Experiment: Tetracycline 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0617 and Shewana3_0618 are separated by 422 nucleotides Shewana3_0618 and Shewana3_0619 are separated by 120 nucleotides Shewana3_0619 and Shewana3_0620 are separated by 170 nucleotides
Shewana3_0617: Shewana3_0617 - cAMP-regulatory protein (RefSeq), at 722,231 to 722,866
_0617
Shewana3_0618: Shewana3_0618 - Sel1 domain-containing protein (RefSeq), at 723,289 to 724,068
_0618
Shewana3_0619: Shewana3_0619 - 2'-5' RNA ligase (RefSeq), at 724,189 to 724,761
_0619
Shewana3_0620: Shewana3_0620 - diguanylate cyclase/phosphodiesterase (RefSeq), at 724,932 to 727,118
_0620
Position (kb) 723
724
725 Strain fitness (log2 ratio) -1
0
1
2
3 at 722.711 kb on - strand, within Shewana3_0617 at 723.001 kb on - strand at 723.059 kb on + strand at 723.065 kb on + strand at 723.065 kb on + strand at 723.073 kb on + strand at 723.073 kb on + strand at 723.073 kb on + strand at 723.073 kb on - strand at 723.073 kb on - strand at 723.327 kb on - strand at 723.339 kb on + strand at 723.344 kb on + strand at 723.578 kb on + strand, within Shewana3_0618 at 723.641 kb on - strand, within Shewana3_0618 at 723.709 kb on + strand, within Shewana3_0618 at 724.180 kb on + strand at 724.266 kb on + strand, within Shewana3_0619 at 724.266 kb on + strand, within Shewana3_0619 at 724.291 kb on + strand, within Shewana3_0619 at 724.326 kb on + strand, within Shewana3_0619 at 724.334 kb on - strand, within Shewana3_0619 at 724.334 kb on - strand, within Shewana3_0619 at 724.441 kb on - strand, within Shewana3_0619 at 724.517 kb on - strand, within Shewana3_0619 at 724.724 kb on - strand at 724.738 kb on + strand at 725.041 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.001 mg/ml remove 722,711 - Shewana3_0617 0.75 -1.4 723,001 - +0.1 723,059 + +0.6 723,065 + -0.1 723,065 + +0.1 723,073 + -0.1 723,073 + -0.4 723,073 + +0.5 723,073 - +0.4 723,073 - -1.1 723,327 - -0.2 723,339 + -0.4 723,344 + -0.5 723,578 + Shewana3_0618 0.37 +0.1 723,641 - Shewana3_0618 0.45 +1.2 723,709 + Shewana3_0618 0.54 -0.8 724,180 + -0.3 724,266 + Shewana3_0619 0.13 +0.4 724,266 + Shewana3_0619 0.13 +0.1 724,291 + Shewana3_0619 0.18 +1.7 724,326 + Shewana3_0619 0.24 -0.3 724,334 - Shewana3_0619 0.25 -0.3 724,334 - Shewana3_0619 0.25 +0.1 724,441 - Shewana3_0619 0.44 +0.0 724,517 - Shewana3_0619 0.57 +3.4 724,724 - +0.1 724,738 + +0.1 725,041 - +0.8
Or see this region's nucleotide sequence