Experiment: Tetracycline 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0374 and Shewana3_0375 are separated by 29 nucleotides Shewana3_0375 and Shewana3_R0020 are separated by 208 nucleotides Shewana3_R0020 and Shewana3_R0021 are separated by 53 nucleotides Shewana3_R0021 and Shewana3_R0022 are separated by 14 nucleotides Shewana3_R0022 and Shewana3_R0023 are separated by 53 nucleotides Shewana3_R0023 and Shewana3_0376 are separated by 189 nucleotides
Shewana3_0374: Shewana3_0374 - ATP-dependent DNA helicase Rep (RefSeq), at 411,568 to 413,583
_0374
Shewana3_0375: Shewana3_0375 - diguanylate cyclase/phosphodiesterase with GAF sensor (RefSeq), at 413,613 to 416,243
_0375
Shewana3_R0020: Shewana3_R0020 - tRNA-Arg (RefSeq), at 416,452 to 416,528
_R0020
Shewana3_R0021: Shewana3_R0021 - tRNA-His (RefSeq), at 416,582 to 416,657
_R0021
Shewana3_R0022: Shewana3_R0022 - tRNA-Pro (RefSeq), at 416,672 to 416,748
_R0022
Shewana3_R0023: Shewana3_R0023 - tRNA-Pro (RefSeq), at 416,802 to 416,878
_R0023
Shewana3_0376: Shewana3_0376 - diguanylate cyclase/phosphodiesterase (RefSeq), at 417,068 to 418,987
_0376
Position (kb) 413
414
415
416
417 Strain fitness (log2 ratio) -1
0
1
2 at 413.616 kb on + strand at 413.789 kb on + strand at 413.944 kb on - strand, within Shewana3_0375 at 414.011 kb on + strand, within Shewana3_0375 at 414.011 kb on - strand, within Shewana3_0375 at 414.024 kb on - strand, within Shewana3_0375 at 414.024 kb on - strand, within Shewana3_0375 at 414.086 kb on + strand, within Shewana3_0375 at 414.163 kb on + strand, within Shewana3_0375 at 414.165 kb on - strand, within Shewana3_0375 at 414.245 kb on - strand, within Shewana3_0375 at 414.261 kb on - strand, within Shewana3_0375 at 414.441 kb on + strand, within Shewana3_0375 at 414.465 kb on - strand, within Shewana3_0375 at 414.549 kb on + strand, within Shewana3_0375 at 414.549 kb on + strand, within Shewana3_0375 at 414.550 kb on - strand, within Shewana3_0375 at 414.550 kb on - strand, within Shewana3_0375 at 414.556 kb on - strand, within Shewana3_0375 at 414.558 kb on - strand, within Shewana3_0375 at 414.618 kb on - strand, within Shewana3_0375 at 414.657 kb on + strand, within Shewana3_0375 at 414.671 kb on - strand, within Shewana3_0375 at 414.775 kb on + strand, within Shewana3_0375 at 414.783 kb on - strand, within Shewana3_0375 at 414.794 kb on + strand, within Shewana3_0375 at 414.802 kb on - strand, within Shewana3_0375 at 414.802 kb on - strand, within Shewana3_0375 at 414.960 kb on + strand, within Shewana3_0375 at 415.009 kb on - strand, within Shewana3_0375 at 415.382 kb on - strand, within Shewana3_0375 at 415.382 kb on - strand, within Shewana3_0375 at 415.422 kb on - strand, within Shewana3_0375 at 415.527 kb on + strand, within Shewana3_0375 at 415.534 kb on + strand, within Shewana3_0375 at 415.534 kb on + strand, within Shewana3_0375 at 415.597 kb on - strand, within Shewana3_0375 at 415.927 kb on + strand, within Shewana3_0375 at 416.057 kb on + strand at 416.148 kb on - strand at 417.114 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.001 mg/ml remove 413,616 + +0.1 413,789 + +0.3 413,944 - Shewana3_0375 0.13 -0.2 414,011 + Shewana3_0375 0.15 +0.1 414,011 - Shewana3_0375 0.15 +0.0 414,024 - Shewana3_0375 0.16 +0.2 414,024 - Shewana3_0375 0.16 +0.1 414,086 + Shewana3_0375 0.18 -0.3 414,163 + Shewana3_0375 0.21 +0.3 414,165 - Shewana3_0375 0.21 -1.7 414,245 - Shewana3_0375 0.24 -0.7 414,261 - Shewana3_0375 0.25 +0.4 414,441 + Shewana3_0375 0.31 +0.5 414,465 - Shewana3_0375 0.32 +0.3 414,549 + Shewana3_0375 0.36 +0.0 414,549 + Shewana3_0375 0.36 +0.3 414,550 - Shewana3_0375 0.36 +0.7 414,550 - Shewana3_0375 0.36 +0.1 414,556 - Shewana3_0375 0.36 +0.3 414,558 - Shewana3_0375 0.36 +0.9 414,618 - Shewana3_0375 0.38 +1.2 414,657 + Shewana3_0375 0.40 +0.1 414,671 - Shewana3_0375 0.40 -0.2 414,775 + Shewana3_0375 0.44 +0.3 414,783 - Shewana3_0375 0.44 +1.0 414,794 + Shewana3_0375 0.45 +2.1 414,802 - Shewana3_0375 0.45 +1.8 414,802 - Shewana3_0375 0.45 -0.2 414,960 + Shewana3_0375 0.51 -0.7 415,009 - Shewana3_0375 0.53 +0.3 415,382 - Shewana3_0375 0.67 +0.3 415,382 - Shewana3_0375 0.67 -1.0 415,422 - Shewana3_0375 0.69 -1.6 415,527 + Shewana3_0375 0.73 -0.0 415,534 + Shewana3_0375 0.73 -0.4 415,534 + Shewana3_0375 0.73 -0.4 415,597 - Shewana3_0375 0.75 -0.6 415,927 + Shewana3_0375 0.88 +0.7 416,057 + -0.1 416,148 - +0.0 417,114 + +0.2
Or see this region's nucleotide sequence