Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_03221

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_03220 and H281DRAFT_03221 are separated by 218 nucleotidesH281DRAFT_03221 and H281DRAFT_03222 are separated by 192 nucleotides H281DRAFT_03220: H281DRAFT_03220 - 4-hydroxy-tetrahydrodipicolinate synthase, at 268,155 to 269,087 _03220 H281DRAFT_03221: H281DRAFT_03221 - DNA-binding transcriptional regulator, LysR family, at 269,306 to 270,355 _03221 H281DRAFT_03222: H281DRAFT_03222 - Penicillin binding protein transpeptidase domain-containing protein, at 270,548 to 273,634 _03222 Position (kb) 269 270 271Strain fitness (log2 ratio) -1 0 1at 268.580 kb on - strand, within H281DRAFT_03220at 269.254 kb on + strandat 269.571 kb on + strand, within H281DRAFT_03221at 270.234 kb on + strand, within H281DRAFT_03221at 270.681 kb on + strandat 270.798 kb on - strandat 270.933 kb on - strand, within H281DRAFT_03222at 271.167 kb on + strand, within H281DRAFT_03222at 271.305 kb on - strand, within H281DRAFT_03222at 271.355 kb on + strand, within H281DRAFT_03222

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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268,580 - H281DRAFT_03220 0.46 +1.1
269,254 + +0.5
269,571 + H281DRAFT_03221 0.25 -0.4
270,234 + H281DRAFT_03221 0.88 +0.1
270,681 + +0.4
270,798 - +0.8
270,933 - H281DRAFT_03222 0.12 -0.4
271,167 + H281DRAFT_03222 0.20 -0.3
271,305 - H281DRAFT_03222 0.25 -1.2
271,355 + H281DRAFT_03222 0.26 +0.2

Or see this region's nucleotide sequence