Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02321 and H281DRAFT_02322 are separated by 243 nucleotides H281DRAFT_02322 and H281DRAFT_02323 are separated by 90 nucleotides H281DRAFT_02323 and H281DRAFT_02324 are separated by 114 nucleotides H281DRAFT_02324 and H281DRAFT_02325 are separated by 150 nucleotides H281DRAFT_02325 and H281DRAFT_02326 are separated by 138 nucleotides
H281DRAFT_02321: H281DRAFT_02321 - Site-specific recombinase XerD, at 477,045 to 478,256
_02321
H281DRAFT_02322: H281DRAFT_02322 - hypothetical protein, at 478,500 to 478,661
_02322
H281DRAFT_02323: H281DRAFT_02323 - hypothetical protein, at 478,752 to 479,096
_02323
H281DRAFT_02324: H281DRAFT_02324 - pyruvate dehydrogenase E1 component, at 479,211 to 481,946
_02324
H281DRAFT_02325: H281DRAFT_02325 - transcriptional regulator, AsnC family, at 482,097 to 482,624
_02325
H281DRAFT_02326: H281DRAFT_02326 - histidine triad (HIT) family protein, at 482,763 to 483,188
_02326
Position (kb) 479
480
481
482 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 478.277 kb on + strand at 478.277 kb on + strand at 478.278 kb on - strand at 478.278 kb on - strand at 478.278 kb on - strand at 478.307 kb on + strand at 478.385 kb on + strand at 478.386 kb on - strand at 478.461 kb on - strand at 478.522 kb on - strand, within H281DRAFT_02322 at 478.522 kb on - strand, within H281DRAFT_02322 at 478.746 kb on + strand at 478.747 kb on - strand at 478.753 kb on + strand at 478.753 kb on + strand at 478.753 kb on + strand at 478.753 kb on + strand at 478.753 kb on + strand at 478.754 kb on - strand at 478.754 kb on - strand at 478.772 kb on + strand at 478.772 kb on + strand at 478.773 kb on - strand at 478.773 kb on - strand at 478.777 kb on + strand at 478.777 kb on + strand at 478.783 kb on + strand at 478.783 kb on + strand at 478.813 kb on + strand, within H281DRAFT_02323 at 478.814 kb on - strand, within H281DRAFT_02323 at 478.834 kb on + strand, within H281DRAFT_02323 at 478.840 kb on + strand, within H281DRAFT_02323 at 478.879 kb on + strand, within H281DRAFT_02323 at 478.879 kb on + strand, within H281DRAFT_02323 at 478.879 kb on + strand, within H281DRAFT_02323 at 478.895 kb on - strand, within H281DRAFT_02323 at 478.909 kb on + strand, within H281DRAFT_02323 at 478.924 kb on + strand, within H281DRAFT_02323 at 478.925 kb on - strand, within H281DRAFT_02323 at 479.002 kb on - strand, within H281DRAFT_02323 at 479.002 kb on - strand, within H281DRAFT_02323 at 479.085 kb on + strand at 479.086 kb on - strand at 479.151 kb on + strand at 479.151 kb on + strand at 479.151 kb on + strand at 479.152 kb on - strand at 479.167 kb on + strand at 479.178 kb on + strand at 479.178 kb on + strand at 479.179 kb on - strand at 479.307 kb on + strand at 479.431 kb on + strand at 479.432 kb on - strand at 479.464 kb on + strand at 479.518 kb on + strand, within H281DRAFT_02324 at 479.518 kb on + strand, within H281DRAFT_02324 at 479.518 kb on + strand, within H281DRAFT_02324 at 479.519 kb on - strand, within H281DRAFT_02324 at 479.675 kb on + strand, within H281DRAFT_02324 at 479.675 kb on + strand, within H281DRAFT_02324 at 479.676 kb on - strand, within H281DRAFT_02324 at 479.741 kb on - strand, within H281DRAFT_02324 at 479.741 kb on - strand, within H281DRAFT_02324 at 479.775 kb on + strand, within H281DRAFT_02324 at 479.775 kb on + strand, within H281DRAFT_02324 at 479.776 kb on - strand, within H281DRAFT_02324 at 479.776 kb on - strand, within H281DRAFT_02324 at 479.807 kb on - strand, within H281DRAFT_02324 at 479.830 kb on + strand, within H281DRAFT_02324 at 479.830 kb on + strand, within H281DRAFT_02324 at 479.831 kb on - strand, within H281DRAFT_02324 at 479.831 kb on - strand, within H281DRAFT_02324 at 479.834 kb on - strand at 479.834 kb on - strand, within H281DRAFT_02324 at 479.834 kb on - strand, within H281DRAFT_02324 at 480.094 kb on + strand, within H281DRAFT_02324 at 480.095 kb on - strand, within H281DRAFT_02324 at 480.095 kb on - strand, within H281DRAFT_02324 at 480.097 kb on + strand at 480.097 kb on + strand, within H281DRAFT_02324 at 480.097 kb on + strand, within H281DRAFT_02324 at 480.097 kb on + strand, within H281DRAFT_02324 at 480.097 kb on + strand, within H281DRAFT_02324 at 480.098 kb on - strand, within H281DRAFT_02324 at 480.098 kb on - strand, within H281DRAFT_02324 at 480.098 kb on - strand, within H281DRAFT_02324 at 480.098 kb on - strand, within H281DRAFT_02324 at 480.098 kb on - strand, within H281DRAFT_02324 at 480.098 kb on - strand, within H281DRAFT_02324 at 480.103 kb on + strand, within H281DRAFT_02324 at 480.103 kb on + strand, within H281DRAFT_02324 at 480.103 kb on + strand, within H281DRAFT_02324 at 480.103 kb on + strand, within H281DRAFT_02324 at 480.103 kb on + strand, within H281DRAFT_02324 at 480.104 kb on - strand, within H281DRAFT_02324 at 480.104 kb on - strand, within H281DRAFT_02324 at 480.104 kb on - strand, within H281DRAFT_02324 at 480.104 kb on - strand at 480.104 kb on - strand, within H281DRAFT_02324 at 480.104 kb on - strand, within H281DRAFT_02324 at 480.104 kb on - strand, within H281DRAFT_02324 at 480.136 kb on + strand at 480.136 kb on + strand, within H281DRAFT_02324 at 480.136 kb on + strand, within H281DRAFT_02324 at 480.137 kb on - strand, within H281DRAFT_02324 at 480.223 kb on + strand, within H281DRAFT_02324 at 480.226 kb on + strand, within H281DRAFT_02324 at 480.226 kb on + strand, within H281DRAFT_02324 at 480.226 kb on + strand, within H281DRAFT_02324 at 480.226 kb on + strand, within H281DRAFT_02324 at 480.227 kb on - strand, within H281DRAFT_02324 at 480.256 kb on + strand, within H281DRAFT_02324 at 480.257 kb on - strand, within H281DRAFT_02324 at 480.277 kb on + strand, within H281DRAFT_02324 at 480.277 kb on + strand, within H281DRAFT_02324 at 480.278 kb on - strand, within H281DRAFT_02324 at 480.278 kb on - strand, within H281DRAFT_02324 at 480.278 kb on - strand, within H281DRAFT_02324 at 480.281 kb on + strand, within H281DRAFT_02324 at 480.281 kb on + strand, within H281DRAFT_02324 at 480.281 kb on + strand, within H281DRAFT_02324 at 480.411 kb on - strand, within H281DRAFT_02324 at 480.411 kb on - strand, within H281DRAFT_02324 at 480.512 kb on - strand, within H281DRAFT_02324 at 480.545 kb on - strand, within H281DRAFT_02324 at 480.577 kb on + strand, within H281DRAFT_02324 at 480.578 kb on - strand, within H281DRAFT_02324 at 480.578 kb on - strand, within H281DRAFT_02324 at 480.578 kb on - strand, within H281DRAFT_02324 at 480.775 kb on + strand, within H281DRAFT_02324 at 480.775 kb on + strand, within H281DRAFT_02324 at 480.898 kb on + strand, within H281DRAFT_02324 at 480.898 kb on + strand, within H281DRAFT_02324 at 480.898 kb on + strand, within H281DRAFT_02324 at 480.944 kb on + strand, within H281DRAFT_02324 at 481.265 kb on - strand, within H281DRAFT_02324 at 481.286 kb on - strand, within H281DRAFT_02324 at 481.399 kb on + strand, within H281DRAFT_02324 at 481.400 kb on - strand, within H281DRAFT_02324 at 481.438 kb on + strand, within H281DRAFT_02324 at 481.450 kb on + strand, within H281DRAFT_02324 at 481.450 kb on + strand, within H281DRAFT_02324 at 481.481 kb on - strand, within H281DRAFT_02324 at 481.573 kb on + strand, within H281DRAFT_02324 at 481.573 kb on + strand, within H281DRAFT_02324 at 481.574 kb on - strand, within H281DRAFT_02324 at 481.574 kb on - strand, within H281DRAFT_02324 at 481.574 kb on - strand, within H281DRAFT_02324 at 481.574 kb on - strand, within H281DRAFT_02324 at 481.604 kb on - strand, within H281DRAFT_02324 at 481.914 kb on + strand at 481.946 kb on + strand at 481.946 kb on + strand at 481.946 kb on + strand at 481.996 kb on + strand at 482.060 kb on - strand at 482.323 kb on + strand, within H281DRAFT_02325 at 482.323 kb on + strand, within H281DRAFT_02325 at 482.324 kb on - strand, within H281DRAFT_02325 at 482.403 kb on + strand, within H281DRAFT_02325 at 482.403 kb on + strand, within H281DRAFT_02325 at 482.404 kb on - strand at 482.427 kb on + strand, within H281DRAFT_02325 at 482.547 kb on + strand at 482.548 kb on - strand, within H281DRAFT_02325 at 482.549 kb on + strand, within H281DRAFT_02325 at 482.549 kb on + strand, within H281DRAFT_02325 at 482.550 kb on - strand, within H281DRAFT_02325 at 482.550 kb on - strand, within H281DRAFT_02325 at 482.550 kb on - strand, within H281DRAFT_02325 at 482.550 kb on - strand, within H281DRAFT_02325 at 482.550 kb on - strand, within H281DRAFT_02325 at 482.581 kb on + strand at 482.677 kb on + strand at 482.677 kb on + strand at 482.678 kb on - strand at 482.678 kb on - strand at 482.678 kb on - strand at 482.678 kb on - strand at 482.680 kb on + strand at 482.680 kb on + strand at 482.681 kb on - strand at 482.697 kb on - strand at 482.769 kb on + strand at 482.813 kb on - strand, within H281DRAFT_02326 at 482.813 kb on - strand, within H281DRAFT_02326
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 478,277 + -0.0 478,277 + -0.0 478,278 - +0.2 478,278 - +0.9 478,278 - -0.5 478,307 + -0.1 478,385 + -0.1 478,386 - +0.8 478,461 - -0.5 478,522 - H281DRAFT_02322 0.14 +0.6 478,522 - H281DRAFT_02322 0.14 +0.5 478,746 + +0.1 478,747 - -1.2 478,753 + +1.2 478,753 + +0.1 478,753 + +0.2 478,753 + -0.5 478,753 + -0.1 478,754 - -0.3 478,754 - +0.2 478,772 + +0.3 478,772 + +1.4 478,773 - +1.9 478,773 - +0.7 478,777 + +0.4 478,777 + -0.5 478,783 + -1.3 478,783 + -0.6 478,813 + H281DRAFT_02323 0.18 -0.1 478,814 - H281DRAFT_02323 0.18 +0.2 478,834 + H281DRAFT_02323 0.24 -1.6 478,840 + H281DRAFT_02323 0.26 +0.1 478,879 + H281DRAFT_02323 0.37 +0.5 478,879 + H281DRAFT_02323 0.37 +0.6 478,879 + H281DRAFT_02323 0.37 -0.1 478,895 - H281DRAFT_02323 0.41 +0.0 478,909 + H281DRAFT_02323 0.46 +0.1 478,924 + H281DRAFT_02323 0.50 -0.3 478,925 - H281DRAFT_02323 0.50 -0.1 479,002 - H281DRAFT_02323 0.72 -1.7 479,002 - H281DRAFT_02323 0.72 -0.2 479,085 + -0.1 479,086 - +2.7 479,151 + +0.1 479,151 + +0.6 479,151 + -1.0 479,152 - +0.8 479,167 + +0.2 479,178 + -0.4 479,178 + -0.1 479,179 - -1.3 479,307 + +0.2 479,431 + +0.2 479,432 - +0.5 479,464 + +1.0 479,518 + H281DRAFT_02324 0.11 -0.7 479,518 + H281DRAFT_02324 0.11 +0.8 479,518 + H281DRAFT_02324 0.11 +0.9 479,519 - H281DRAFT_02324 0.11 +0.5 479,675 + H281DRAFT_02324 0.17 -0.3 479,675 + H281DRAFT_02324 0.17 +0.1 479,676 - H281DRAFT_02324 0.17 -0.1 479,741 - H281DRAFT_02324 0.19 +0.2 479,741 - H281DRAFT_02324 0.19 +0.3 479,775 + H281DRAFT_02324 0.21 +0.2 479,775 + H281DRAFT_02324 0.21 +0.1 479,776 - H281DRAFT_02324 0.21 +0.4 479,776 - H281DRAFT_02324 0.21 +0.1 479,807 - H281DRAFT_02324 0.22 +1.1 479,830 + H281DRAFT_02324 0.23 -0.9 479,830 + H281DRAFT_02324 0.23 +0.2 479,831 - H281DRAFT_02324 0.23 +0.8 479,831 - H281DRAFT_02324 0.23 -0.1 479,834 - +1.7 479,834 - H281DRAFT_02324 0.23 -0.5 479,834 - H281DRAFT_02324 0.23 -0.2 480,094 + H281DRAFT_02324 0.32 -0.3 480,095 - H281DRAFT_02324 0.32 +0.5 480,095 - H281DRAFT_02324 0.32 +0.1 480,097 + +2.1 480,097 + H281DRAFT_02324 0.32 +0.6 480,097 + H281DRAFT_02324 0.32 -0.5 480,097 + H281DRAFT_02324 0.32 +0.3 480,097 + H281DRAFT_02324 0.32 -1.8 480,098 - H281DRAFT_02324 0.32 -0.0 480,098 - H281DRAFT_02324 0.32 -0.2 480,098 - H281DRAFT_02324 0.32 -0.2 480,098 - H281DRAFT_02324 0.32 +0.2 480,098 - H281DRAFT_02324 0.32 +0.6 480,098 - H281DRAFT_02324 0.32 -1.9 480,103 + H281DRAFT_02324 0.33 +0.1 480,103 + H281DRAFT_02324 0.33 -0.1 480,103 + H281DRAFT_02324 0.33 +0.3 480,103 + H281DRAFT_02324 0.33 -0.1 480,103 + H281DRAFT_02324 0.33 -0.1 480,104 - H281DRAFT_02324 0.33 -1.0 480,104 - H281DRAFT_02324 0.33 -1.3 480,104 - H281DRAFT_02324 0.33 +0.6 480,104 - +0.1 480,104 - H281DRAFT_02324 0.33 -0.7 480,104 - H281DRAFT_02324 0.33 +1.3 480,104 - H281DRAFT_02324 0.33 -0.5 480,136 + +3.4 480,136 + H281DRAFT_02324 0.34 -0.0 480,136 + H281DRAFT_02324 0.34 -0.5 480,137 - H281DRAFT_02324 0.34 -0.5 480,223 + H281DRAFT_02324 0.37 +0.2 480,226 + H281DRAFT_02324 0.37 +0.7 480,226 + H281DRAFT_02324 0.37 +0.0 480,226 + H281DRAFT_02324 0.37 +1.2 480,226 + H281DRAFT_02324 0.37 -0.7 480,227 - H281DRAFT_02324 0.37 -0.1 480,256 + H281DRAFT_02324 0.38 +2.1 480,257 - H281DRAFT_02324 0.38 -0.1 480,277 + H281DRAFT_02324 0.39 +0.7 480,277 + H281DRAFT_02324 0.39 +1.4 480,278 - H281DRAFT_02324 0.39 +0.4 480,278 - H281DRAFT_02324 0.39 -0.4 480,278 - H281DRAFT_02324 0.39 -0.7 480,281 + H281DRAFT_02324 0.39 -0.1 480,281 + H281DRAFT_02324 0.39 +0.0 480,281 + H281DRAFT_02324 0.39 -1.7 480,411 - H281DRAFT_02324 0.44 +0.4 480,411 - H281DRAFT_02324 0.44 -0.7 480,512 - H281DRAFT_02324 0.48 -1.0 480,545 - H281DRAFT_02324 0.49 -0.7 480,577 + H281DRAFT_02324 0.50 +0.1 480,578 - H281DRAFT_02324 0.50 -0.3 480,578 - H281DRAFT_02324 0.50 -0.0 480,578 - H281DRAFT_02324 0.50 +0.3 480,775 + H281DRAFT_02324 0.57 +0.8 480,775 + H281DRAFT_02324 0.57 +0.6 480,898 + H281DRAFT_02324 0.62 -1.2 480,898 + H281DRAFT_02324 0.62 +0.4 480,898 + H281DRAFT_02324 0.62 +0.2 480,944 + H281DRAFT_02324 0.63 +0.6 481,265 - H281DRAFT_02324 0.75 -0.5 481,286 - H281DRAFT_02324 0.76 -0.9 481,399 + H281DRAFT_02324 0.80 +0.4 481,400 - H281DRAFT_02324 0.80 -0.3 481,438 + H281DRAFT_02324 0.81 +0.6 481,450 + H281DRAFT_02324 0.82 +0.4 481,450 + H281DRAFT_02324 0.82 +1.0 481,481 - H281DRAFT_02324 0.83 +0.6 481,573 + H281DRAFT_02324 0.86 +0.5 481,573 + H281DRAFT_02324 0.86 -1.6 481,574 - H281DRAFT_02324 0.86 -0.1 481,574 - H281DRAFT_02324 0.86 -0.9 481,574 - H281DRAFT_02324 0.86 +0.4 481,574 - H281DRAFT_02324 0.86 -1.7 481,604 - H281DRAFT_02324 0.87 -0.0 481,914 + +0.7 481,946 + -0.3 481,946 + +0.5 481,946 + -0.5 481,996 + +1.1 482,060 - -0.3 482,323 + H281DRAFT_02325 0.43 +0.8 482,323 + H281DRAFT_02325 0.43 -0.7 482,324 - H281DRAFT_02325 0.43 +0.0 482,403 + H281DRAFT_02325 0.58 -0.9 482,403 + H281DRAFT_02325 0.58 +0.6 482,404 - +2.1 482,427 + H281DRAFT_02325 0.62 +0.1 482,547 + -1.2 482,548 - H281DRAFT_02325 0.85 -1.8 482,549 + H281DRAFT_02325 0.86 +1.2 482,549 + H281DRAFT_02325 0.86 -0.6 482,550 - H281DRAFT_02325 0.86 +0.1 482,550 - H281DRAFT_02325 0.86 +0.9 482,550 - H281DRAFT_02325 0.86 -0.2 482,550 - H281DRAFT_02325 0.86 +0.7 482,550 - H281DRAFT_02325 0.86 -1.5 482,581 + +0.6 482,677 + +0.5 482,677 + -1.0 482,678 - +0.4 482,678 - -0.9 482,678 - +0.3 482,678 - +1.0 482,680 + +1.0 482,680 + +1.2 482,681 - -1.4 482,697 - -0.0 482,769 + -0.7 482,813 - H281DRAFT_02326 0.12 +0.5 482,813 - H281DRAFT_02326 0.12 +0.7
Or see this region's nucleotide sequence