Experiment: Tetracycline 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3201 and Shewana3_3202 are separated by 46 nucleotides Shewana3_3202 and djlA are separated by 55 nucleotides djlA and Shewana3_3204 are separated by 105 nucleotides Shewana3_3204 and Shewana3_3205 overlap by 4 nucleotides
Shewana3_3201: Shewana3_3201 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding (RefSeq), at 3,844,358 to 3,845,311
_3201
Shewana3_3202: Shewana3_3202 - hypothetical protein (RefSeq), at 3,845,358 to 3,846,002
_3202
Shewana3_3203: djlA - Dna-J like membrane chaperone protein (RefSeq), at 3,846,058 to 3,846,846
djlA
Shewana3_3204: Shewana3_3204 - nucleotidyl transferase (RefSeq), at 3,846,952 to 3,847,617
_3204
Shewana3_3205: Shewana3_3205 - aminoglycoside phosphotransferase (RefSeq), at 3,847,614 to 3,848,705
_3205
Position (kb)
3846
3847 Strain fitness (log2 ratio)
-2
-1
0
1 at 3845.088 kb on - strand, within Shewana3_3201 at 3845.706 kb on - strand, within Shewana3_3202 at 3845.910 kb on - strand, within Shewana3_3202 at 3845.952 kb on + strand at 3845.959 kb on - strand at 3846.088 kb on + strand at 3846.088 kb on + strand at 3846.088 kb on + strand at 3846.096 kb on - strand at 3846.137 kb on + strand, within djlA at 3846.328 kb on - strand, within djlA at 3846.355 kb on + strand, within djlA at 3846.356 kb on + strand, within djlA at 3846.369 kb on - strand, within djlA at 3846.477 kb on - strand, within djlA at 3846.510 kb on + strand, within djlA at 3846.687 kb on - strand, within djlA at 3846.833 kb on + strand at 3846.958 kb on - strand at 3847.020 kb on + strand, within Shewana3_3204 at 3847.082 kb on + strand, within Shewana3_3204 at 3847.117 kb on + strand, within Shewana3_3204 at 3847.242 kb on - strand, within Shewana3_3204 at 3847.292 kb on + strand, within Shewana3_3204 at 3847.345 kb on - strand, within Shewana3_3204 at 3847.422 kb on + strand, within Shewana3_3204 at 3847.589 kb on - strand at 3847.708 kb on + strand at 3847.716 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.001 mg/ml remove 3,845,088 - Shewana3_3201 0.77 -1.8 3,845,706 - Shewana3_3202 0.54 +0.4 3,845,910 - Shewana3_3202 0.86 +0.1 3,845,952 + +0.5 3,845,959 - -0.1 3,846,088 + +0.0 3,846,088 + +1.0 3,846,088 + -0.6 3,846,096 - -0.6 3,846,137 + djlA Shewana3_3203 0.10 +1.0 3,846,328 - djlA Shewana3_3203 0.34 -0.6 3,846,355 + djlA Shewana3_3203 0.38 +1.0 3,846,356 + djlA Shewana3_3203 0.38 +1.0 3,846,369 - djlA Shewana3_3203 0.39 -0.5 3,846,477 - djlA Shewana3_3203 0.53 -0.3 3,846,510 + djlA Shewana3_3203 0.57 +1.0 3,846,687 - djlA Shewana3_3203 0.80 -0.6 3,846,833 + +1.0 3,846,958 - -0.4 3,847,020 + Shewana3_3204 0.10 -0.6 3,847,082 + Shewana3_3204 0.20 +0.0 3,847,117 + Shewana3_3204 0.25 +1.0 3,847,242 - Shewana3_3204 0.44 -0.5 3,847,292 + Shewana3_3204 0.51 +0.2 3,847,345 - Shewana3_3204 0.59 -0.4 3,847,422 + Shewana3_3204 0.71 +1.0 3,847,589 - -1.2 3,847,708 + +0.2 3,847,716 - +0.5
Or see this region's nucleotide sequence