Experiment: Tetracycline 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0602 and Shewana3_0603 overlap by 1 nucleotides Shewana3_0603 and sspA are separated by 96 nucleotides sspA and Shewana3_0605 overlap by 1 nucleotides Shewana3_0605 and Shewana3_0606 are separated by 155 nucleotides
Shewana3_0602: Shewana3_0602 - cytochrome b/b6 domain-containing protein (RefSeq), at 705,513 to 706,727
_0602
Shewana3_0603: Shewana3_0603 - cytochrome c1 (RefSeq), at 706,727 to 707,425
_0603
Shewana3_0604: sspA - stringent starvation protein A (RefSeq), at 707,522 to 708,151
sspA
Shewana3_0605: Shewana3_0605 - ClpXP protease specificity-enhancing factor (RefSeq), at 708,151 to 708,588
_0605
Shewana3_0606: Shewana3_0606 - aminodeoxychorismate synthase, glutamine amidotransferase subunit (RefSeq), at 708,744 to 709,319
_0606
Position (kb) 707
708
709 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 706.594 kb on + strand, within Shewana3_0602 at 707.094 kb on + strand, within Shewana3_0603 at 707.202 kb on + strand, within Shewana3_0603 at 707.414 kb on + strand at 707.506 kb on + strand at 707.728 kb on + strand, within sspA at 708.451 kb on + strand, within Shewana3_0605 at 708.603 kb on + strand at 708.678 kb on - strand at 708.810 kb on + strand, within Shewana3_0606 at 708.900 kb on - strand, within Shewana3_0606 at 709.085 kb on + strand, within Shewana3_0606
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.001 mg/ml remove 706,594 + Shewana3_0602 0.89 -3.0 707,094 + Shewana3_0603 0.53 -2.5 707,202 + Shewana3_0603 0.68 -2.3 707,414 + -1.5 707,506 + -0.4 707,728 + sspA Shewana3_0604 0.33 +0.2 708,451 + Shewana3_0605 0.68 -1.0 708,603 + -1.1 708,678 - -0.4 708,810 + Shewana3_0606 0.11 -0.3 708,900 - Shewana3_0606 0.27 +0.3 709,085 + Shewana3_0606 0.59 +0.1
Or see this region's nucleotide sequence