Experiment: A22 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2117 and Shewana3_2118 are separated by 59 nucleotides Shewana3_2118 and Shewana3_2119 overlap by 8 nucleotides Shewana3_2119 and Shewana3_2120 are separated by 317 nucleotides
Shewana3_2117: Shewana3_2117 - PP-loop domain-containing protein (RefSeq), at 2,540,707 to 2,541,675
_2117
Shewana3_2118: Shewana3_2118 - hypothetical protein (RefSeq), at 2,541,735 to 2,542,358
_2118
Shewana3_2119: Shewana3_2119 - mannosyl-glycoprotein endo-beta-N-acetylglucosamidase (RefSeq), at 2,542,351 to 2,543,163
_2119
Shewana3_2120: Shewana3_2120 - hypothetical protein (RefSeq), at 2,543,481 to 2,544,404
_2120
Position (kb)
2542
2543
2544 Strain fitness (log2 ratio)
-1
0
1 at 2541.429 kb on + strand, within Shewana3_2117 at 2541.749 kb on + strand at 2541.823 kb on - strand, within Shewana3_2118 at 2541.856 kb on - strand, within Shewana3_2118 at 2541.876 kb on - strand, within Shewana3_2118 at 2542.008 kb on + strand, within Shewana3_2118 at 2542.053 kb on - strand, within Shewana3_2118 at 2542.568 kb on - strand, within Shewana3_2119 at 2542.601 kb on + strand, within Shewana3_2119 at 2542.774 kb on + strand, within Shewana3_2119 at 2542.846 kb on + strand, within Shewana3_2119 at 2543.124 kb on - strand at 2543.129 kb on - strand at 2543.285 kb on - strand at 2543.640 kb on + strand, within Shewana3_2120 at 2543.645 kb on + strand, within Shewana3_2120 at 2543.803 kb on + strand, within Shewana3_2120
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.05 mg/ml remove 2,541,429 + Shewana3_2117 0.75 +1.1 2,541,749 + +1.5 2,541,823 - Shewana3_2118 0.14 +0.0 2,541,856 - Shewana3_2118 0.19 -0.5 2,541,876 - Shewana3_2118 0.23 +0.7 2,542,008 + Shewana3_2118 0.44 +1.5 2,542,053 - Shewana3_2118 0.51 +0.9 2,542,568 - Shewana3_2119 0.27 +0.5 2,542,601 + Shewana3_2119 0.31 +1.5 2,542,774 + Shewana3_2119 0.52 +1.5 2,542,846 + Shewana3_2119 0.61 +1.5 2,543,124 - +1.5 2,543,129 - +1.5 2,543,285 - +1.5 2,543,640 + Shewana3_2120 0.17 -0.5 2,543,645 + Shewana3_2120 0.18 +1.5 2,543,803 + Shewana3_2120 0.35 +0.5
Or see this region's nucleotide sequence