Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00008 and H281DRAFT_00009 overlap by 4 nucleotides H281DRAFT_00009 and H281DRAFT_00010 are separated by 300 nucleotides H281DRAFT_00010 and H281DRAFT_00011 overlap by 4 nucleotides H281DRAFT_00011 and H281DRAFT_00012 are separated by 1 nucleotides
H281DRAFT_00008: H281DRAFT_00008 - hypothetical protein, at 8,258 to 8,575
_00008
H281DRAFT_00009: H281DRAFT_00009 - RepB plasmid partitioning protein, at 8,572 to 9,186
_00009
H281DRAFT_00010: H281DRAFT_00010 - nitrile hydratase, at 9,487 to 10,146
_00010
H281DRAFT_00011: H281DRAFT_00011 - nitrile hydratase, at 10,143 to 10,814
_00011
H281DRAFT_00012: H281DRAFT_00012 - nitrile hydratase accessory protein, at 10,816 to 11,184
_00012
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 8.799 kb on - strand, within H281DRAFT_00009 at 8.799 kb on - strand, within H281DRAFT_00009 at 8.938 kb on + strand, within H281DRAFT_00009 at 8.954 kb on - strand, within H281DRAFT_00009 at 8.954 kb on - strand, within H281DRAFT_00009 at 8.957 kb on - strand, within H281DRAFT_00009 at 8.957 kb on - strand, within H281DRAFT_00009 at 9.020 kb on + strand, within H281DRAFT_00009 at 9.115 kb on + strand, within H281DRAFT_00009 at 9.220 kb on + strand at 9.221 kb on - strand at 9.238 kb on + strand at 9.268 kb on + strand at 9.269 kb on - strand at 9.310 kb on - strand at 9.450 kb on + strand at 9.450 kb on + strand at 9.608 kb on - strand, within H281DRAFT_00010 at 9.647 kb on - strand, within H281DRAFT_00010 at 9.686 kb on + strand, within H281DRAFT_00010 at 9.713 kb on - strand, within H281DRAFT_00010 at 9.834 kb on + strand, within H281DRAFT_00010 at 9.935 kb on + strand, within H281DRAFT_00010 at 10.142 kb on + strand at 10.142 kb on + strand at 10.142 kb on + strand at 10.143 kb on - strand at 10.143 kb on - strand at 10.143 kb on - strand at 10.143 kb on - strand at 10.143 kb on - strand at 10.143 kb on - strand at 10.143 kb on - strand at 10.330 kb on - strand, within H281DRAFT_00011 at 10.345 kb on - strand, within H281DRAFT_00011 at 10.347 kb on + strand, within H281DRAFT_00011 at 10.348 kb on - strand, within H281DRAFT_00011 at 10.444 kb on + strand, within H281DRAFT_00011 at 10.444 kb on + strand, within H281DRAFT_00011 at 10.602 kb on + strand, within H281DRAFT_00011 at 10.704 kb on - strand, within H281DRAFT_00011 at 10.704 kb on - strand, within H281DRAFT_00011 at 10.782 kb on + strand at 10.982 kb on - strand, within H281DRAFT_00012 at 11.041 kb on + strand, within H281DRAFT_00012 at 11.041 kb on + strand, within H281DRAFT_00012 at 11.042 kb on - strand, within H281DRAFT_00012 at 11.042 kb on - strand, within H281DRAFT_00012 at 11.045 kb on - strand, within H281DRAFT_00012
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 8,799 - H281DRAFT_00009 0.37 -0.1 8,799 - H281DRAFT_00009 0.37 +0.5 8,938 + H281DRAFT_00009 0.60 -0.7 8,954 - H281DRAFT_00009 0.62 -0.7 8,954 - H281DRAFT_00009 0.62 +0.6 8,957 - H281DRAFT_00009 0.63 +0.6 8,957 - H281DRAFT_00009 0.63 +1.2 9,020 + H281DRAFT_00009 0.73 +1.4 9,115 + H281DRAFT_00009 0.88 +0.7 9,220 + -0.0 9,221 - -1.4 9,238 + -0.1 9,268 + -1.2 9,269 - +0.0 9,310 - -0.4 9,450 + -0.2 9,450 + +0.7 9,608 - H281DRAFT_00010 0.18 +2.0 9,647 - H281DRAFT_00010 0.24 -1.9 9,686 + H281DRAFT_00010 0.30 +2.7 9,713 - H281DRAFT_00010 0.34 +0.1 9,834 + H281DRAFT_00010 0.53 +0.4 9,935 + H281DRAFT_00010 0.68 -0.2 10,142 + +0.1 10,142 + -0.3 10,142 + +1.0 10,143 - +0.1 10,143 - +0.3 10,143 - +0.6 10,143 - +0.1 10,143 - -0.9 10,143 - +0.4 10,143 - +0.3 10,330 - H281DRAFT_00011 0.28 +0.6 10,345 - H281DRAFT_00011 0.30 -1.8 10,347 + H281DRAFT_00011 0.30 +2.2 10,348 - H281DRAFT_00011 0.31 -0.8 10,444 + H281DRAFT_00011 0.45 -0.8 10,444 + H281DRAFT_00011 0.45 -0.6 10,602 + H281DRAFT_00011 0.68 -1.1 10,704 - H281DRAFT_00011 0.83 -0.5 10,704 - H281DRAFT_00011 0.83 -0.4 10,782 + +0.5 10,982 - H281DRAFT_00012 0.45 -0.7 11,041 + H281DRAFT_00012 0.61 +1.2 11,041 + H281DRAFT_00012 0.61 -0.2 11,042 - H281DRAFT_00012 0.61 +0.3 11,042 - H281DRAFT_00012 0.61 +0.2 11,045 - H281DRAFT_00012 0.62 +0.2
Or see this region's nucleotide sequence