Experiment: A22 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0376 and Shewana3_0377 are separated by 8 nucleotides Shewana3_0377 and Shewana3_0378 are separated by 18 nucleotides Shewana3_0378 and Shewana3_0379 overlap by 4 nucleotides
Shewana3_0376: Shewana3_0376 - diguanylate cyclase/phosphodiesterase (RefSeq), at 417,068 to 418,987
_0376
Shewana3_0377: Shewana3_0377 - hypothetical protein (RefSeq), at 418,996 to 419,703
_0377
Shewana3_0378: Shewana3_0378 - OmpA/MotB domain-containing protein (RefSeq), at 419,722 to 420,330
_0378
Shewana3_0379: Shewana3_0379 - TolC family type I secretion outer membrane protein (RefSeq), at 420,327 to 421,742
_0379
Position (kb) 419
420
421 Strain fitness (log2 ratio) -2
-1
0
1 at 418.859 kb on + strand at 418.897 kb on + strand at 418.979 kb on - strand at 418.996 kb on - strand at 419.047 kb on - strand at 419.077 kb on + strand, within Shewana3_0377 at 419.190 kb on + strand, within Shewana3_0377 at 419.195 kb on + strand, within Shewana3_0377 at 419.199 kb on - strand, within Shewana3_0377 at 419.219 kb on + strand, within Shewana3_0377 at 419.273 kb on + strand, within Shewana3_0377 at 419.318 kb on - strand, within Shewana3_0377 at 419.558 kb on - strand, within Shewana3_0377 at 419.560 kb on - strand, within Shewana3_0377 at 419.685 kb on + strand at 419.763 kb on + strand at 419.888 kb on - strand, within Shewana3_0378 at 420.071 kb on + strand, within Shewana3_0378 at 420.154 kb on - strand, within Shewana3_0378 at 420.201 kb on - strand, within Shewana3_0378 at 420.386 kb on + strand at 420.646 kb on + strand, within Shewana3_0379 at 420.692 kb on + strand, within Shewana3_0379 at 421.273 kb on + strand, within Shewana3_0379 at 421.281 kb on - strand, within Shewana3_0379 at 421.302 kb on + strand, within Shewana3_0379
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.05 mg/ml remove 418,859 + +0.7 418,897 + +0.1 418,979 - +0.3 418,996 - +1.7 419,047 - -1.1 419,077 + Shewana3_0377 0.11 -0.9 419,190 + Shewana3_0377 0.27 -0.9 419,195 + Shewana3_0377 0.28 -1.0 419,199 - Shewana3_0377 0.29 -2.3 419,219 + Shewana3_0377 0.31 -2.0 419,273 + Shewana3_0377 0.39 -1.6 419,318 - Shewana3_0377 0.45 -1.9 419,558 - Shewana3_0377 0.79 -0.6 419,560 - Shewana3_0377 0.80 -0.2 419,685 + +0.0 419,763 + -0.6 419,888 - Shewana3_0378 0.27 -1.7 420,071 + Shewana3_0378 0.57 +0.1 420,154 - Shewana3_0378 0.71 -0.8 420,201 - Shewana3_0378 0.79 +0.1 420,386 + -1.3 420,646 + Shewana3_0379 0.23 -0.4 420,692 + Shewana3_0379 0.26 +1.7 421,273 + Shewana3_0379 0.67 +1.7 421,281 - Shewana3_0379 0.67 -0.6 421,302 + Shewana3_0379 0.69 -0.2
Or see this region's nucleotide sequence