Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03518 and H281DRAFT_03519 are separated by 439 nucleotides H281DRAFT_03519 and H281DRAFT_03520 are separated by 370 nucleotides H281DRAFT_03520 and H281DRAFT_03521 are separated by 129 nucleotides H281DRAFT_03521 and H281DRAFT_03522 are separated by 164 nucleotides
H281DRAFT_03518: H281DRAFT_03518 - hypothetical protein, at 18,611 to 18,916
_03518
H281DRAFT_03519: H281DRAFT_03519 - hypothetical protein, at 19,356 to 19,514
_03519
H281DRAFT_03520: H281DRAFT_03520 - hypothetical protein, at 19,885 to 21,225
_03520
H281DRAFT_03521: H281DRAFT_03521 - hypothetical protein, at 21,355 to 21,585
_03521
H281DRAFT_03522: H281DRAFT_03522 - Uncharacterized membrane protein, at 21,750 to 22,040
_03522
Position (kb) 19
20
21
22 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 18.916 kb on + strand at 18.916 kb on + strand at 18.916 kb on + strand at 18.917 kb on - strand at 18.917 kb on - strand at 18.917 kb on - strand at 18.917 kb on - strand at 19.090 kb on - strand at 19.172 kb on - strand at 19.193 kb on + strand at 19.224 kb on + strand at 19.225 kb on - strand at 19.225 kb on - strand at 19.225 kb on - strand at 19.226 kb on + strand at 19.226 kb on + strand at 19.226 kb on + strand at 19.227 kb on - strand at 19.354 kb on + strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.355 kb on - strand at 19.357 kb on + strand at 19.357 kb on + strand at 19.432 kb on + strand, within H281DRAFT_03519 at 19.438 kb on + strand, within H281DRAFT_03519 at 19.439 kb on - strand, within H281DRAFT_03519 at 19.531 kb on + strand at 19.532 kb on - strand at 19.607 kb on + strand at 19.607 kb on + strand at 19.607 kb on + strand at 19.607 kb on + strand at 19.608 kb on - strand at 19.608 kb on - strand at 19.664 kb on - strand at 19.664 kb on - strand at 19.743 kb on + strand at 19.744 kb on - strand at 19.744 kb on - strand at 19.744 kb on - strand at 20.740 kb on + strand, within H281DRAFT_03520 at 20.743 kb on + strand, within H281DRAFT_03520 at 20.743 kb on + strand, within H281DRAFT_03520 at 20.743 kb on + strand, within H281DRAFT_03520 at 20.743 kb on + strand, within H281DRAFT_03520 at 20.744 kb on - strand, within H281DRAFT_03520 at 20.744 kb on - strand, within H281DRAFT_03520 at 21.209 kb on - strand at 21.226 kb on - strand at 21.428 kb on + strand, within H281DRAFT_03521 at 21.457 kb on - strand at 21.457 kb on - strand at 21.689 kb on + strand at 21.690 kb on - strand at 21.882 kb on - strand, within H281DRAFT_03522 at 21.883 kb on + strand, within H281DRAFT_03522 at 21.921 kb on + strand, within H281DRAFT_03522 at 21.921 kb on + strand, within H281DRAFT_03522 at 21.921 kb on + strand, within H281DRAFT_03522 at 21.922 kb on - strand, within H281DRAFT_03522 at 21.922 kb on - strand, within H281DRAFT_03522 at 21.922 kb on - strand, within H281DRAFT_03522 at 21.929 kb on + strand, within H281DRAFT_03522 at 21.929 kb on + strand, within H281DRAFT_03522 at 21.929 kb on + strand, within H281DRAFT_03522 at 21.929 kb on + strand, within H281DRAFT_03522 at 21.929 kb on + strand, within H281DRAFT_03522 at 21.929 kb on + strand, within H281DRAFT_03522 at 21.930 kb on - strand, within H281DRAFT_03522 at 21.930 kb on - strand, within H281DRAFT_03522 at 22.063 kb on + strand at 22.063 kb on + strand at 22.116 kb on + strand at 22.116 kb on + strand at 22.117 kb on - strand at 22.117 kb on - strand at 22.122 kb on - strand at 22.122 kb on - strand at 22.225 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 18,916 + -0.4 18,916 + +0.1 18,916 + +0.0 18,917 - +0.7 18,917 - +1.9 18,917 - +0.5 18,917 - +0.4 19,090 - +0.8 19,172 - +0.3 19,193 + +0.4 19,224 + -1.0 19,225 - -0.2 19,225 - -0.2 19,225 - -0.9 19,226 + +0.4 19,226 + -0.4 19,226 + +0.2 19,227 - -0.8 19,354 + +0.2 19,355 - +0.0 19,355 - -0.2 19,355 - +0.1 19,355 - -1.0 19,357 + -0.4 19,357 + +0.1 19,432 + H281DRAFT_03519 0.48 +1.2 19,438 + H281DRAFT_03519 0.52 -0.1 19,439 - H281DRAFT_03519 0.52 -1.3 19,531 + +0.3 19,532 - -0.2 19,607 + -0.2 19,607 + +2.9 19,607 + +0.1 19,607 + -0.1 19,608 - -0.1 19,608 - +0.2 19,664 - +1.0 19,664 - +0.7 19,743 + +1.6 19,744 - -1.3 19,744 - -0.1 19,744 - +0.6 20,740 + H281DRAFT_03520 0.64 -0.2 20,743 + H281DRAFT_03520 0.64 -0.1 20,743 + H281DRAFT_03520 0.64 -1.3 20,743 + H281DRAFT_03520 0.64 -0.0 20,743 + H281DRAFT_03520 0.64 -0.8 20,744 - H281DRAFT_03520 0.64 +0.7 20,744 - H281DRAFT_03520 0.64 -0.4 21,209 - +0.9 21,226 - +0.7 21,428 + H281DRAFT_03521 0.32 -0.5 21,457 - +2.9 21,457 - +0.9 21,689 + +0.4 21,690 - +0.4 21,882 - H281DRAFT_03522 0.45 +0.4 21,883 + H281DRAFT_03522 0.46 -0.1 21,921 + H281DRAFT_03522 0.59 -2.1 21,921 + H281DRAFT_03522 0.59 -0.9 21,921 + H281DRAFT_03522 0.59 -0.2 21,922 - H281DRAFT_03522 0.59 -0.9 21,922 - H281DRAFT_03522 0.59 -0.4 21,922 - H281DRAFT_03522 0.59 -0.8 21,929 + H281DRAFT_03522 0.62 -0.6 21,929 + H281DRAFT_03522 0.62 -0.9 21,929 + H281DRAFT_03522 0.62 -2.8 21,929 + H281DRAFT_03522 0.62 -0.6 21,929 + H281DRAFT_03522 0.62 -0.5 21,929 + H281DRAFT_03522 0.62 -0.8 21,930 - H281DRAFT_03522 0.62 +0.1 21,930 - H281DRAFT_03522 0.62 -0.9 22,063 + -0.8 22,063 + -0.3 22,116 + -0.9 22,116 + -0.3 22,117 - +0.0 22,117 - -1.3 22,122 - -0.4 22,122 - -0.3 22,225 + -0.2
Or see this region's nucleotide sequence