Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_00648

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_00647 and H281DRAFT_00648 overlap by 4 nucleotidesH281DRAFT_00648 and H281DRAFT_00649 are separated by 62 nucleotides H281DRAFT_00647: H281DRAFT_00647 - 2-keto-3-deoxygluconate permease, at 724,964 to 725,944 _00647 H281DRAFT_00648: H281DRAFT_00648 - Uncharacterized conserved protein YgbK, DUF1537 family, at 725,941 to 727,239 _00648 H281DRAFT_00649: H281DRAFT_00649 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 727,302 to 728,303 _00649 Position (kb) 725 726 727 728Strain fitness (log2 ratio) -1 0 1 2at 724.963 kb on + strandat 724.963 kb on + strandat 724.974 kb on + strandat 724.974 kb on + strandat 724.975 kb on - strandat 725.035 kb on - strandat 725.035 kb on - strandat 725.035 kb on - strandat 725.135 kb on + strand, within H281DRAFT_00647at 725.177 kb on + strand, within H281DRAFT_00647at 725.232 kb on + strand, within H281DRAFT_00647at 725.396 kb on + strand, within H281DRAFT_00647at 725.687 kb on - strand, within H281DRAFT_00647at 725.696 kb on + strand, within H281DRAFT_00647at 725.697 kb on - strand, within H281DRAFT_00647at 725.752 kb on + strand, within H281DRAFT_00647at 725.808 kb on - strand, within H281DRAFT_00647at 725.821 kb on + strand, within H281DRAFT_00647at 726.176 kb on - strand, within H281DRAFT_00648at 727.077 kb on - strand, within H281DRAFT_00648at 727.178 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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724,963 + +0.9
724,963 + +0.4
724,974 + +0.6
724,974 + +1.0
724,975 - +0.6
725,035 - +0.2
725,035 - -0.2
725,035 - +1.2
725,135 + H281DRAFT_00647 0.17 +1.4
725,177 + H281DRAFT_00647 0.22 -0.0
725,232 + H281DRAFT_00647 0.27 +2.0
725,396 + H281DRAFT_00647 0.44 +0.6
725,687 - H281DRAFT_00647 0.74 -1.3
725,696 + H281DRAFT_00647 0.75 +0.4
725,697 - H281DRAFT_00647 0.75 -0.7
725,752 + H281DRAFT_00647 0.80 -0.0
725,808 - H281DRAFT_00647 0.86 +0.0
725,821 + H281DRAFT_00647 0.87 +0.2
726,176 - H281DRAFT_00648 0.18 -0.0
727,077 - H281DRAFT_00648 0.87 -0.7
727,178 - +0.6

Or see this region's nucleotide sequence