Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04495 and H281DRAFT_04496 are separated by 2 nucleotides H281DRAFT_04496 and H281DRAFT_04497 are separated by 277 nucleotides H281DRAFT_04497 and H281DRAFT_04498 are separated by 80 nucleotides H281DRAFT_04498 and H281DRAFT_04499 are separated by 69 nucleotides
H281DRAFT_04495: H281DRAFT_04495 - 3-oxoacid CoA-transferase subunit A, at 227,919 to 228,623
_04495
H281DRAFT_04496: H281DRAFT_04496 - 3-oxoacid CoA-transferase subunit B, at 228,626 to 229,267
_04496
H281DRAFT_04497: H281DRAFT_04497 - Pimeloyl-ACP methyl ester carboxylesterase, at 229,545 to 230,456
_04497
H281DRAFT_04498: H281DRAFT_04498 - Enamine deaminase RidA, house cleaning of reactive enamine intermediates, YjgF/YER057c/UK114 family, at 230,537 to 230,890
_04498
H281DRAFT_04499: H281DRAFT_04499 - GTP pyrophosphokinase, at 230,960 to 233,203
_04499
Position (kb)
229
230
231 Strain fitness (log2 ratio)
-1
0
1
2 at 229.596 kb on + strand at 229.596 kb on + strand at 229.596 kb on + strand at 229.597 kb on - strand at 229.597 kb on - strand at 229.638 kb on + strand, within H281DRAFT_04497 at 229.638 kb on + strand, within H281DRAFT_04497 at 229.746 kb on + strand at 229.746 kb on + strand, within H281DRAFT_04497 at 229.746 kb on + strand, within H281DRAFT_04497 at 229.747 kb on - strand at 229.810 kb on + strand, within H281DRAFT_04497 at 229.810 kb on + strand, within H281DRAFT_04497 at 229.810 kb on + strand, within H281DRAFT_04497 at 229.810 kb on + strand, within H281DRAFT_04497 at 229.810 kb on + strand, within H281DRAFT_04497 at 229.810 kb on + strand, within H281DRAFT_04497 at 229.811 kb on - strand, within H281DRAFT_04497 at 229.811 kb on - strand, within H281DRAFT_04497 at 229.811 kb on - strand, within H281DRAFT_04497 at 229.827 kb on + strand, within H281DRAFT_04497 at 229.828 kb on - strand, within H281DRAFT_04497 at 229.828 kb on - strand, within H281DRAFT_04497 at 229.828 kb on - strand, within H281DRAFT_04497 at 229.961 kb on - strand, within H281DRAFT_04497 at 229.961 kb on - strand, within H281DRAFT_04497 at 230.124 kb on - strand, within H281DRAFT_04497 at 230.201 kb on + strand, within H281DRAFT_04497 at 230.236 kb on + strand, within H281DRAFT_04497 at 230.454 kb on + strand at 230.455 kb on - strand at 230.455 kb on - strand at 230.457 kb on + strand at 230.457 kb on + strand at 230.458 kb on - strand at 230.458 kb on - strand at 230.458 kb on - strand at 230.517 kb on + strand at 230.517 kb on + strand at 230.517 kb on + strand at 230.518 kb on - strand at 230.518 kb on - strand at 230.522 kb on - strand at 230.604 kb on - strand, within H281DRAFT_04498 at 230.707 kb on + strand, within H281DRAFT_04498 at 230.735 kb on + strand, within H281DRAFT_04498 at 230.736 kb on - strand, within H281DRAFT_04498 at 230.736 kb on - strand, within H281DRAFT_04498 at 230.736 kb on - strand, within H281DRAFT_04498 at 230.736 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 230.738 kb on - strand, within H281DRAFT_04498 at 231.384 kb on + strand, within H281DRAFT_04499 at 231.394 kb on - strand, within H281DRAFT_04499 at 231.429 kb on - strand, within H281DRAFT_04499
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 229,596 + -0.4 229,596 + +0.3 229,596 + +0.6 229,597 - +0.3 229,597 - +1.9 229,638 + H281DRAFT_04497 0.10 -1.1 229,638 + H281DRAFT_04497 0.10 +0.3 229,746 + -0.2 229,746 + H281DRAFT_04497 0.22 +1.8 229,746 + H281DRAFT_04497 0.22 -0.6 229,747 - +0.8 229,810 + H281DRAFT_04497 0.29 -0.4 229,810 + H281DRAFT_04497 0.29 -0.5 229,810 + H281DRAFT_04497 0.29 -0.9 229,810 + H281DRAFT_04497 0.29 -0.4 229,810 + H281DRAFT_04497 0.29 -0.1 229,810 + H281DRAFT_04497 0.29 -0.8 229,811 - H281DRAFT_04497 0.29 +0.1 229,811 - H281DRAFT_04497 0.29 +0.1 229,811 - H281DRAFT_04497 0.29 -0.4 229,827 + H281DRAFT_04497 0.31 -0.5 229,828 - H281DRAFT_04497 0.31 +0.4 229,828 - H281DRAFT_04497 0.31 +0.2 229,828 - H281DRAFT_04497 0.31 +0.5 229,961 - H281DRAFT_04497 0.46 +0.6 229,961 - H281DRAFT_04497 0.46 -0.2 230,124 - H281DRAFT_04497 0.63 -0.0 230,201 + H281DRAFT_04497 0.72 -0.2 230,236 + H281DRAFT_04497 0.76 -0.4 230,454 + -0.1 230,455 - -0.1 230,455 - +0.7 230,457 + +0.6 230,457 + -0.1 230,458 - +1.9 230,458 - +1.8 230,458 - +1.6 230,517 + -1.0 230,517 + +0.6 230,517 + -0.7 230,518 - +2.0 230,518 - +2.1 230,522 - +2.0 230,604 - H281DRAFT_04498 0.19 +2.1 230,707 + H281DRAFT_04498 0.48 -0.1 230,735 + H281DRAFT_04498 0.56 -0.2 230,736 - H281DRAFT_04498 0.56 +1.2 230,736 - H281DRAFT_04498 0.56 +1.5 230,736 - H281DRAFT_04498 0.56 +1.5 230,736 - H281DRAFT_04498 0.56 +1.3 230,738 - H281DRAFT_04498 0.57 +1.6 230,738 - H281DRAFT_04498 0.57 +2.5 230,738 - H281DRAFT_04498 0.57 +1.3 230,738 - H281DRAFT_04498 0.57 +1.5 230,738 - H281DRAFT_04498 0.57 +2.0 231,384 + H281DRAFT_04499 0.19 +2.0 231,394 - H281DRAFT_04499 0.19 +1.9 231,429 - H281DRAFT_04499 0.21 +1.3
Or see this region's nucleotide sequence