Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS37475 and RR42_RS37480 are separated by 135 nucleotides RR42_RS37480 and RR42_RS37485 are separated by 79 nucleotides
RR42_RS37475: RR42_RS37475 - peptide chain release factor 3, at 3,886,294 to 3,887,868
_RS37475
RR42_RS37480: RR42_RS37480 - membrane protein, at 3,888,004 to 3,888,969
_RS37480
RR42_RS37485: RR42_RS37485 - membrane protein, at 3,889,049 to 3,889,216
_RS37485
Position (kb)
3888
3889 Strain fitness (log2 ratio)
-2
-1
0
1 at 3887.120 kb on + strand, within RR42_RS37475 at 3887.121 kb on - strand, within RR42_RS37475 at 3887.327 kb on + strand, within RR42_RS37475 at 3887.327 kb on + strand, within RR42_RS37475 at 3887.327 kb on + strand, within RR42_RS37475 at 3887.327 kb on + strand, within RR42_RS37475 at 3887.328 kb on - strand, within RR42_RS37475 at 3887.454 kb on + strand, within RR42_RS37475 at 3887.454 kb on + strand, within RR42_RS37475 at 3887.455 kb on - strand, within RR42_RS37475 at 3887.576 kb on + strand, within RR42_RS37475 at 3887.576 kb on + strand, within RR42_RS37475 at 3887.576 kb on + strand, within RR42_RS37475 at 3887.576 kb on + strand, within RR42_RS37475 at 3887.577 kb on - strand, within RR42_RS37475 at 3887.577 kb on - strand, within RR42_RS37475 at 3887.577 kb on - strand, within RR42_RS37475 at 3887.800 kb on - strand at 3887.855 kb on - strand at 3888.086 kb on + strand at 3888.195 kb on + strand, within RR42_RS37480 at 3888.195 kb on + strand, within RR42_RS37480 at 3888.196 kb on - strand, within RR42_RS37480 at 3888.206 kb on + strand, within RR42_RS37480 at 3888.813 kb on + strand, within RR42_RS37480 at 3888.813 kb on + strand, within RR42_RS37480 at 3888.814 kb on - strand, within RR42_RS37480 at 3888.814 kb on - strand, within RR42_RS37480 at 3888.814 kb on - strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.815 kb on + strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3888.816 kb on - strand, within RR42_RS37480 at 3889.029 kb on - strand at 3889.029 kb on - strand at 3889.051 kb on - strand at 3889.206 kb on + strand at 3889.207 kb on - strand at 3889.272 kb on + strand at 3889.272 kb on + strand at 3889.272 kb on + strand at 3889.273 kb on - strand at 3889.273 kb on - strand at 3889.354 kb on - strand at 3889.390 kb on + strand at 3889.773 kb on + strand at 3889.774 kb on - strand at 3889.826 kb on + strand at 3889.845 kb on + strand at 3889.845 kb on + strand at 3889.845 kb on + strand at 3889.845 kb on + strand at 3889.846 kb on - strand at 3889.901 kb on + strand at 3889.902 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 3,887,120 + RR42_RS37475 0.52 +1.1 3,887,121 - RR42_RS37475 0.53 -0.2 3,887,327 + RR42_RS37475 0.66 -2.0 3,887,327 + RR42_RS37475 0.66 -0.4 3,887,327 + RR42_RS37475 0.66 -1.8 3,887,327 + RR42_RS37475 0.66 -0.5 3,887,328 - RR42_RS37475 0.66 -0.3 3,887,454 + RR42_RS37475 0.74 -0.5 3,887,454 + RR42_RS37475 0.74 -1.0 3,887,455 - RR42_RS37475 0.74 +0.1 3,887,576 + RR42_RS37475 0.81 +0.3 3,887,576 + RR42_RS37475 0.81 -1.2 3,887,576 + RR42_RS37475 0.81 -0.8 3,887,576 + RR42_RS37475 0.81 -2.4 3,887,577 - RR42_RS37475 0.81 +0.2 3,887,577 - RR42_RS37475 0.81 -0.7 3,887,577 - RR42_RS37475 0.81 -1.3 3,887,800 - -0.4 3,887,855 - -0.2 3,888,086 + -0.8 3,888,195 + RR42_RS37480 0.20 -1.5 3,888,195 + RR42_RS37480 0.20 -0.1 3,888,196 - RR42_RS37480 0.20 +1.0 3,888,206 + RR42_RS37480 0.21 -0.5 3,888,813 + RR42_RS37480 0.84 +0.2 3,888,813 + RR42_RS37480 0.84 -0.1 3,888,814 - RR42_RS37480 0.84 +0.2 3,888,814 - RR42_RS37480 0.84 +0.2 3,888,814 - RR42_RS37480 0.84 -1.3 3,888,815 + RR42_RS37480 0.84 -1.0 3,888,815 + RR42_RS37480 0.84 -1.6 3,888,815 + RR42_RS37480 0.84 -0.4 3,888,815 + RR42_RS37480 0.84 -0.1 3,888,815 + RR42_RS37480 0.84 +0.7 3,888,815 + RR42_RS37480 0.84 +0.3 3,888,815 + RR42_RS37480 0.84 +0.1 3,888,815 + RR42_RS37480 0.84 -0.5 3,888,816 - RR42_RS37480 0.84 -2.3 3,888,816 - RR42_RS37480 0.84 +0.2 3,888,816 - RR42_RS37480 0.84 -0.6 3,888,816 - RR42_RS37480 0.84 +0.5 3,888,816 - RR42_RS37480 0.84 -0.7 3,888,816 - RR42_RS37480 0.84 -0.3 3,888,816 - RR42_RS37480 0.84 -2.4 3,888,816 - RR42_RS37480 0.84 +0.2 3,888,816 - RR42_RS37480 0.84 -0.1 3,889,029 - -0.7 3,889,029 - +0.6 3,889,051 - +0.1 3,889,206 + +0.4 3,889,207 - -0.7 3,889,272 + +0.5 3,889,272 + -0.1 3,889,272 + -0.4 3,889,273 - +0.8 3,889,273 - -0.2 3,889,354 - +0.1 3,889,390 + -0.3 3,889,773 + +0.1 3,889,774 - -2.4 3,889,826 + +0.1 3,889,845 + +0.4 3,889,845 + +0.0 3,889,845 + +0.4 3,889,845 + +1.3 3,889,846 - -0.4 3,889,901 + -0.7 3,889,902 - +0.6
Or see this region's nucleotide sequence