Experiment: Tetracycline 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1753 and infA are separated by 103 nucleotides infA and Shewana3_1755 are separated by 82 nucleotides Shewana3_1755 and aat overlap by 11 nucleotides aat and Shewana3_1757 are separated by 81 nucleotides
Shewana3_1753: Shewana3_1753 - ATP-dependent Clp protease, ATP-binding subunit clpA (RefSeq), at 2,067,010 to 2,069,277
_1753
Shewana3_1754: infA - translation initiation factor IF-1 (RefSeq), at 2,069,381 to 2,069,599
infA
Shewana3_1755: Shewana3_1755 - arginyl-tRNA-protein transferase (RefSeq), at 2,069,682 to 2,070,398
_1755
Shewana3_1756: aat - leucyl/phenylalanyl-tRNA--protein transferase (RefSeq), at 2,070,388 to 2,071,098
aat
Shewana3_1757: Shewana3_1757 - 17 kDa surface antigen (RefSeq), at 2,071,180 to 2,071,623
_1757
Position (kb)
2069
2070
2071 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2068.865 kb on + strand, within Shewana3_1753 at 2069.714 kb on - strand at 2069.744 kb on - strand at 2069.846 kb on + strand, within Shewana3_1755 at 2069.918 kb on - strand, within Shewana3_1755 at 2070.092 kb on + strand, within Shewana3_1755 at 2070.092 kb on + strand, within Shewana3_1755 at 2070.294 kb on - strand, within Shewana3_1755 at 2070.451 kb on - strand at 2070.451 kb on - strand at 2070.463 kb on - strand, within aat at 2070.510 kb on - strand, within aat at 2071.334 kb on + strand, within Shewana3_1757 at 2071.398 kb on - strand, within Shewana3_1757
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.001 mg/ml remove 2,068,865 + Shewana3_1753 0.82 -1.0 2,069,714 - +1.1 2,069,744 - +0.8 2,069,846 + Shewana3_1755 0.23 -0.2 2,069,918 - Shewana3_1755 0.33 +2.8 2,070,092 + Shewana3_1755 0.57 +1.5 2,070,092 + Shewana3_1755 0.57 +0.8 2,070,294 - Shewana3_1755 0.85 +0.8 2,070,451 - +0.8 2,070,451 - +1.6 2,070,463 - aat Shewana3_1756 0.11 -0.2 2,070,510 - aat Shewana3_1756 0.17 +0.0 2,071,334 + Shewana3_1757 0.35 +0.4 2,071,398 - Shewana3_1757 0.49 +0.8
Or see this region's nucleotide sequence