - CSW01_02815 0.41 -1.7 560,452 - CSW01_02815 0.41 +0.3 560,452 - CSW01_02815 0.41 +0.9 560,452 - CSW01_02815 0.41 -2.2 560,452 - CSW01_02815 0.41 +1.7 560,452 - CSW01_02815 0.41 -3.6 560,452 - CSW01_02815 0.41 -0.2 560,452 - CSW01_02815 0.41 -1.0 560,452 - CSW01_02815 0.41 -3.0 560,452 - CSW01_02815 0.41 +0.7 560,454 - CSW01_02815 0.41 -0.1 560,520 + CSW01_02815 0.47 -1.1 560,527 + CSW01_02815 0.48 +1.4 560,552 - CSW01_02815 0.50 +0.5 560,589 + CSW01_02815 0.54 -2.0 560,589 + CSW01_02815 0.54 +0.8 560,589 + CSW01_02815 0.54 -0.0 560,589 + CSW01_02815 0.54 +1.0 560,589 + CSW01_02815 0.54 +0.9 560,589 + CSW01_02815 0.54 +1.3 560,589 + CSW01_02815 0.54 -0.4 560,589 + CSW01_02815 0.54 +2.1 560,589 + CSW01_02815 0.54 +2.0 560,597 - CSW01_02815 0.54 +0.1 560,597 - CSW01_02815 0.54 +0.3 560,597 - CSW01_02815 0.54 +2.0 560,597 - CSW01_02815 0.54 +1.2 560,597 - CSW01_02815 0.54 -0.6 560,597 - CSW01_02815 0.54 +0.3 560,597 - CSW01_02815 0.54 -1.1 560,607 - CSW01_02815 0.55 -1.0 560,653 + CSW01_02815 0.60 -1.2 560,661 - CSW01_02815 0.60 -0.7 560,724 + CSW01_02815 0.66 +0.1 560,737 + CSW01_02815 0.67 +0.8 560,796 - CSW01_02815 0.73 -4.0 560,810 + CSW01_02815 0.74 +1.4 560,810 + CSW01_02815 0.74 +0.6 560,818 - CSW01_02815 0.75 -1.9 560,956 - CSW01_02815 0.87 -0.4 560,975 + CSW01_02815 0.89 -1.0 560,975 + CSW01_02815 0.89 +1.1
Or see this region's nucleotide sequence