Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_00485

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_00482 and H281DRAFT_00483 are separated by 157 nucleotidesH281DRAFT_00483 and H281DRAFT_00484 are separated by 15 nucleotidesH281DRAFT_00484 and H281DRAFT_00485 are separated by 97 nucleotidesH281DRAFT_00485 and H281DRAFT_00486 are separated by 235 nucleotides H281DRAFT_00482: H281DRAFT_00482 - molecular chaperone HscA, at 539,384 to 541,255 _00482 H281DRAFT_00483: H281DRAFT_00483 - ferredoxin, 2Fe-2S, at 541,413 to 541,754 _00483 H281DRAFT_00484: H281DRAFT_00484 - FeS assembly protein IscX, at 541,770 to 541,967 _00484 H281DRAFT_00485: H281DRAFT_00485 - Glycine zipper 2TM domain-containing protein, at 542,065 to 542,829 _00485 H281DRAFT_00486: H281DRAFT_00486 - lysyl-tRNA synthetase, class II, at 543,065 to 544,675 _00486 Position (kb) 542 543Strain fitness (log2 ratio) -1 0 1at 541.753 kb on - strandat 541.753 kb on - strandat 541.963 kb on - strandat 542.135 kb on + strandat 542.183 kb on + strand, within H281DRAFT_00485at 542.202 kb on + strand, within H281DRAFT_00485at 542.430 kb on - strand, within H281DRAFT_00485at 542.465 kb on + strand, within H281DRAFT_00485at 542.860 kb on + strandat 542.860 kb on + strandat 542.861 kb on - strandat 542.861 kb on - strandat 542.862 kb on - strandat 542.909 kb on + strandat 542.960 kb on - strandat 542.969 kb on + strandat 542.970 kb on - strandat 543.060 kb on - strandat 543.060 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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541,753 - +1.0
541,753 - -0.8
541,963 - +1.2
542,135 + +0.3
542,183 + H281DRAFT_00485 0.15 -0.2
542,202 + H281DRAFT_00485 0.18 -0.1
542,430 - H281DRAFT_00485 0.48 +0.5
542,465 + H281DRAFT_00485 0.52 -0.1
542,860 + -0.8
542,860 + +0.8
542,861 - +0.2
542,861 - -0.2
542,862 - -0.7
542,909 + +0.4
542,960 - -0.1
542,969 + +0.5
542,970 - -0.2
543,060 - -0.7
543,060 - +1.3

Or see this region's nucleotide sequence