Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_00217

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_00215 and H281DRAFT_00216 are separated by 12 nucleotidesH281DRAFT_00216 and H281DRAFT_00217 are separated by 9 nucleotidesH281DRAFT_00217 and H281DRAFT_00218 are separated by 156 nucleotides H281DRAFT_00215: H281DRAFT_00215 - Uncharacterized membrane protein YccC, at 233,107 to 235,215 _00215 H281DRAFT_00216: H281DRAFT_00216 - putative endonuclease, at 235,228 to 235,554 _00216 H281DRAFT_00217: H281DRAFT_00217 - hypothetical protein, at 235,564 to 236,733 _00217 H281DRAFT_00218: H281DRAFT_00218 - NAD+ synthase (glutamine-hydrolysing), at 236,890 to 238,635 _00218 Position (kb) 235 236 237Strain fitness (log2 ratio) -1 0 1 2at 234.650 kb on - strand, within H281DRAFT_00215at 234.712 kb on + strand, within H281DRAFT_00215at 234.751 kb on + strand, within H281DRAFT_00215at 234.751 kb on + strand, within H281DRAFT_00215at 234.752 kb on - strand, within H281DRAFT_00215at 235.443 kb on - strand, within H281DRAFT_00216at 235.507 kb on - strand, within H281DRAFT_00216at 235.538 kb on + strandat 235.539 kb on - strandat 235.605 kb on - strandat 235.605 kb on - strandat 235.991 kb on + strand, within H281DRAFT_00217at 236.107 kb on + strand, within H281DRAFT_00217at 236.107 kb on + strand, within H281DRAFT_00217at 236.417 kb on + strand, within H281DRAFT_00217at 236.438 kb on + strand, within H281DRAFT_00217at 236.439 kb on - strand, within H281DRAFT_00217at 236.469 kb on - strand, within H281DRAFT_00217at 236.773 kb on + strandat 236.773 kb on + strandat 236.773 kb on + strandat 236.774 kb on - strandat 236.774 kb on - strandat 237.070 kb on + strand, within H281DRAFT_00218at 237.192 kb on + strand, within H281DRAFT_00218at 237.193 kb on - strand, within H281DRAFT_00218at 237.193 kb on - strand, within H281DRAFT_00218

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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234,650 - H281DRAFT_00215 0.73 +1.8
234,712 + H281DRAFT_00215 0.76 +0.2
234,751 + H281DRAFT_00215 0.78 -0.7
234,751 + H281DRAFT_00215 0.78 +0.5
234,752 - H281DRAFT_00215 0.78 +0.1
235,443 - H281DRAFT_00216 0.66 +0.4
235,507 - H281DRAFT_00216 0.85 +1.3
235,538 + -0.5
235,539 - +0.6
235,605 - +1.4
235,605 - -0.5
235,991 + H281DRAFT_00217 0.36 +1.3
236,107 + H281DRAFT_00217 0.46 -0.7
236,107 + H281DRAFT_00217 0.46 +0.9
236,417 + H281DRAFT_00217 0.73 -1.2
236,438 + H281DRAFT_00217 0.75 -0.6
236,439 - H281DRAFT_00217 0.75 +0.2
236,469 - H281DRAFT_00217 0.77 +0.2
236,773 + -0.2
236,773 + +0.5
236,773 + +0.4
236,774 - +1.7
236,774 - -0.0
237,070 + H281DRAFT_00218 0.10 +0.4
237,192 + H281DRAFT_00218 0.17 -0.3
237,193 - H281DRAFT_00218 0.17 +0.1
237,193 - H281DRAFT_00218 0.17 +0.1

Or see this region's nucleotide sequence