Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02858 and H281DRAFT_02859 are separated by 29 nucleotides H281DRAFT_02859 and H281DRAFT_02860 overlap by 4 nucleotides H281DRAFT_02860 and H281DRAFT_02861 are separated by 5 nucleotides
H281DRAFT_02858: H281DRAFT_02858 - precorrin-4 C11-methyltransferase /precorrin-2 C20-methyltransferase, at 451,966 to 452,691
_02858
H281DRAFT_02859: H281DRAFT_02859 - precorrin-6A reductase (EC 1.3.1.54), at 452,721 to 453,443
_02859
H281DRAFT_02860: H281DRAFT_02860 - cobalt-precorrin-5B (C1)-methyltransferase, at 453,440 to 454,528
_02860
H281DRAFT_02861: H281DRAFT_02861 - precorrin-6Y C5,15-methyltransferase (decarboxylating), at 454,534 to 455,793
_02861
Position (kb)
453
454
455 Strain fitness (log2 ratio)
-1
0
1
2 at 453.275 kb on + strand, within H281DRAFT_02859 at 453.276 kb on - strand, within H281DRAFT_02859 at 453.277 kb on + strand, within H281DRAFT_02859 at 453.278 kb on - strand, within H281DRAFT_02859 at 453.367 kb on + strand, within H281DRAFT_02859 at 453.383 kb on + strand at 453.383 kb on + strand at 453.402 kb on + strand at 453.402 kb on + strand at 453.403 kb on - strand at 453.701 kb on - strand, within H281DRAFT_02860 at 453.787 kb on + strand, within H281DRAFT_02860 at 453.886 kb on + strand, within H281DRAFT_02860 at 453.887 kb on - strand, within H281DRAFT_02860 at 453.904 kb on + strand, within H281DRAFT_02860 at 453.904 kb on + strand, within H281DRAFT_02860 at 453.904 kb on + strand, within H281DRAFT_02860 at 453.904 kb on + strand, within H281DRAFT_02860 at 453.904 kb on + strand, within H281DRAFT_02860 at 453.904 kb on + strand, within H281DRAFT_02860 at 453.905 kb on - strand, within H281DRAFT_02860 at 453.905 kb on - strand, within H281DRAFT_02860 at 453.906 kb on + strand, within H281DRAFT_02860 at 454.098 kb on + strand, within H281DRAFT_02860 at 454.572 kb on + strand at 454.603 kb on + strand at 454.603 kb on + strand at 454.603 kb on + strand at 454.610 kb on + strand at 454.610 kb on + strand at 454.610 kb on + strand at 454.611 kb on - strand at 454.611 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 453,275 + H281DRAFT_02859 0.77 +1.0 453,276 - H281DRAFT_02859 0.77 +1.6 453,277 + H281DRAFT_02859 0.77 +0.9 453,278 - H281DRAFT_02859 0.77 +2.0 453,367 + H281DRAFT_02859 0.89 -0.9 453,383 + -0.0 453,383 + -0.1 453,402 + +1.3 453,402 + -0.3 453,403 - +0.8 453,701 - H281DRAFT_02860 0.24 +1.8 453,787 + H281DRAFT_02860 0.32 +1.0 453,886 + H281DRAFT_02860 0.41 +1.3 453,887 - H281DRAFT_02860 0.41 +1.0 453,904 + H281DRAFT_02860 0.43 -0.3 453,904 + H281DRAFT_02860 0.43 -0.6 453,904 + H281DRAFT_02860 0.43 -0.4 453,904 + H281DRAFT_02860 0.43 +0.4 453,904 + H281DRAFT_02860 0.43 +0.5 453,904 + H281DRAFT_02860 0.43 +1.4 453,905 - H281DRAFT_02860 0.43 +1.9 453,905 - H281DRAFT_02860 0.43 +0.2 453,906 + H281DRAFT_02860 0.43 +0.0 454,098 + H281DRAFT_02860 0.60 -0.3 454,572 + +0.3 454,603 + +1.3 454,603 + -0.3 454,603 + +0.5 454,610 + -1.7 454,610 + -0.2 454,610 + +0.5 454,611 - +0.8 454,611 - +0.2
Or see this region's nucleotide sequence