Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04355 and H281DRAFT_04356 are separated by 83 nucleotides H281DRAFT_04356 and H281DRAFT_04357 are separated by 4 nucleotides H281DRAFT_04357 and H281DRAFT_04358 are separated by 35 nucleotides
H281DRAFT_04355: H281DRAFT_04355 - RNA polymerase, sigma-24 subunit, RpoE, at 72,044 to 72,643
_04355
H281DRAFT_04356: H281DRAFT_04356 - anti sigma-E protein, RseA, at 72,727 to 73,341
_04356
H281DRAFT_04357: H281DRAFT_04357 - sigma E regulatory protein, MucB/RseB, at 73,346 to 74,389
_04357
H281DRAFT_04358: H281DRAFT_04358 - serine protease Do, at 74,425 to 75,951
_04358
Position (kb)
73
74
75 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 72.677 kb on + strand at 72.677 kb on + strand at 72.686 kb on + strand at 72.686 kb on + strand at 72.711 kb on + strand at 72.711 kb on + strand at 72.711 kb on + strand at 72.712 kb on - strand at 72.712 kb on - strand at 72.878 kb on - strand, within H281DRAFT_04356 at 73.084 kb on - strand, within H281DRAFT_04356 at 73.247 kb on - strand, within H281DRAFT_04356 at 73.339 kb on + strand at 73.339 kb on + strand at 73.340 kb on - strand at 73.340 kb on - strand at 73.369 kb on - strand at 73.369 kb on - strand at 73.376 kb on - strand at 73.429 kb on + strand at 73.547 kb on + strand, within H281DRAFT_04357 at 73.589 kb on + strand, within H281DRAFT_04357 at 73.678 kb on + strand, within H281DRAFT_04357 at 73.678 kb on + strand, within H281DRAFT_04357 at 73.781 kb on + strand, within H281DRAFT_04357 at 73.781 kb on + strand, within H281DRAFT_04357 at 73.872 kb on - strand, within H281DRAFT_04357 at 74.012 kb on - strand, within H281DRAFT_04357 at 74.283 kb on + strand, within H281DRAFT_04357 at 74.283 kb on + strand, within H281DRAFT_04357 at 74.283 kb on + strand, within H281DRAFT_04357 at 74.283 kb on + strand, within H281DRAFT_04357 at 74.284 kb on - strand, within H281DRAFT_04357 at 74.284 kb on - strand, within H281DRAFT_04357 at 74.284 kb on - strand, within H281DRAFT_04357 at 74.286 kb on + strand at 74.315 kb on - strand at 74.367 kb on + strand at 74.367 kb on + strand at 74.368 kb on - strand at 74.368 kb on - strand at 74.372 kb on + strand at 74.372 kb on + strand at 74.372 kb on + strand at 74.373 kb on - strand at 74.373 kb on - strand at 74.388 kb on - strand at 74.842 kb on + strand, within H281DRAFT_04358 at 74.843 kb on - strand, within H281DRAFT_04358 at 74.887 kb on + strand, within H281DRAFT_04358 at 74.887 kb on + strand, within H281DRAFT_04358 at 74.887 kb on + strand, within H281DRAFT_04358 at 74.887 kb on + strand, within H281DRAFT_04358 at 74.888 kb on - strand, within H281DRAFT_04358 at 75.144 kb on - strand, within H281DRAFT_04358 at 75.197 kb on + strand, within H281DRAFT_04358 at 75.197 kb on + strand, within H281DRAFT_04358 at 75.220 kb on + strand, within H281DRAFT_04358 at 75.220 kb on + strand, within H281DRAFT_04358 at 75.220 kb on + strand, within H281DRAFT_04358 at 75.240 kb on + strand, within H281DRAFT_04358 at 75.241 kb on - strand, within H281DRAFT_04358
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 72,677 + -0.1 72,677 + +1.6 72,686 + +0.2 72,686 + -2.5 72,711 + +0.8 72,711 + -0.5 72,711 + +0.8 72,712 - -1.8 72,712 - -1.8 72,878 - H281DRAFT_04356 0.25 +0.8 73,084 - H281DRAFT_04356 0.58 -1.2 73,247 - H281DRAFT_04356 0.85 -1.1 73,339 + -2.9 73,339 + -0.8 73,340 - -1.4 73,340 - -1.4 73,369 - -1.4 73,369 - -0.9 73,376 - -1.0 73,429 + -1.4 73,547 + H281DRAFT_04357 0.19 -1.4 73,589 + H281DRAFT_04357 0.23 -2.9 73,678 + H281DRAFT_04357 0.32 +0.8 73,678 + H281DRAFT_04357 0.32 -0.2 73,781 + H281DRAFT_04357 0.42 -1.2 73,781 + H281DRAFT_04357 0.42 -0.8 73,872 - H281DRAFT_04357 0.50 -0.2 74,012 - H281DRAFT_04357 0.64 +1.1 74,283 + H281DRAFT_04357 0.90 -1.4 74,283 + H281DRAFT_04357 0.90 -1.3 74,283 + H281DRAFT_04357 0.90 -1.4 74,283 + H281DRAFT_04357 0.90 -1.5 74,284 - H281DRAFT_04357 0.90 -1.4 74,284 - H281DRAFT_04357 0.90 -2.4 74,284 - H281DRAFT_04357 0.90 -2.0 74,286 + -1.2 74,315 - -0.5 74,367 + -2.4 74,367 + +0.8 74,368 - -1.6 74,368 - -1.5 74,372 + -0.6 74,372 + +0.7 74,372 + +1.7 74,373 - -0.8 74,373 - +0.2 74,388 - -1.5 74,842 + H281DRAFT_04358 0.27 -0.4 74,843 - H281DRAFT_04358 0.27 +1.1 74,887 + H281DRAFT_04358 0.30 +0.8 74,887 + H281DRAFT_04358 0.30 +0.2 74,887 + H281DRAFT_04358 0.30 +0.3 74,887 + H281DRAFT_04358 0.30 -1.5 74,888 - H281DRAFT_04358 0.30 +0.4 75,144 - H281DRAFT_04358 0.47 +0.4 75,197 + H281DRAFT_04358 0.51 -0.6 75,197 + H281DRAFT_04358 0.51 +0.5 75,220 + H281DRAFT_04358 0.52 +0.8 75,220 + H281DRAFT_04358 0.52 -0.7 75,220 + H281DRAFT_04358 0.52 +0.0 75,240 + H281DRAFT_04358 0.53 -0.4 75,241 - H281DRAFT_04358 0.53 -0.5
Or see this region's nucleotide sequence