Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_02101

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_02100 and H281DRAFT_02101 are separated by 76 nucleotidesH281DRAFT_02101 and H281DRAFT_02102 are separated by 61 nucleotidesH281DRAFT_02102 and H281DRAFT_02103 are separated by 4 nucleotidesH281DRAFT_02103 and H281DRAFT_02104 overlap by 4 nucleotides H281DRAFT_02100: H281DRAFT_02100 - glyoxylate/hydroxypyruvate reductase A, at 226,953 to 227,894 _02100 H281DRAFT_02101: H281DRAFT_02101 - hydroxymethylglutaryl-CoA lyase, at 227,971 to 228,897 _02101 H281DRAFT_02102: H281DRAFT_02102 - Cys-tRNA(Pro) deacylase, prolyl-tRNA editing enzyme YbaK/EbsC, at 228,959 to 229,480 _02102 H281DRAFT_02103: H281DRAFT_02103 - hypothetical protein, at 229,485 to 229,712 _02103 H281DRAFT_02104: H281DRAFT_02104 - Acyl dehydratase, at 229,709 to 230,194 _02104 Position (kb) 227 228 229Strain fitness (log2 ratio) -2 -1 0 1 2at 227.083 kb on + strand, within H281DRAFT_02100at 227.084 kb on - strand, within H281DRAFT_02100at 227.260 kb on - strand, within H281DRAFT_02100at 227.264 kb on - strand, within H281DRAFT_02100at 227.264 kb on - strand, within H281DRAFT_02100at 227.264 kb on - strand, within H281DRAFT_02100at 227.283 kb on + strand, within H281DRAFT_02100at 227.284 kb on - strand, within H281DRAFT_02100at 227.304 kb on + strand, within H281DRAFT_02100at 227.339 kb on - strand, within H281DRAFT_02100at 227.339 kb on - strand, within H281DRAFT_02100at 227.449 kb on - strand, within H281DRAFT_02100at 227.450 kb on + strand, within H281DRAFT_02100at 227.451 kb on - strand, within H281DRAFT_02100at 227.451 kb on - strand, within H281DRAFT_02100at 227.483 kb on + strand, within H281DRAFT_02100at 227.484 kb on - strand, within H281DRAFT_02100at 227.616 kb on + strand, within H281DRAFT_02100at 227.616 kb on + strand, within H281DRAFT_02100at 227.617 kb on - strand, within H281DRAFT_02100at 227.617 kb on - strand, within H281DRAFT_02100at 227.771 kb on + strand, within H281DRAFT_02100at 227.772 kb on - strand, within H281DRAFT_02100at 227.949 kb on + strandat 227.949 kb on + strandat 227.950 kb on - strandat 227.956 kb on + strandat 227.956 kb on + strandat 227.957 kb on - strandat 227.957 kb on - strandat 227.957 kb on - strandat 228.200 kb on - strandat 228.419 kb on - strand, within H281DRAFT_02101at 228.419 kb on - strand, within H281DRAFT_02101at 228.504 kb on + strand, within H281DRAFT_02101at 228.604 kb on + strand, within H281DRAFT_02101at 228.628 kb on + strand, within H281DRAFT_02101at 228.659 kb on - strand, within H281DRAFT_02101at 228.695 kb on - strand, within H281DRAFT_02101at 228.739 kb on + strand, within H281DRAFT_02101at 228.739 kb on + strand, within H281DRAFT_02101at 228.901 kb on + strandat 228.902 kb on - strandat 228.942 kb on + strandat 229.225 kb on - strand, within H281DRAFT_02102at 229.287 kb on - strand, within H281DRAFT_02102at 229.287 kb on - strand, within H281DRAFT_02102at 229.624 kb on - strand, within H281DRAFT_02103at 229.838 kb on + strand, within H281DRAFT_02104at 229.839 kb on - strand, within H281DRAFT_02104

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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227,083 + H281DRAFT_02100 0.14 -0.5
227,084 - H281DRAFT_02100 0.14 +0.5
227,260 - H281DRAFT_02100 0.33 -0.9
227,264 - H281DRAFT_02100 0.33 +0.8
227,264 - H281DRAFT_02100 0.33 +0.4
227,264 - H281DRAFT_02100 0.33 -1.8
227,283 + H281DRAFT_02100 0.35 -0.2
227,284 - H281DRAFT_02100 0.35 -0.5
227,304 + H281DRAFT_02100 0.37 -0.2
227,339 - H281DRAFT_02100 0.41 +0.5
227,339 - H281DRAFT_02100 0.41 +0.6
227,449 - H281DRAFT_02100 0.53 +0.3
227,450 + H281DRAFT_02100 0.53 -0.2
227,451 - H281DRAFT_02100 0.53 +0.4
227,451 - H281DRAFT_02100 0.53 +0.1
227,483 + H281DRAFT_02100 0.56 -1.3
227,484 - H281DRAFT_02100 0.56 +0.2
227,616 + H281DRAFT_02100 0.70 +0.7
227,616 + H281DRAFT_02100 0.70 +0.0
227,617 - H281DRAFT_02100 0.70 -0.3
227,617 - H281DRAFT_02100 0.70 +0.4
227,771 + H281DRAFT_02100 0.87 -0.0
227,772 - H281DRAFT_02100 0.87 -0.2
227,949 + -1.7
227,949 + +0.2
227,950 - +0.4
227,956 + -1.3
227,956 + -0.4
227,957 - +0.7
227,957 - +0.9
227,957 - -0.5
228,200 - +1.1
228,419 - H281DRAFT_02101 0.48 -2.2
228,419 - H281DRAFT_02101 0.48 +0.3
228,504 + H281DRAFT_02101 0.57 -0.1
228,604 + H281DRAFT_02101 0.68 +2.2
228,628 + H281DRAFT_02101 0.71 -0.7
228,659 - H281DRAFT_02101 0.74 -0.6
228,695 - H281DRAFT_02101 0.78 -0.1
228,739 + H281DRAFT_02101 0.83 -1.5
228,739 + H281DRAFT_02101 0.83 -0.5
228,901 + +0.2
228,902 - -0.6
228,942 + -1.0
229,225 - H281DRAFT_02102 0.51 +1.3
229,287 - H281DRAFT_02102 0.63 -0.5
229,287 - H281DRAFT_02102 0.63 +0.9
229,624 - H281DRAFT_02103 0.61 -2.4
229,838 + H281DRAFT_02104 0.27 +0.5
229,839 - H281DRAFT_02104 0.27 -0.1

Or see this region's nucleotide sequence