Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2455 and Pf1N1B4_2456 are separated by 14 nucleotides Pf1N1B4_2456 and Pf1N1B4_2457 overlap by 4 nucleotides
Pf1N1B4_2455 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 2,784,943 to 2,786,193
_2455
Pf1N1B4_2456 - Sarcosine oxidase delta subunit (EC 1.5.3.1), at 2,786,208 to 2,786,537
_2456
Pf1N1B4_2457 - Sarcosine oxidase alpha subunit (EC 1.5.3.1), at 2,786,534 to 2,789,551
_2457
Position (kb)
2786
2787 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2785.267 kb on + strand, within Pf1N1B4_2455 at 2785.267 kb on + strand, within Pf1N1B4_2455 at 2785.268 kb on - strand, within Pf1N1B4_2455 at 2785.268 kb on - strand, within Pf1N1B4_2455 at 2785.268 kb on - strand, within Pf1N1B4_2455 at 2785.789 kb on + strand, within Pf1N1B4_2455 at 2785.789 kb on + strand, within Pf1N1B4_2455 at 2785.790 kb on - strand, within Pf1N1B4_2455 at 2786.192 kb on - strand at 2786.207 kb on + strand at 2786.207 kb on + strand at 2786.208 kb on - strand at 2786.208 kb on - strand at 2786.217 kb on - strand at 2786.334 kb on + strand, within Pf1N1B4_2456 at 2786.334 kb on + strand, within Pf1N1B4_2456 at 2786.334 kb on + strand, within Pf1N1B4_2456 at 2786.335 kb on - strand, within Pf1N1B4_2456 at 2786.335 kb on - strand, within Pf1N1B4_2456 at 2786.406 kb on + strand, within Pf1N1B4_2456 at 2786.407 kb on - strand, within Pf1N1B4_2456 at 2786.436 kb on + strand, within Pf1N1B4_2456 at 2786.454 kb on + strand, within Pf1N1B4_2456 at 2786.454 kb on + strand, within Pf1N1B4_2456 at 2786.454 kb on + strand, within Pf1N1B4_2456 at 2786.533 kb on + strand at 2786.533 kb on + strand at 2786.534 kb on - strand at 2786.534 kb on - strand at 2786.534 kb on - strand at 2786.615 kb on + strand at 2786.615 kb on + strand at 2786.616 kb on - strand at 2786.693 kb on + strand at 2786.693 kb on + strand at 2786.694 kb on - strand at 2786.694 kb on - strand at 2786.788 kb on - strand at 2786.810 kb on + strand at 2786.811 kb on - strand at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.918 kb on + strand, within Pf1N1B4_2457 at 2786.933 kb on + strand at 2786.957 kb on + strand, within Pf1N1B4_2457 at 2786.957 kb on + strand, within Pf1N1B4_2457 at 2786.957 kb on + strand, within Pf1N1B4_2457 at 2786.966 kb on + strand, within Pf1N1B4_2457 at 2786.966 kb on + strand, within Pf1N1B4_2457 at 2786.966 kb on + strand, within Pf1N1B4_2457 at 2786.966 kb on + strand, within Pf1N1B4_2457 at 2786.967 kb on - strand, within Pf1N1B4_2457 at 2786.967 kb on - strand, within Pf1N1B4_2457 at 2786.967 kb on - strand, within Pf1N1B4_2457 at 2787.023 kb on + strand, within Pf1N1B4_2457 at 2787.024 kb on - strand, within Pf1N1B4_2457 at 2787.029 kb on + strand, within Pf1N1B4_2457 at 2787.030 kb on - strand at 2787.030 kb on - strand, within Pf1N1B4_2457 at 2787.289 kb on + strand, within Pf1N1B4_2457 at 2787.289 kb on + strand, within Pf1N1B4_2457 at 2787.289 kb on + strand, within Pf1N1B4_2457 at 2787.290 kb on - strand, within Pf1N1B4_2457 at 2787.290 kb on - strand, within Pf1N1B4_2457 at 2787.290 kb on - strand, within Pf1N1B4_2457 at 2787.390 kb on + strand, within Pf1N1B4_2457 at 2787.390 kb on + strand, within Pf1N1B4_2457 at 2787.391 kb on - strand, within Pf1N1B4_2457 at 2787.428 kb on + strand, within Pf1N1B4_2457 at 2787.428 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.498 kb on + strand, within Pf1N1B4_2457
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,785,267 + Pf1N1B4_2455 0.26 -1.5 2,785,267 + Pf1N1B4_2455 0.26 +0.4 2,785,268 - Pf1N1B4_2455 0.26 +0.4 2,785,268 - Pf1N1B4_2455 0.26 -1.0 2,785,268 - Pf1N1B4_2455 0.26 -1.3 2,785,789 + Pf1N1B4_2455 0.68 +1.6 2,785,789 + Pf1N1B4_2455 0.68 -1.3 2,785,790 - Pf1N1B4_2455 0.68 -0.2 2,786,192 - +0.9 2,786,207 + +0.0 2,786,207 + +0.6 2,786,208 - +1.0 2,786,208 - -0.4 2,786,217 - +0.0 2,786,334 + Pf1N1B4_2456 0.38 +0.6 2,786,334 + Pf1N1B4_2456 0.38 +1.4 2,786,334 + Pf1N1B4_2456 0.38 +1.6 2,786,335 - Pf1N1B4_2456 0.38 +0.0 2,786,335 - Pf1N1B4_2456 0.38 -0.5 2,786,406 + Pf1N1B4_2456 0.60 +0.2 2,786,407 - Pf1N1B4_2456 0.60 +0.8 2,786,436 + Pf1N1B4_2456 0.69 +2.2 2,786,454 + Pf1N1B4_2456 0.75 -0.6 2,786,454 + Pf1N1B4_2456 0.75 +1.6 2,786,454 + Pf1N1B4_2456 0.75 +1.0 2,786,533 + -0.2 2,786,533 + -0.6 2,786,534 - +0.6 2,786,534 - +0.0 2,786,534 - +0.8 2,786,615 + +0.0 2,786,615 + +0.1 2,786,616 - -1.2 2,786,693 + -0.3 2,786,693 + -1.0 2,786,694 - +0.0 2,786,694 - -0.6 2,786,788 - +0.8 2,786,810 + -0.7 2,786,811 - +0.5 2,786,916 - Pf1N1B4_2457 0.13 +0.3 2,786,916 - Pf1N1B4_2457 0.13 +0.6 2,786,916 - Pf1N1B4_2457 0.13 -1.8 2,786,916 - Pf1N1B4_2457 0.13 +0.5 2,786,916 - Pf1N1B4_2457 0.13 -0.1 2,786,916 - Pf1N1B4_2457 0.13 -1.6 2,786,918 + Pf1N1B4_2457 0.13 -0.6 2,786,933 + +2.4 2,786,957 + Pf1N1B4_2457 0.14 -0.1 2,786,957 + Pf1N1B4_2457 0.14 +0.4 2,786,957 + Pf1N1B4_2457 0.14 -0.2 2,786,966 + Pf1N1B4_2457 0.14 -1.6 2,786,966 + Pf1N1B4_2457 0.14 +1.0 2,786,966 + Pf1N1B4_2457 0.14 +0.0 2,786,966 + Pf1N1B4_2457 0.14 +0.4 2,786,967 - Pf1N1B4_2457 0.14 +1.0 2,786,967 - Pf1N1B4_2457 0.14 +1.0 2,786,967 - Pf1N1B4_2457 0.14 +0.8 2,787,023 + Pf1N1B4_2457 0.16 +1.0 2,787,024 - Pf1N1B4_2457 0.16 +0.0 2,787,029 + Pf1N1B4_2457 0.16 +0.6 2,787,030 - -0.6 2,787,030 - Pf1N1B4_2457 0.16 +0.3 2,787,289 + Pf1N1B4_2457 0.25 -0.3 2,787,289 + Pf1N1B4_2457 0.25 -0.9 2,787,289 + Pf1N1B4_2457 0.25 -1.6 2,787,290 - Pf1N1B4_2457 0.25 -0.4 2,787,290 - Pf1N1B4_2457 0.25 +0.0 2,787,290 - Pf1N1B4_2457 0.25 +1.6 2,787,390 + Pf1N1B4_2457 0.28 -0.5 2,787,390 + Pf1N1B4_2457 0.28 +0.0 2,787,391 - Pf1N1B4_2457 0.28 +0.0 2,787,428 + Pf1N1B4_2457 0.30 -1.6 2,787,428 + Pf1N1B4_2457 0.30 -0.7 2,787,479 + Pf1N1B4_2457 0.31 -1.0 2,787,479 + Pf1N1B4_2457 0.31 +0.0 2,787,479 + Pf1N1B4_2457 0.31 +1.6 2,787,479 + Pf1N1B4_2457 0.31 +0.0 2,787,479 + Pf1N1B4_2457 0.31 +0.0 2,787,479 + Pf1N1B4_2457 0.31 +0.7 2,787,479 + Pf1N1B4_2457 0.31 +1.2 2,787,479 + Pf1N1B4_2457 0.31 +1.4 2,787,480 - Pf1N1B4_2457 0.31 +0.3 2,787,480 - Pf1N1B4_2457 0.31 +1.4 2,787,480 - Pf1N1B4_2457 0.31 +0.8 2,787,480 - Pf1N1B4_2457 0.31 +0.0 2,787,480 - Pf1N1B4_2457 0.31 -0.6 2,787,498 + Pf1N1B4_2457 0.32 +1.6
Or see this region's nucleotide sequence