Experiment: A22 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4141 and Shewana3_4142 are separated by 242 nucleotides Shewana3_4142 and Shewana3_4143 are separated by 83 nucleotides Shewana3_4143 and Shewana3_4144 are separated by 43 nucleotides Shewana3_4144 and Shewana3_4145 are separated by 36 nucleotides
Shewana3_4141: Shewana3_4141 - putative transposase (RefSeq), at 1,030 to 1,248
_4141
Shewana3_4142: Shewana3_4142 - IS4 family transposase (RefSeq), at 1,491 to 2,471
_4142
Shewana3_4143: Shewana3_4143 - hypothetical protein (RefSeq), at 2,555 to 2,836
_4143
Shewana3_4144: Shewana3_4144 - plastocyanin domain-containing protein (RefSeq), at 2,880 to 3,239
_4144
Shewana3_4145: Shewana3_4145 - copper-translocating P-type ATPase (RefSeq), at 3,276 to 5,612
_4145
Position (kb) 1
2
3 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 0.491 kb on - strand at 0.543 kb on + strand at 0.543 kb on + strand at 0.545 kb on + strand at 0.545 kb on + strand at 0.551 kb on - strand at 0.566 kb on - strand at 0.568 kb on + strand at 0.576 kb on - strand at 0.585 kb on - strand at 0.652 kb on + strand at 0.652 kb on + strand at 0.652 kb on + strand at 0.660 kb on - strand at 0.660 kb on - strand at 0.660 kb on - strand at 0.660 kb on - strand at 0.769 kb on + strand at 0.769 kb on + strand at 0.777 kb on - strand at 0.777 kb on - strand at 0.777 kb on - strand at 0.777 kb on - strand at 0.777 kb on - strand at 0.777 kb on - strand at 0.807 kb on - strand at 0.808 kb on - strand at 0.808 kb on - strand at 0.826 kb on - strand at 0.835 kb on - strand at 0.836 kb on - strand at 0.841 kb on + strand at 0.843 kb on - strand at 0.857 kb on + strand at 0.857 kb on + strand at 0.865 kb on - strand at 0.865 kb on - strand at 0.865 kb on - strand at 0.865 kb on - strand at 0.929 kb on - strand at 0.929 kb on - strand at 0.929 kb on - strand at 0.938 kb on - strand at 0.960 kb on + strand at 0.968 kb on - strand at 0.975 kb on + strand at 0.975 kb on + strand at 0.983 kb on - strand at 0.983 kb on - strand at 0.983 kb on - strand at 0.988 kb on + strand at 0.996 kb on - strand at 0.999 kb on - strand at 1.040 kb on - strand at 1.040 kb on - strand at 1.102 kb on + strand, within Shewana3_4141 at 1.138 kb on - strand, within Shewana3_4141 at 1.138 kb on - strand, within Shewana3_4141 at 1.156 kb on + strand, within Shewana3_4141 at 1.269 kb on + strand at 1.279 kb on + strand at 1.321 kb on - strand at 1.323 kb on + strand at 1.596 kb on - strand, within Shewana3_4142 at 1.689 kb on - strand, within Shewana3_4142 at 1.980 kb on - strand, within Shewana3_4142 at 2.130 kb on - strand, within Shewana3_4142 at 2.130 kb on - strand, within Shewana3_4142 at 2.181 kb on - strand, within Shewana3_4142 at 2.252 kb on + strand, within Shewana3_4142 at 2.276 kb on - strand, within Shewana3_4142 at 2.337 kb on - strand, within Shewana3_4142 at 2.352 kb on - strand, within Shewana3_4142 at 2.545 kb on - strand at 2.898 kb on + strand at 2.903 kb on + strand at 2.906 kb on - strand at 3.106 kb on + strand, within Shewana3_4144 at 3.209 kb on + strand at 3.231 kb on - strand at 3.321 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.05 mg/ml remove 491 - +0.6 543 + +1.6 543 + +1.6 545 + +1.6 545 + -0.8 551 - -1.4 566 - -0.7 568 + +1.6 576 - -0.4 585 - +0.6 652 + +1.6 652 + +1.2 652 + +1.6 660 - +0.6 660 - -1.2 660 - +2.1 660 - +1.6 769 + +1.6 769 + +0.2 777 - +1.6 777 - +0.1 777 - +1.6 777 - +1.6 777 - +0.2 777 - -1.2 807 - -0.8 808 - +1.6 808 - +1.6 826 - +1.6 835 - -0.0 836 - -1.6 841 + +1.6 843 - +1.6 857 + +0.8 857 + +0.4 865 - -0.2 865 - +1.6 865 - -0.0 865 - -0.2 929 - -0.1 929 - +0.2 929 - -1.2 938 - +0.4 960 + -0.2 968 - -1.8 975 + +1.6 975 + +1.6 983 - +0.2 983 - -0.9 983 - +0.9 988 + -0.8 996 - -0.7 999 - -0.0 1,040 - -0.6 1,040 - -0.9 1,102 + Shewana3_4141 0.33 +0.2 1,138 - Shewana3_4141 0.49 -0.0 1,138 - Shewana3_4141 0.49 -0.4 1,156 + Shewana3_4141 0.58 +0.6 1,269 + +1.6 1,279 + +1.6 1,321 - -0.2 1,323 + -1.0 1,596 - Shewana3_4142 0.11 -1.1 1,689 - Shewana3_4142 0.20 -0.9 1,980 - Shewana3_4142 0.50 +1.2 2,130 - Shewana3_4142 0.65 -0.9 2,130 - Shewana3_4142 0.65 -0.6 2,181 - Shewana3_4142 0.70 +0.9 2,252 + Shewana3_4142 0.78 -0.6 2,276 - Shewana3_4142 0.80 -0.6 2,337 - Shewana3_4142 0.86 -0.6 2,352 - Shewana3_4142 0.88 -0.0 2,545 - +3.9 2,898 + -1.3 2,903 + -0.7 2,906 - +0.0 3,106 + Shewana3_4144 0.63 -3.5 3,209 + -0.4 3,231 - -0.2 3,321 - +0.9
Or see this region's nucleotide sequence