Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_02012

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_02011 and H281DRAFT_02012 are separated by 247 nucleotidesH281DRAFT_02012 and H281DRAFT_02013 are separated by 45 nucleotidesH281DRAFT_02013 and H281DRAFT_02014 are separated by 466 nucleotides H281DRAFT_02011: H281DRAFT_02011 - glutamate--cysteine ligase, at 128,218 to 129,507 _02011 H281DRAFT_02012: H281DRAFT_02012 - ammonium transporter, at 129,755 to 131,248 _02012 H281DRAFT_02013: H281DRAFT_02013 - nitrogen regulatory protein P-II family, at 131,294 to 131,632 _02013 H281DRAFT_02014: H281DRAFT_02014 - hypothetical protein, at 132,099 to 132,353 _02014 Position (kb) 129 130 131 132Strain fitness (log2 ratio) -2 -1 0 1 2 3at 128.805 kb on - strandat 128.868 kb on - strand, within H281DRAFT_02011at 128.930 kb on + strand, within H281DRAFT_02011at 128.931 kb on - strand, within H281DRAFT_02011at 128.931 kb on - strand, within H281DRAFT_02011at 129.380 kb on + strandat 129.380 kb on + strandat 129.380 kb on + strandat 129.380 kb on + strandat 129.381 kb on - strandat 129.381 kb on - strandat 129.482 kb on - strandat 129.489 kb on + strandat 129.493 kb on + strandat 129.493 kb on + strandat 129.493 kb on + strandat 129.494 kb on - strandat 129.494 kb on - strandat 129.539 kb on + strandat 129.539 kb on + strandat 129.539 kb on + strandat 129.618 kb on + strandat 129.646 kb on + strandat 129.757 kb on - strandat 129.876 kb on + strandat 129.876 kb on + strandat 129.876 kb on + strandat 129.876 kb on + strandat 129.876 kb on + strandat 129.876 kb on + strandat 130.183 kb on + strand, within H281DRAFT_02012at 130.238 kb on + strand, within H281DRAFT_02012at 130.238 kb on + strand, within H281DRAFT_02012at 130.239 kb on - strand, within H281DRAFT_02012at 130.530 kb on + strand, within H281DRAFT_02012at 130.582 kb on + strand, within H281DRAFT_02012at 130.582 kb on + strand, within H281DRAFT_02012at 130.582 kb on + strand, within H281DRAFT_02012at 130.583 kb on - strand, within H281DRAFT_02012at 130.583 kb on - strand, within H281DRAFT_02012at 130.583 kb on - strand, within H281DRAFT_02012at 130.641 kb on + strand, within H281DRAFT_02012at 130.642 kb on - strand, within H281DRAFT_02012at 130.650 kb on + strand, within H281DRAFT_02012at 130.650 kb on + strand, within H281DRAFT_02012at 130.651 kb on - strand, within H281DRAFT_02012at 130.719 kb on + strand, within H281DRAFT_02012at 130.719 kb on + strand, within H281DRAFT_02012at 130.719 kb on + strand, within H281DRAFT_02012at 130.719 kb on + strand, within H281DRAFT_02012at 130.719 kb on + strand, within H281DRAFT_02012at 130.719 kb on + strand, within H281DRAFT_02012at 130.719 kb on + strand, within H281DRAFT_02012at 130.720 kb on - strand, within H281DRAFT_02012at 130.720 kb on - strand, within H281DRAFT_02012at 130.720 kb on - strand, within H281DRAFT_02012at 130.720 kb on - strand, within H281DRAFT_02012at 130.791 kb on + strand, within H281DRAFT_02012at 130.792 kb on - strand, within H281DRAFT_02012at 130.792 kb on - strand, within H281DRAFT_02012at 130.859 kb on + strand, within H281DRAFT_02012at 130.859 kb on + strand, within H281DRAFT_02012at 130.859 kb on + strand, within H281DRAFT_02012at 130.860 kb on - strand, within H281DRAFT_02012at 130.888 kb on - strand, within H281DRAFT_02012at 131.141 kb on - strandat 131.237 kb on + strandat 131.237 kb on + strandat 131.237 kb on + strandat 131.238 kb on - strandat 131.238 kb on - strandat 131.270 kb on + strandat 131.271 kb on - strandat 131.271 kb on - strandat 131.271 kb on - strandat 131.282 kb on + strandat 131.295 kb on + strandat 131.295 kb on + strandat 131.296 kb on - strandat 131.296 kb on - strandat 131.296 kb on - strandat 131.481 kb on + strand, within H281DRAFT_02013at 131.481 kb on + strand, within H281DRAFT_02013at 131.481 kb on + strand, within H281DRAFT_02013at 131.496 kb on + strand, within H281DRAFT_02013at 131.506 kb on + strand, within H281DRAFT_02013at 131.506 kb on + strand, within H281DRAFT_02013at 131.506 kb on + strand, within H281DRAFT_02013at 131.646 kb on + strandat 131.646 kb on + strandat 131.646 kb on + strandat 131.646 kb on + strandat 131.647 kb on - strandat 131.647 kb on - strandat 131.647 kb on - strandat 131.647 kb on - strandat 131.647 kb on - strandat 131.647 kb on - strandat 131.679 kb on + strandat 131.692 kb on + strandat 131.732 kb on - strandat 131.756 kb on + strandat 131.757 kb on - strandat 131.819 kb on - strandat 131.901 kb on + strandat 131.943 kb on + strandat 131.943 kb on + strandat 131.943 kb on + strandat 131.943 kb on + strandat 131.943 kb on + strandat 131.944 kb on - strandat 131.944 kb on - strandat 131.944 kb on - strandat 132.057 kb on + strandat 132.057 kb on + strandat 132.058 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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128,805 - +3.4
128,868 - H281DRAFT_02011 0.50 -0.1
128,930 + H281DRAFT_02011 0.55 +0.5
128,931 - H281DRAFT_02011 0.55 +0.0
128,931 - H281DRAFT_02011 0.55 -1.0
129,380 + -1.5
129,380 + -0.5
129,380 + -0.4
129,380 + +0.5
129,381 - -0.3
129,381 - +0.2
129,482 - +0.3
129,489 + +0.5
129,493 + +0.1
129,493 + +0.8
129,493 + -0.3
129,494 - -0.5
129,494 - +0.2
129,539 + +0.2
129,539 + +1.1
129,539 + +0.3
129,618 + +0.6
129,646 + +0.7
129,757 - +0.0
129,876 + -0.3
129,876 + -0.1
129,876 + -0.0
129,876 + +2.9
129,876 + -1.2
129,876 + +0.6
130,183 + H281DRAFT_02012 0.29 +1.3
130,238 + H281DRAFT_02012 0.32 -0.4
130,238 + H281DRAFT_02012 0.32 +1.3
130,239 - H281DRAFT_02012 0.32 +1.1
130,530 + H281DRAFT_02012 0.52 +0.1
130,582 + H281DRAFT_02012 0.55 +1.0
130,582 + H281DRAFT_02012 0.55 -1.2
130,582 + H281DRAFT_02012 0.55 +0.5
130,583 - H281DRAFT_02012 0.55 +1.0
130,583 - H281DRAFT_02012 0.55 +0.6
130,583 - H281DRAFT_02012 0.55 -0.3
130,641 + H281DRAFT_02012 0.59 +0.5
130,642 - H281DRAFT_02012 0.59 -1.3
130,650 + H281DRAFT_02012 0.60 -0.5
130,650 + H281DRAFT_02012 0.60 -0.0
130,651 - H281DRAFT_02012 0.60 -0.4
130,719 + H281DRAFT_02012 0.65 +0.3
130,719 + H281DRAFT_02012 0.65 +0.9
130,719 + H281DRAFT_02012 0.65 -1.1
130,719 + H281DRAFT_02012 0.65 +2.6
130,719 + H281DRAFT_02012 0.65 -0.5
130,719 + H281DRAFT_02012 0.65 +1.0
130,719 + H281DRAFT_02012 0.65 -0.0
130,720 - H281DRAFT_02012 0.65 +0.0
130,720 - H281DRAFT_02012 0.65 -0.3
130,720 - H281DRAFT_02012 0.65 -1.2
130,720 - H281DRAFT_02012 0.65 -0.4
130,791 + H281DRAFT_02012 0.69 +1.0
130,792 - H281DRAFT_02012 0.69 -0.6
130,792 - H281DRAFT_02012 0.69 +0.1
130,859 + H281DRAFT_02012 0.74 -1.0
130,859 + H281DRAFT_02012 0.74 +0.5
130,859 + H281DRAFT_02012 0.74 +0.5
130,860 - H281DRAFT_02012 0.74 +0.5
130,888 - H281DRAFT_02012 0.76 +0.0
131,141 - -0.1
131,237 + -0.9
131,237 + +0.5
131,237 + -0.4
131,238 - -0.6
131,238 - +0.7
131,270 + -0.4
131,271 - -0.3
131,271 - +0.2
131,271 - -2.5
131,282 + +0.8
131,295 + +0.7
131,295 + +1.8
131,296 - -2.2
131,296 - -1.4
131,296 - -1.9
131,481 + H281DRAFT_02013 0.55 -0.3
131,481 + H281DRAFT_02013 0.55 +0.1
131,481 + H281DRAFT_02013 0.55 +0.2
131,496 + H281DRAFT_02013 0.60 +0.4
131,506 + H281DRAFT_02013 0.63 -1.3
131,506 + H281DRAFT_02013 0.63 -1.2
131,506 + H281DRAFT_02013 0.63 -0.3
131,646 + +0.1
131,646 + +3.0
131,646 + +0.4
131,646 + +0.4
131,647 - +1.0
131,647 - +0.2
131,647 - +1.5
131,647 - +0.7
131,647 - +0.6
131,647 - -0.4
131,679 + +1.0
131,692 + -0.5
131,732 - +0.7
131,756 + -0.1
131,757 - +1.2
131,819 - +1.6
131,901 + -1.1
131,943 + +0.1
131,943 + +0.9
131,943 + +0.2
131,943 + -0.3
131,943 + -0.8
131,944 - +0.8
131,944 - +1.2
131,944 - -0.5
132,057 + +0.4
132,057 + +0.4
132,058 - +1.2

Or see this region's nucleotide sequence