Experiment: A22 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1545 and Shewana3_1546 are separated by 130 nucleotides Shewana3_1546 and Shewana3_1547 are separated by 321 nucleotides Shewana3_1547 and Shewana3_1548 are separated by 132 nucleotides Shewana3_1548 and Shewana3_1549 are separated by 486 nucleotides Shewana3_1549 and Shewana3_1550 are separated by 29 nucleotides
Shewana3_1545: Shewana3_1545 - hypothetical protein (RefSeq), at 1,802,402 to 1,803,313
_1545
Shewana3_1546: Shewana3_1546 - hypothetical protein (RefSeq), at 1,803,444 to 1,803,668
_1546
Shewana3_1547: Shewana3_1547 - hypothetical protein (RefSeq), at 1,803,990 to 1,804,082
_1547
Shewana3_1548: Shewana3_1548 - hypothetical protein (RefSeq), at 1,804,215 to 1,804,649
_1548
Shewana3_1549: Shewana3_1549 - transposase (RefSeq), at 1,805,136 to 1,805,531
_1549
Shewana3_1550: Shewana3_1550 - integrase catalytic subunit (RefSeq), at 1,805,561 to 1,806,364
_1550
Position (kb)
1804
1805 Strain fitness (log2 ratio)
-1
0
1 at 1803.300 kb on + strand at 1803.428 kb on + strand at 1803.451 kb on - strand at 1803.560 kb on - strand, within Shewana3_1546 at 1803.891 kb on + strand at 1803.899 kb on - strand at 1803.940 kb on + strand at 1804.311 kb on - strand, within Shewana3_1548 at 1804.319 kb on + strand, within Shewana3_1548 at 1804.495 kb on + strand, within Shewana3_1548 at 1805.071 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.05 mg/ml remove 1,803,300 + -1.3 1,803,428 + +1.3 1,803,451 - -0.1 1,803,560 - Shewana3_1546 0.52 +1.3 1,803,891 + -0.1 1,803,899 - +1.3 1,803,940 + -0.7 1,804,311 - Shewana3_1548 0.22 -0.3 1,804,319 + Shewana3_1548 0.24 +0.3 1,804,495 + Shewana3_1548 0.64 +0.3 1,805,071 + +1.3
Or see this region's nucleotide sequence