Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02087 and H281DRAFT_02088 are separated by 94 nucleotides H281DRAFT_02088 and H281DRAFT_02089 overlap by 8 nucleotides H281DRAFT_02089 and H281DRAFT_02090 are separated by 46 nucleotides H281DRAFT_02090 and H281DRAFT_02091 are separated by 12 nucleotides
H281DRAFT_02087: H281DRAFT_02087 - isocitrate dehydrogenase kinase/phosphatase, at 211,469 to 213,316
_02087
H281DRAFT_02088: H281DRAFT_02088 - carbonic anhydrase, at 213,411 to 214,187
_02088
H281DRAFT_02089: H281DRAFT_02089 - acetyl-CoA C-acetyltransferase, at 214,180 to 215,388
_02089
H281DRAFT_02090: H281DRAFT_02090 - Short-chain dehydrogenase, at 215,435 to 216,112
_02090
H281DRAFT_02091: H281DRAFT_02091 - hypothetical protein, at 216,125 to 217,261
_02091
Position (kb) 214
215
216 Strain fitness (log2 ratio) -2
-1
0
1 at 213.346 kb on + strand at 213.347 kb on - strand at 213.360 kb on + strand at 213.365 kb on + strand at 213.366 kb on - strand at 213.393 kb on + strand at 213.410 kb on + strand at 213.543 kb on + strand, within H281DRAFT_02088 at 213.543 kb on + strand, within H281DRAFT_02088 at 213.624 kb on - strand, within H281DRAFT_02088 at 213.640 kb on + strand, within H281DRAFT_02088 at 213.650 kb on + strand, within H281DRAFT_02088 at 213.651 kb on - strand, within H281DRAFT_02088 at 213.730 kb on - strand, within H281DRAFT_02088 at 213.979 kb on - strand, within H281DRAFT_02088 at 214.174 kb on + strand at 214.174 kb on + strand at 214.174 kb on + strand at 214.223 kb on + strand at 214.223 kb on + strand at 214.224 kb on - strand at 214.361 kb on + strand, within H281DRAFT_02089 at 214.362 kb on - strand, within H281DRAFT_02089 at 214.362 kb on - strand, within H281DRAFT_02089 at 214.362 kb on - strand, within H281DRAFT_02089 at 214.576 kb on + strand, within H281DRAFT_02089 at 214.577 kb on - strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.635 kb on + strand, within H281DRAFT_02089 at 214.636 kb on - strand, within H281DRAFT_02089 at 214.636 kb on - strand, within H281DRAFT_02089 at 214.636 kb on - strand, within H281DRAFT_02089 at 214.636 kb on - strand, within H281DRAFT_02089 at 214.636 kb on - strand, within H281DRAFT_02089 at 214.636 kb on - strand, within H281DRAFT_02089 at 215.277 kb on + strand at 215.394 kb on - strand at 215.397 kb on - strand at 215.397 kb on - strand at 215.503 kb on + strand, within H281DRAFT_02090 at 215.504 kb on - strand, within H281DRAFT_02090 at 215.579 kb on + strand, within H281DRAFT_02090 at 215.579 kb on + strand, within H281DRAFT_02090 at 215.675 kb on + strand, within H281DRAFT_02090 at 215.870 kb on + strand, within H281DRAFT_02090 at 215.871 kb on - strand, within H281DRAFT_02090 at 216.087 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 213,346 + +0.6 213,347 - +1.2 213,360 + -0.4 213,365 + -0.3 213,366 - -0.1 213,393 + -2.4 213,410 + -0.4 213,543 + H281DRAFT_02088 0.17 +0.3 213,543 + H281DRAFT_02088 0.17 +0.5 213,624 - H281DRAFT_02088 0.27 -0.5 213,640 + H281DRAFT_02088 0.29 +1.3 213,650 + H281DRAFT_02088 0.31 +0.5 213,651 - H281DRAFT_02088 0.31 +1.4 213,730 - H281DRAFT_02088 0.41 -0.3 213,979 - H281DRAFT_02088 0.73 +0.6 214,174 + -0.2 214,174 + +0.6 214,174 + +0.3 214,223 + +0.6 214,223 + -0.0 214,224 - +0.2 214,361 + H281DRAFT_02089 0.15 +0.3 214,362 - H281DRAFT_02089 0.15 +0.3 214,362 - H281DRAFT_02089 0.15 +1.2 214,362 - H281DRAFT_02089 0.15 +0.9 214,576 + H281DRAFT_02089 0.33 -0.3 214,577 - H281DRAFT_02089 0.33 +0.5 214,635 + H281DRAFT_02089 0.38 -0.4 214,635 + H281DRAFT_02089 0.38 -0.5 214,635 + H281DRAFT_02089 0.38 +0.4 214,635 + H281DRAFT_02089 0.38 -0.4 214,635 + H281DRAFT_02089 0.38 +1.1 214,635 + H281DRAFT_02089 0.38 -1.0 214,635 + H281DRAFT_02089 0.38 -2.4 214,636 - H281DRAFT_02089 0.38 +1.2 214,636 - H281DRAFT_02089 0.38 +0.4 214,636 - H281DRAFT_02089 0.38 +1.1 214,636 - H281DRAFT_02089 0.38 +0.1 214,636 - H281DRAFT_02089 0.38 -0.9 214,636 - H281DRAFT_02089 0.38 +1.1 215,277 + -1.1 215,394 - +0.4 215,397 - -0.6 215,397 - +0.3 215,503 + H281DRAFT_02090 0.10 -0.2 215,504 - H281DRAFT_02090 0.10 -0.5 215,579 + H281DRAFT_02090 0.21 -0.7 215,579 + H281DRAFT_02090 0.21 -0.1 215,675 + H281DRAFT_02090 0.35 +0.6 215,870 + H281DRAFT_02090 0.64 +0.0 215,871 - H281DRAFT_02090 0.64 +0.5 216,087 - +0.5
Or see this region's nucleotide sequence