Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_04321

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_04319 and H281DRAFT_04320 are separated by 81 nucleotidesH281DRAFT_04320 and H281DRAFT_04321 are separated by 218 nucleotidesH281DRAFT_04321 and H281DRAFT_04322 are separated by 151 nucleotides H281DRAFT_04319: H281DRAFT_04319 - hypothetical protein, at 37,549 to 38,013 _04319 H281DRAFT_04320: H281DRAFT_04320 - hypothetical protein, at 38,095 to 38,376 _04320 H281DRAFT_04321: H281DRAFT_04321 - DNA helicase-2 / ATP-dependent DNA helicase PcrA, at 38,595 to 40,724 _04321 H281DRAFT_04322: H281DRAFT_04322 - Protein of unknown function (DUF4088), at 40,876 to 41,643 _04322 Position (kb) 38 39 40 41Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 37.656 kb on + strand, within H281DRAFT_04319at 37.657 kb on - strand, within H281DRAFT_04319at 37.664 kb on + strand, within H281DRAFT_04319at 37.664 kb on + strand, within H281DRAFT_04319at 37.664 kb on + strand, within H281DRAFT_04319at 37.665 kb on - strand, within H281DRAFT_04319at 37.665 kb on - strand, within H281DRAFT_04319at 37.665 kb on - strand, within H281DRAFT_04319at 37.665 kb on - strand, within H281DRAFT_04319at 37.782 kb on - strand, within H281DRAFT_04319at 37.782 kb on - strand, within H281DRAFT_04319at 37.885 kb on + strand, within H281DRAFT_04319at 37.886 kb on - strand, within H281DRAFT_04319at 37.886 kb on - strand, within H281DRAFT_04319at 37.886 kb on - strand, within H281DRAFT_04319at 37.886 kb on - strand, within H281DRAFT_04319at 38.004 kb on + strandat 38.004 kb on + strandat 38.081 kb on + strandat 38.112 kb on + strandat 38.374 kb on + strandat 38.375 kb on - strandat 38.375 kb on - strandat 38.375 kb on - strandat 38.432 kb on + strandat 38.466 kb on + strandat 38.467 kb on - strandat 38.510 kb on + strandat 38.547 kb on + strandat 38.547 kb on + strandat 38.548 kb on - strandat 38.586 kb on + strandat 38.586 kb on + strandat 38.774 kb on - strandat 38.866 kb on + strand, within H281DRAFT_04321at 38.867 kb on - strand, within H281DRAFT_04321at 38.867 kb on - strand, within H281DRAFT_04321at 38.870 kb on + strand, within H281DRAFT_04321at 38.870 kb on + strand, within H281DRAFT_04321at 38.870 kb on + strand, within H281DRAFT_04321at 38.870 kb on + strand, within H281DRAFT_04321at 38.870 kb on + strand, within H281DRAFT_04321at 38.870 kb on + strand, within H281DRAFT_04321at 38.870 kb on + strand, within H281DRAFT_04321at 38.871 kb on - strand, within H281DRAFT_04321at 38.871 kb on - strand, within H281DRAFT_04321at 38.871 kb on - strand, within H281DRAFT_04321at 38.939 kb on + strand, within H281DRAFT_04321at 38.967 kb on + strand, within H281DRAFT_04321at 38.968 kb on - strand, within H281DRAFT_04321at 39.216 kb on + strand, within H281DRAFT_04321at 39.270 kb on + strand, within H281DRAFT_04321at 39.271 kb on - strand, within H281DRAFT_04321at 39.348 kb on + strand, within H281DRAFT_04321at 39.349 kb on - strand, within H281DRAFT_04321at 39.349 kb on - strandat 39.444 kb on + strand, within H281DRAFT_04321at 39.445 kb on - strandat 39.534 kb on + strand, within H281DRAFT_04321at 39.535 kb on - strand, within H281DRAFT_04321at 39.599 kb on + strand, within H281DRAFT_04321at 39.599 kb on + strand, within H281DRAFT_04321at 39.600 kb on - strand, within H281DRAFT_04321at 39.681 kb on + strand, within H281DRAFT_04321at 39.681 kb on + strand, within H281DRAFT_04321at 39.684 kb on - strand, within H281DRAFT_04321at 39.860 kb on - strand, within H281DRAFT_04321at 39.920 kb on - strand, within H281DRAFT_04321at 39.926 kb on - strand, within H281DRAFT_04321at 40.095 kb on + strand, within H281DRAFT_04321at 40.096 kb on - strand, within H281DRAFT_04321at 40.096 kb on - strand, within H281DRAFT_04321at 40.147 kb on - strand, within H281DRAFT_04321at 40.230 kb on + strand, within H281DRAFT_04321at 40.231 kb on - strand, within H281DRAFT_04321at 40.231 kb on - strand, within H281DRAFT_04321at 40.231 kb on - strand, within H281DRAFT_04321at 40.231 kb on - strand, within H281DRAFT_04321at 40.324 kb on - strand, within H281DRAFT_04321at 40.464 kb on + strand, within H281DRAFT_04321at 40.500 kb on - strand, within H281DRAFT_04321at 40.500 kb on - strand, within H281DRAFT_04321at 40.542 kb on - strandat 40.542 kb on - strandat 40.542 kb on - strandat 40.614 kb on + strandat 40.615 kb on - strandat 40.615 kb on - strandat 40.615 kb on - strandat 40.837 kb on - strandat 40.843 kb on + strandat 40.843 kb on + strandat 40.844 kb on - strandat 40.844 kb on - strandat 40.967 kb on + strand, within H281DRAFT_04322at 41.009 kb on + strand, within H281DRAFT_04322at 41.010 kb on - strand, within H281DRAFT_04322at 41.144 kb on + strand, within H281DRAFT_04322at 41.145 kb on - strand, within H281DRAFT_04322at 41.442 kb on - strand, within H281DRAFT_04322at 41.442 kb on - strand, within H281DRAFT_04322at 41.442 kb on - strand, within H281DRAFT_04322at 41.631 kb on + strandat 41.631 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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37,656 + H281DRAFT_04319 0.23 +0.2
37,657 - H281DRAFT_04319 0.23 +0.1
37,664 + H281DRAFT_04319 0.25 +0.4
37,664 + H281DRAFT_04319 0.25 +0.3
37,664 + H281DRAFT_04319 0.25 -3.4
37,665 - H281DRAFT_04319 0.25 -0.3
37,665 - H281DRAFT_04319 0.25 -0.9
37,665 - H281DRAFT_04319 0.25 -0.9
37,665 - H281DRAFT_04319 0.25 +0.3
37,782 - H281DRAFT_04319 0.50 +0.6
37,782 - H281DRAFT_04319 0.50 -0.4
37,885 + H281DRAFT_04319 0.72 +0.1
37,886 - H281DRAFT_04319 0.72 +0.5
37,886 - H281DRAFT_04319 0.72 +1.0
37,886 - H281DRAFT_04319 0.72 +0.7
37,886 - H281DRAFT_04319 0.72 +0.3
38,004 + +0.6
38,004 + +0.2
38,081 + -0.3
38,112 + +1.0
38,374 + -0.2
38,375 - +0.5
38,375 - -0.7
38,375 - +0.6
38,432 + -0.6
38,466 + -0.3
38,467 - +0.6
38,510 + +0.8
38,547 + +0.1
38,547 + -0.1
38,548 - +1.8
38,586 + -0.7
38,586 + +2.8
38,774 - +0.1
38,866 + H281DRAFT_04321 0.13 +0.3
38,867 - H281DRAFT_04321 0.13 -0.2
38,867 - H281DRAFT_04321 0.13 -0.4
38,870 + H281DRAFT_04321 0.13 -1.0
38,870 + H281DRAFT_04321 0.13 -0.3
38,870 + H281DRAFT_04321 0.13 -0.4
38,870 + H281DRAFT_04321 0.13 -0.2
38,870 + H281DRAFT_04321 0.13 -1.0
38,870 + H281DRAFT_04321 0.13 +0.6
38,870 + H281DRAFT_04321 0.13 +0.3
38,871 - H281DRAFT_04321 0.13 -0.5
38,871 - H281DRAFT_04321 0.13 +0.1
38,871 - H281DRAFT_04321 0.13 +0.5
38,939 + H281DRAFT_04321 0.16 +1.3
38,967 + H281DRAFT_04321 0.17 +2.3
38,968 - H281DRAFT_04321 0.18 -0.0
39,216 + H281DRAFT_04321 0.29 -0.8
39,270 + H281DRAFT_04321 0.32 -0.4
39,271 - H281DRAFT_04321 0.32 -0.3
39,348 + H281DRAFT_04321 0.35 +0.8
39,349 - H281DRAFT_04321 0.35 -0.1
39,349 - -1.2
39,444 + H281DRAFT_04321 0.40 +1.4
39,445 - -0.8
39,534 + H281DRAFT_04321 0.44 +0.4
39,535 - H281DRAFT_04321 0.44 +0.1
39,599 + H281DRAFT_04321 0.47 +0.1
39,599 + H281DRAFT_04321 0.47 +0.5
39,600 - H281DRAFT_04321 0.47 +1.9
39,681 + H281DRAFT_04321 0.51 +1.8
39,681 + H281DRAFT_04321 0.51 +0.3
39,684 - H281DRAFT_04321 0.51 +1.2
39,860 - H281DRAFT_04321 0.59 -0.3
39,920 - H281DRAFT_04321 0.62 +0.2
39,926 - H281DRAFT_04321 0.62 -0.2
40,095 + H281DRAFT_04321 0.70 +0.3
40,096 - H281DRAFT_04321 0.70 +1.7
40,096 - H281DRAFT_04321 0.70 -0.4
40,147 - H281DRAFT_04321 0.73 -0.4
40,230 + H281DRAFT_04321 0.77 +0.5
40,231 - H281DRAFT_04321 0.77 -0.4
40,231 - H281DRAFT_04321 0.77 -0.8
40,231 - H281DRAFT_04321 0.77 -1.1
40,231 - H281DRAFT_04321 0.77 +0.5
40,324 - H281DRAFT_04321 0.81 -0.0
40,464 + H281DRAFT_04321 0.88 +0.7
40,500 - H281DRAFT_04321 0.89 +1.2
40,500 - H281DRAFT_04321 0.89 +0.3
40,542 - +0.6
40,542 - -0.6
40,542 - -2.2
40,614 + +0.1
40,615 - -1.3
40,615 - +0.1
40,615 - -1.3
40,837 - -0.8
40,843 + +0.0
40,843 + -0.5
40,844 - +0.8
40,844 - +1.1
40,967 + H281DRAFT_04322 0.12 -3.5
41,009 + H281DRAFT_04322 0.17 -0.9
41,010 - H281DRAFT_04322 0.17 +0.1
41,144 + H281DRAFT_04322 0.35 +0.2
41,145 - H281DRAFT_04322 0.35 -0.5
41,442 - H281DRAFT_04322 0.74 -0.6
41,442 - H281DRAFT_04322 0.74 +0.3
41,442 - H281DRAFT_04322 0.74 -2.2
41,631 + -0.2
41,631 + -0.7

Or see this region's nucleotide sequence