Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_03613

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_03612 and H281DRAFT_03613 are separated by 2 nucleotidesH281DRAFT_03613 and H281DRAFT_03614 are separated by 223 nucleotidesH281DRAFT_03614 and H281DRAFT_03615 are separated by 142 nucleotides H281DRAFT_03612: H281DRAFT_03612 - phospholipid-binding lipoprotein MlaA, at 121,757 to 122,827 _03612 H281DRAFT_03613: H281DRAFT_03613 - hypothetical protein, at 122,830 to 125,445 _03613 H281DRAFT_03614: H281DRAFT_03614 - ABC-type transporter Mla maintaining outer membrane lipid asymmetry, MlaC component, at 125,669 to 126,274 _03614 H281DRAFT_03615: H281DRAFT_03615 - hopanoid biosynthesis associated radical SAM protein HpnH, at 126,417 to 127,571 _03615 Position (kb) 122 123 124 125 126Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 121.888 kb on + strand, within H281DRAFT_03612at 122.047 kb on - strand, within H281DRAFT_03612at 122.189 kb on - strand, within H281DRAFT_03612at 122.190 kb on + strand, within H281DRAFT_03612at 122.190 kb on + strand, within H281DRAFT_03612at 122.191 kb on - strand, within H281DRAFT_03612at 122.191 kb on - strand, within H281DRAFT_03612at 122.211 kb on + strand, within H281DRAFT_03612at 122.211 kb on + strand, within H281DRAFT_03612at 122.211 kb on + strand, within H281DRAFT_03612at 122.215 kb on + strand, within H281DRAFT_03612at 122.319 kb on + strand, within H281DRAFT_03612at 122.319 kb on + strand, within H281DRAFT_03612at 122.320 kb on - strand, within H281DRAFT_03612at 122.320 kb on - strand, within H281DRAFT_03612at 122.320 kb on - strand, within H281DRAFT_03612at 122.320 kb on - strand, within H281DRAFT_03612at 122.320 kb on - strand, within H281DRAFT_03612at 122.404 kb on - strand, within H281DRAFT_03612at 122.658 kb on + strand, within H281DRAFT_03612at 122.686 kb on - strand, within H281DRAFT_03612at 122.816 kb on - strandat 122.816 kb on - strandat 122.831 kb on + strandat 122.831 kb on + strandat 122.832 kb on - strandat 122.832 kb on - strandat 122.832 kb on - strandat 122.832 kb on - strandat 122.834 kb on - strandat 122.834 kb on - strandat 122.834 kb on - strandat 122.834 kb on - strandat 123.080 kb on + strandat 123.081 kb on - strandat 123.129 kb on + strand, within H281DRAFT_03613at 123.130 kb on - strandat 123.309 kb on + strand, within H281DRAFT_03613at 124.263 kb on - strand, within H281DRAFT_03613at 124.381 kb on - strand, within H281DRAFT_03613at 124.395 kb on - strand, within H281DRAFT_03613at 124.763 kb on - strand, within H281DRAFT_03613at 124.763 kb on - strand, within H281DRAFT_03613at 124.876 kb on + strandat 124.885 kb on + strand, within H281DRAFT_03613at 124.969 kb on - strand, within H281DRAFT_03613at 125.095 kb on - strand, within H281DRAFT_03613at 125.345 kb on + strandat 125.346 kb on - strandat 125.346 kb on - strandat 125.430 kb on + strandat 125.430 kb on + strandat 125.430 kb on + strandat 125.430 kb on + strandat 125.431 kb on - strandat 125.431 kb on - strandat 125.431 kb on - strandat 125.431 kb on - strandat 125.447 kb on + strandat 125.464 kb on - strandat 125.497 kb on + strandat 125.497 kb on + strandat 125.497 kb on + strandat 125.497 kb on + strandat 125.497 kb on + strandat 125.498 kb on - strandat 125.498 kb on - strandat 125.498 kb on - strandat 125.499 kb on + strandat 125.594 kb on + strandat 125.595 kb on - strandat 125.595 kb on - strandat 125.596 kb on + strandat 125.596 kb on + strandat 125.597 kb on - strandat 125.597 kb on - strandat 125.668 kb on + strandat 125.668 kb on + strandat 125.668 kb on + strandat 125.678 kb on + strandat 125.706 kb on + strandat 125.751 kb on + strand, within H281DRAFT_03614at 125.752 kb on - strand, within H281DRAFT_03614at 125.770 kb on + strand, within H281DRAFT_03614at 125.939 kb on + strand, within H281DRAFT_03614at 125.939 kb on + strand, within H281DRAFT_03614at 125.939 kb on + strand, within H281DRAFT_03614at 125.940 kb on - strand, within H281DRAFT_03614at 125.940 kb on - strand, within H281DRAFT_03614at 125.940 kb on - strand, within H281DRAFT_03614at 125.972 kb on + strand, within H281DRAFT_03614at 125.972 kb on + strand, within H281DRAFT_03614at 125.973 kb on - strand, within H281DRAFT_03614at 126.143 kb on + strand, within H281DRAFT_03614at 126.143 kb on + strand, within H281DRAFT_03614at 126.143 kb on + strand, within H281DRAFT_03614at 126.144 kb on - strand, within H281DRAFT_03614at 126.144 kb on - strand, within H281DRAFT_03614at 126.154 kb on + strand, within H281DRAFT_03614at 126.154 kb on + strand, within H281DRAFT_03614at 126.155 kb on - strand, within H281DRAFT_03614at 126.155 kb on - strand, within H281DRAFT_03614at 126.183 kb on - strand, within H281DRAFT_03614at 126.305 kb on + strandat 126.305 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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121,888 + H281DRAFT_03612 0.12 +0.3
122,047 - H281DRAFT_03612 0.27 +0.4
122,189 - H281DRAFT_03612 0.40 +0.4
122,190 + H281DRAFT_03612 0.40 +0.5
122,190 + H281DRAFT_03612 0.40 +0.6
122,191 - H281DRAFT_03612 0.41 +1.2
122,191 - H281DRAFT_03612 0.41 +1.1
122,211 + H281DRAFT_03612 0.42 +0.4
122,211 + H281DRAFT_03612 0.42 +1.3
122,211 + H281DRAFT_03612 0.42 +0.3
122,215 + H281DRAFT_03612 0.43 -0.4
122,319 + H281DRAFT_03612 0.52 -0.0
122,319 + H281DRAFT_03612 0.52 +0.9
122,320 - H281DRAFT_03612 0.53 +1.0
122,320 - H281DRAFT_03612 0.53 +0.8
122,320 - H281DRAFT_03612 0.53 +0.7
122,320 - H281DRAFT_03612 0.53 +0.4
122,320 - H281DRAFT_03612 0.53 +0.0
122,404 - H281DRAFT_03612 0.60 +0.3
122,658 + H281DRAFT_03612 0.84 -1.0
122,686 - H281DRAFT_03612 0.87 +0.9
122,816 - +0.5
122,816 - +1.1
122,831 + -0.0
122,831 + +0.4
122,832 - +0.2
122,832 - +0.6
122,832 - -0.1
122,832 - +0.4
122,834 - +0.6
122,834 - -4.0
122,834 - +0.3
122,834 - -0.3
123,080 + -1.8
123,081 - -0.2
123,129 + H281DRAFT_03613 0.11 -0.0
123,130 - +0.5
123,309 + H281DRAFT_03613 0.18 -0.7
124,263 - H281DRAFT_03613 0.55 +0.2
124,381 - H281DRAFT_03613 0.59 +0.8
124,395 - H281DRAFT_03613 0.60 +0.7
124,763 - H281DRAFT_03613 0.74 +1.1
124,763 - H281DRAFT_03613 0.74 +1.1
124,876 + -1.7
124,885 + H281DRAFT_03613 0.79 -1.0
124,969 - H281DRAFT_03613 0.82 +1.1
125,095 - H281DRAFT_03613 0.87 -0.9
125,345 + -1.8
125,346 - -0.1
125,346 - +0.7
125,430 + +0.8
125,430 + +0.9
125,430 + -0.4
125,430 + -1.2
125,431 - -0.6
125,431 - +0.5
125,431 - -0.1
125,431 - +0.7
125,447 + +0.6
125,464 - +0.1
125,497 + +0.3
125,497 + -1.0
125,497 + +0.2
125,497 + +0.6
125,497 + -1.0
125,498 - +0.3
125,498 - +0.6
125,498 - +0.1
125,499 + -0.8
125,594 + -1.0
125,595 - +0.3
125,595 - -2.6
125,596 + +0.1
125,596 + +0.9
125,597 - +0.3
125,597 - +2.1
125,668 + -1.5
125,668 + -0.6
125,668 + -0.7
125,678 + +1.2
125,706 + +0.5
125,751 + H281DRAFT_03614 0.14 +3.8
125,752 - H281DRAFT_03614 0.14 -0.1
125,770 + H281DRAFT_03614 0.17 -0.1
125,939 + H281DRAFT_03614 0.45 -0.1
125,939 + H281DRAFT_03614 0.45 -0.8
125,939 + H281DRAFT_03614 0.45 +0.9
125,940 - H281DRAFT_03614 0.45 -0.5
125,940 - H281DRAFT_03614 0.45 +0.1
125,940 - H281DRAFT_03614 0.45 +1.4
125,972 + H281DRAFT_03614 0.50 -0.2
125,972 + H281DRAFT_03614 0.50 +0.3
125,973 - H281DRAFT_03614 0.50 -0.6
126,143 + H281DRAFT_03614 0.78 +1.8
126,143 + H281DRAFT_03614 0.78 -0.5
126,143 + H281DRAFT_03614 0.78 +0.2
126,144 - H281DRAFT_03614 0.78 +0.0
126,144 - H281DRAFT_03614 0.78 +0.0
126,154 + H281DRAFT_03614 0.80 -0.0
126,154 + H281DRAFT_03614 0.80 +1.5
126,155 - H281DRAFT_03614 0.80 +0.6
126,155 - H281DRAFT_03614 0.80 +0.4
126,183 - H281DRAFT_03614 0.85 +0.4
126,305 + +0.6
126,305 + -1.4

Or see this region's nucleotide sequence