Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04268 and H281DRAFT_04269 overlap by 1 nucleotides H281DRAFT_04269 and H281DRAFT_04270 are separated by 82 nucleotides H281DRAFT_04270 and H281DRAFT_04271 are separated by 285 nucleotides
H281DRAFT_04268: H281DRAFT_04268 - L-glutamate ABC transporter membrane protein /L-aspartate ABC transporter membrane protein, at 386,091 to 386,768
_04268
H281DRAFT_04269: H281DRAFT_04269 - L-glutamate ABC transporter membrane protein /L-aspartate ABC transporter membrane protein, at 386,768 to 387,508
_04269
H281DRAFT_04270: H281DRAFT_04270 - L-glutamate-binding protein /L-aspartate-binding protein, at 387,591 to 388,484
_04270
H281DRAFT_04271: H281DRAFT_04271 - glutamate dehydrogenase (NAD(P)+), at 388,770 to 390,083
_04271
Position (kb) 387
388
389 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 386.593 kb on + strand, within H281DRAFT_04268 at 386.593 kb on + strand, within H281DRAFT_04268 at 386.594 kb on - strand, within H281DRAFT_04268 at 386.594 kb on - strand, within H281DRAFT_04268 at 386.782 kb on + strand at 386.784 kb on + strand at 386.853 kb on + strand, within H281DRAFT_04269 at 386.883 kb on + strand, within H281DRAFT_04269 at 386.883 kb on + strand, within H281DRAFT_04269 at 386.884 kb on - strand, within H281DRAFT_04269 at 386.899 kb on - strand, within H281DRAFT_04269 at 386.974 kb on + strand, within H281DRAFT_04269 at 386.974 kb on + strand, within H281DRAFT_04269 at 386.974 kb on + strand, within H281DRAFT_04269 at 386.975 kb on - strand, within H281DRAFT_04269 at 386.975 kb on - strand, within H281DRAFT_04269 at 387.036 kb on + strand, within H281DRAFT_04269 at 387.036 kb on + strand, within H281DRAFT_04269 at 387.243 kb on - strand, within H281DRAFT_04269 at 387.252 kb on + strand, within H281DRAFT_04269 at 387.253 kb on - strand, within H281DRAFT_04269 at 387.253 kb on - strand, within H281DRAFT_04269 at 387.304 kb on - strand, within H281DRAFT_04269 at 387.304 kb on - strand, within H281DRAFT_04269 at 387.444 kb on + strand at 387.444 kb on + strand at 387.445 kb on - strand at 387.445 kb on - strand at 387.501 kb on + strand at 387.502 kb on - strand at 387.502 kb on - strand at 387.502 kb on - strand at 387.585 kb on + strand at 387.585 kb on + strand at 387.586 kb on - strand at 387.586 kb on - strand at 387.592 kb on + strand at 387.704 kb on - strand, within H281DRAFT_04270 at 387.704 kb on - strand, within H281DRAFT_04270 at 387.799 kb on + strand, within H281DRAFT_04270 at 387.800 kb on - strand, within H281DRAFT_04270 at 388.151 kb on + strand, within H281DRAFT_04270 at 388.206 kb on - strand, within H281DRAFT_04270 at 388.333 kb on + strand, within H281DRAFT_04270 at 388.333 kb on + strand, within H281DRAFT_04270 at 388.337 kb on - strand, within H281DRAFT_04270 at 388.376 kb on + strand, within H281DRAFT_04270 at 388.407 kb on - strand at 388.518 kb on + strand at 388.526 kb on + strand at 388.530 kb on + strand at 388.530 kb on + strand at 388.541 kb on + strand at 388.541 kb on + strand at 388.547 kb on + strand at 388.548 kb on - strand at 388.548 kb on - strand at 388.549 kb on + strand at 388.550 kb on - strand at 388.550 kb on - strand at 388.602 kb on + strand at 388.620 kb on + strand at 388.623 kb on - strand at 388.623 kb on - strand at 388.623 kb on - strand at 388.623 kb on - strand at 388.623 kb on - strand at 388.623 kb on - strand at 388.733 kb on + strand at 388.734 kb on - strand at 388.758 kb on + strand at 388.758 kb on + strand at 388.759 kb on - strand at 388.759 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 386,593 + H281DRAFT_04268 0.74 -1.1 386,593 + H281DRAFT_04268 0.74 -1.5 386,594 - H281DRAFT_04268 0.74 -1.3 386,594 - H281DRAFT_04268 0.74 -0.7 386,782 + -0.7 386,784 + +0.4 386,853 + H281DRAFT_04269 0.11 -1.0 386,883 + H281DRAFT_04269 0.16 -2.5 386,883 + H281DRAFT_04269 0.16 -0.5 386,884 - H281DRAFT_04269 0.16 -0.3 386,899 - H281DRAFT_04269 0.18 -2.6 386,974 + H281DRAFT_04269 0.28 -2.8 386,974 + H281DRAFT_04269 0.28 +0.5 386,974 + H281DRAFT_04269 0.28 -0.9 386,975 - H281DRAFT_04269 0.28 -3.4 386,975 - H281DRAFT_04269 0.28 +0.1 387,036 + H281DRAFT_04269 0.36 -3.8 387,036 + H281DRAFT_04269 0.36 -1.5 387,243 - H281DRAFT_04269 0.64 +1.4 387,252 + H281DRAFT_04269 0.65 -2.3 387,253 - H281DRAFT_04269 0.65 -0.6 387,253 - H281DRAFT_04269 0.65 -1.3 387,304 - H281DRAFT_04269 0.72 -1.3 387,304 - H281DRAFT_04269 0.72 -1.6 387,444 + -1.0 387,444 + -2.3 387,445 - -1.3 387,445 - -0.6 387,501 + -1.1 387,502 - -1.1 387,502 - -2.2 387,502 - -2.2 387,585 + -1.4 387,585 + -0.8 387,586 - +1.0 387,586 - -0.1 387,592 + -0.8 387,704 - H281DRAFT_04270 0.13 +0.9 387,704 - H281DRAFT_04270 0.13 +0.5 387,799 + H281DRAFT_04270 0.23 +0.7 387,800 - H281DRAFT_04270 0.23 -0.0 388,151 + H281DRAFT_04270 0.63 +0.4 388,206 - H281DRAFT_04270 0.69 +1.1 388,333 + H281DRAFT_04270 0.83 +0.9 388,333 + H281DRAFT_04270 0.83 +0.7 388,337 - H281DRAFT_04270 0.83 +0.5 388,376 + H281DRAFT_04270 0.88 -0.1 388,407 - +0.4 388,518 + -1.6 388,526 + -0.7 388,530 + +0.2 388,530 + +0.7 388,541 + -0.7 388,541 + -0.3 388,547 + -0.2 388,548 - +0.0 388,548 - +0.4 388,549 + -0.3 388,550 - -0.1 388,550 - -1.1 388,602 + +0.1 388,620 + +1.3 388,623 - -1.5 388,623 - +0.4 388,623 - +0.4 388,623 - +0.9 388,623 - -2.6 388,623 - -0.7 388,733 + -0.2 388,734 - -0.2 388,758 + +1.4 388,758 + +1.8 388,759 - -0.7 388,759 - +0.5
Or see this region's nucleotide sequence