Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02100 and H281DRAFT_02101 are separated by 76 nucleotides H281DRAFT_02101 and H281DRAFT_02102 are separated by 61 nucleotides H281DRAFT_02102 and H281DRAFT_02103 are separated by 4 nucleotides H281DRAFT_02103 and H281DRAFT_02104 overlap by 4 nucleotides
H281DRAFT_02100: H281DRAFT_02100 - glyoxylate/hydroxypyruvate reductase A, at 226,953 to 227,894
_02100
H281DRAFT_02101: H281DRAFT_02101 - hydroxymethylglutaryl-CoA lyase, at 227,971 to 228,897
_02101
H281DRAFT_02102: H281DRAFT_02102 - Cys-tRNA(Pro) deacylase, prolyl-tRNA editing enzyme YbaK/EbsC, at 228,959 to 229,480
_02102
H281DRAFT_02103: H281DRAFT_02103 - hypothetical protein, at 229,485 to 229,712
_02103
H281DRAFT_02104: H281DRAFT_02104 - Acyl dehydratase, at 229,709 to 230,194
_02104
Position (kb)
227
228
229 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 227.083 kb on + strand, within H281DRAFT_02100 at 227.084 kb on - strand, within H281DRAFT_02100 at 227.260 kb on - strand, within H281DRAFT_02100 at 227.264 kb on - strand, within H281DRAFT_02100 at 227.264 kb on - strand, within H281DRAFT_02100 at 227.264 kb on - strand, within H281DRAFT_02100 at 227.283 kb on + strand, within H281DRAFT_02100 at 227.284 kb on - strand, within H281DRAFT_02100 at 227.304 kb on + strand, within H281DRAFT_02100 at 227.339 kb on - strand, within H281DRAFT_02100 at 227.339 kb on - strand, within H281DRAFT_02100 at 227.449 kb on - strand, within H281DRAFT_02100 at 227.450 kb on + strand, within H281DRAFT_02100 at 227.451 kb on - strand, within H281DRAFT_02100 at 227.451 kb on - strand, within H281DRAFT_02100 at 227.483 kb on + strand, within H281DRAFT_02100 at 227.484 kb on - strand, within H281DRAFT_02100 at 227.616 kb on + strand, within H281DRAFT_02100 at 227.616 kb on + strand, within H281DRAFT_02100 at 227.617 kb on - strand, within H281DRAFT_02100 at 227.617 kb on - strand, within H281DRAFT_02100 at 227.771 kb on + strand, within H281DRAFT_02100 at 227.772 kb on - strand, within H281DRAFT_02100 at 227.949 kb on + strand at 227.949 kb on + strand at 227.950 kb on - strand at 227.956 kb on + strand at 227.956 kb on + strand at 227.957 kb on - strand at 227.957 kb on - strand at 227.957 kb on - strand at 228.200 kb on - strand at 228.419 kb on - strand, within H281DRAFT_02101 at 228.419 kb on - strand, within H281DRAFT_02101 at 228.504 kb on + strand, within H281DRAFT_02101 at 228.604 kb on + strand, within H281DRAFT_02101 at 228.628 kb on + strand, within H281DRAFT_02101 at 228.659 kb on - strand, within H281DRAFT_02101 at 228.695 kb on - strand, within H281DRAFT_02101 at 228.739 kb on + strand, within H281DRAFT_02101 at 228.739 kb on + strand, within H281DRAFT_02101 at 228.901 kb on + strand at 228.902 kb on - strand at 228.942 kb on + strand at 229.225 kb on - strand, within H281DRAFT_02102 at 229.287 kb on - strand, within H281DRAFT_02102 at 229.287 kb on - strand, within H281DRAFT_02102 at 229.624 kb on - strand, within H281DRAFT_02103 at 229.838 kb on + strand, within H281DRAFT_02104 at 229.839 kb on - strand, within H281DRAFT_02104
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 227,083 + H281DRAFT_02100 0.14 -0.5 227,084 - H281DRAFT_02100 0.14 +0.5 227,260 - H281DRAFT_02100 0.33 -0.9 227,264 - H281DRAFT_02100 0.33 +0.8 227,264 - H281DRAFT_02100 0.33 +0.4 227,264 - H281DRAFT_02100 0.33 -1.8 227,283 + H281DRAFT_02100 0.35 -0.2 227,284 - H281DRAFT_02100 0.35 -0.5 227,304 + H281DRAFT_02100 0.37 -0.2 227,339 - H281DRAFT_02100 0.41 +0.5 227,339 - H281DRAFT_02100 0.41 +0.6 227,449 - H281DRAFT_02100 0.53 +0.3 227,450 + H281DRAFT_02100 0.53 -0.2 227,451 - H281DRAFT_02100 0.53 +0.4 227,451 - H281DRAFT_02100 0.53 +0.1 227,483 + H281DRAFT_02100 0.56 -1.3 227,484 - H281DRAFT_02100 0.56 +0.2 227,616 + H281DRAFT_02100 0.70 +0.7 227,616 + H281DRAFT_02100 0.70 +0.0 227,617 - H281DRAFT_02100 0.70 -0.3 227,617 - H281DRAFT_02100 0.70 +0.4 227,771 + H281DRAFT_02100 0.87 -0.0 227,772 - H281DRAFT_02100 0.87 -0.2 227,949 + -1.7 227,949 + +0.2 227,950 - +0.4 227,956 + -1.3 227,956 + -0.4 227,957 - +0.7 227,957 - +0.9 227,957 - -0.5 228,200 - +1.1 228,419 - H281DRAFT_02101 0.48 -2.2 228,419 - H281DRAFT_02101 0.48 +0.3 228,504 + H281DRAFT_02101 0.57 -0.1 228,604 + H281DRAFT_02101 0.68 +2.2 228,628 + H281DRAFT_02101 0.71 -0.7 228,659 - H281DRAFT_02101 0.74 -0.6 228,695 - H281DRAFT_02101 0.78 -0.1 228,739 + H281DRAFT_02101 0.83 -1.5 228,739 + H281DRAFT_02101 0.83 -0.5 228,901 + +0.2 228,902 - -0.6 228,942 + -1.0 229,225 - H281DRAFT_02102 0.51 +1.3 229,287 - H281DRAFT_02102 0.63 -0.5 229,287 - H281DRAFT_02102 0.63 +0.9 229,624 - H281DRAFT_02103 0.61 -2.4 229,838 + H281DRAFT_02104 0.27 +0.5 229,839 - H281DRAFT_02104 0.27 -0.1
Or see this region's nucleotide sequence