Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01341 and H281DRAFT_01342 are separated by 46 nucleotides H281DRAFT_01342 and H281DRAFT_01343 are separated by 40 nucleotides H281DRAFT_01343 and H281DRAFT_01344 are separated by 72 nucleotides H281DRAFT_01344 and H281DRAFT_01345 are separated by 28 nucleotides
H281DRAFT_01341: H281DRAFT_01341 - Protein of unknown function (DUF1488), at 182,802 to 183,080
_01341
H281DRAFT_01342: H281DRAFT_01342 - ABC-type phosphate/phosphonate transport system, substrate-binding protein, at 183,127 to 183,918
_01342
H281DRAFT_01343: H281DRAFT_01343 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 183,959 to 185,992
_01343
H281DRAFT_01344: H281DRAFT_01344 - methylglutaconyl-CoA hydratase, at 186,065 to 186,850
_01344
H281DRAFT_01345: H281DRAFT_01345 - 3-methylcrotonyl-CoA carboxylase beta subunit, at 186,879 to 188,486
_01345
Position (kb) 183
184
185
186 Strain fitness (log2 ratio) -2
-1
0
1 at 183.081 kb on + strand at 183.081 kb on + strand at 183.094 kb on + strand at 183.095 kb on - strand at 183.106 kb on + strand at 183.154 kb on + strand at 183.154 kb on + strand at 183.466 kb on + strand, within H281DRAFT_01342 at 183.503 kb on + strand, within H281DRAFT_01342 at 183.623 kb on + strand, within H281DRAFT_01342 at 183.689 kb on + strand, within H281DRAFT_01342 at 183.972 kb on - strand at 184.262 kb on - strand, within H281DRAFT_01343 at 184.304 kb on + strand, within H281DRAFT_01343 at 185.389 kb on - strand, within H281DRAFT_01343 at 186.843 kb on + strand at 186.905 kb on + strand at 186.906 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 183,081 + -0.3 183,081 + -0.2 183,094 + -1.4 183,095 - -0.4 183,106 + -0.3 183,154 + +0.5 183,154 + +1.0 183,466 + H281DRAFT_01342 0.43 -0.2 183,503 + H281DRAFT_01342 0.47 +0.1 183,623 + H281DRAFT_01342 0.63 -0.2 183,689 + H281DRAFT_01342 0.71 +0.2 183,972 - -0.1 184,262 - H281DRAFT_01343 0.15 -0.4 184,304 + H281DRAFT_01343 0.17 +1.0 185,389 - H281DRAFT_01343 0.70 -2.3 186,843 + -1.3 186,905 + -0.8 186,906 - +0.1
Or see this region's nucleotide sequence