Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35910 and RR42_RS35915 are separated by 223 nucleotides RR42_RS35915 and RR42_RS35920 are separated by 100 nucleotides RR42_RS35920 and RR42_RS35925 are separated by 20 nucleotides
RR42_RS35910: RR42_RS35910 - short-chain dehydrogenase, at 3,503,423 to 3,504,154
_RS35910
RR42_RS35915: RR42_RS35915 - beta-ketoadipyl CoA thiolase, at 3,504,378 to 3,505,580
_RS35915
RR42_RS35920: RR42_RS35920 - 3-oxoadipate CoA-transferase, at 3,505,681 to 3,506,328
_RS35920
RR42_RS35925: RR42_RS35925 - 3-oxoadipate CoA-transferase, at 3,506,349 to 3,507,032
_RS35925
Position (kb)
3504
3505
3506 Strain fitness (log2 ratio)
-1
0
1
2 at 3503.425 kb on - strand at 3503.463 kb on + strand at 3503.463 kb on + strand at 3503.463 kb on + strand at 3503.463 kb on + strand at 3503.463 kb on + strand at 3503.463 kb on + strand at 3503.463 kb on + strand at 3503.464 kb on - strand at 3503.464 kb on - strand at 3503.464 kb on - strand at 3503.464 kb on - strand at 3503.464 kb on - strand at 3503.464 kb on - strand at 3503.727 kb on + strand, within RR42_RS35910 at 3503.727 kb on + strand, within RR42_RS35910 at 3503.728 kb on - strand, within RR42_RS35910 at 3503.728 kb on - strand, within RR42_RS35910 at 3503.728 kb on - strand, within RR42_RS35910 at 3503.862 kb on + strand, within RR42_RS35910 at 3503.862 kb on + strand, within RR42_RS35910 at 3503.862 kb on + strand, within RR42_RS35910 at 3503.862 kb on + strand, within RR42_RS35910 at 3503.863 kb on - strand, within RR42_RS35910 at 3503.863 kb on - strand, within RR42_RS35910 at 3503.863 kb on - strand, within RR42_RS35910 at 3504.046 kb on - strand, within RR42_RS35910 at 3504.046 kb on - strand, within RR42_RS35910 at 3504.046 kb on - strand, within RR42_RS35910 at 3504.046 kb on - strand, within RR42_RS35910 at 3504.159 kb on + strand at 3504.159 kb on + strand at 3504.160 kb on - strand at 3504.160 kb on - strand at 3504.160 kb on - strand at 3504.160 kb on - strand at 3504.287 kb on + strand at 3504.311 kb on + strand at 3504.469 kb on + strand at 3504.469 kb on + strand at 3504.469 kb on + strand at 3504.469 kb on + strand at 3504.469 kb on + strand at 3504.470 kb on - strand at 3504.470 kb on - strand at 3505.060 kb on + strand, within RR42_RS35915 at 3505.061 kb on - strand, within RR42_RS35915 at 3505.373 kb on + strand, within RR42_RS35915 at 3505.374 kb on - strand, within RR42_RS35915 at 3505.380 kb on + strand, within RR42_RS35915 at 3505.420 kb on + strand, within RR42_RS35915 at 3505.420 kb on + strand, within RR42_RS35915 at 3505.421 kb on - strand, within RR42_RS35915 at 3505.679 kb on - strand at 3505.807 kb on - strand, within RR42_RS35920 at 3505.808 kb on + strand, within RR42_RS35920 at 3505.808 kb on + strand, within RR42_RS35920 at 3505.808 kb on + strand, within RR42_RS35920 at 3505.808 kb on + strand, within RR42_RS35920 at 3505.809 kb on - strand, within RR42_RS35920 at 3505.809 kb on - strand, within RR42_RS35920 at 3505.809 kb on - strand, within RR42_RS35920 at 3505.809 kb on - strand, within RR42_RS35920 at 3505.809 kb on - strand, within RR42_RS35920 at 3505.809 kb on - strand, within RR42_RS35920 at 3505.809 kb on - strand, within RR42_RS35920 at 3505.809 kb on - strand, within RR42_RS35920 at 3505.809 kb on - strand, within RR42_RS35920 at 3505.809 kb on - strand, within RR42_RS35920 at 3505.974 kb on + strand, within RR42_RS35920 at 3506.081 kb on + strand, within RR42_RS35920 at 3506.081 kb on + strand, within RR42_RS35920 at 3506.081 kb on + strand, within RR42_RS35920 at 3506.082 kb on - strand, within RR42_RS35920 at 3506.328 kb on + strand at 3506.328 kb on + strand at 3506.328 kb on + strand at 3506.329 kb on - strand at 3506.329 kb on - strand at 3506.339 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 3,503,425 - +0.5 3,503,463 + +0.6 3,503,463 + +0.1 3,503,463 + +0.5 3,503,463 + -1.1 3,503,463 + +0.1 3,503,463 + +1.3 3,503,463 + +0.8 3,503,464 - +0.7 3,503,464 - +0.3 3,503,464 - +2.6 3,503,464 - +1.3 3,503,464 - +0.4 3,503,464 - +0.8 3,503,727 + RR42_RS35910 0.42 +0.8 3,503,727 + RR42_RS35910 0.42 -0.1 3,503,728 - RR42_RS35910 0.42 -0.0 3,503,728 - RR42_RS35910 0.42 -0.2 3,503,728 - RR42_RS35910 0.42 +0.2 3,503,862 + RR42_RS35910 0.60 +0.0 3,503,862 + RR42_RS35910 0.60 +0.0 3,503,862 + RR42_RS35910 0.60 -0.3 3,503,862 + RR42_RS35910 0.60 +0.8 3,503,863 - RR42_RS35910 0.60 +0.7 3,503,863 - RR42_RS35910 0.60 +0.5 3,503,863 - RR42_RS35910 0.60 +0.2 3,504,046 - RR42_RS35910 0.85 +0.9 3,504,046 - RR42_RS35910 0.85 +0.6 3,504,046 - RR42_RS35910 0.85 +1.2 3,504,046 - RR42_RS35910 0.85 -0.1 3,504,159 + -0.3 3,504,159 + -0.1 3,504,160 - +0.4 3,504,160 - +0.4 3,504,160 - +2.1 3,504,160 - +0.8 3,504,287 + -0.4 3,504,311 + -0.7 3,504,469 + +0.6 3,504,469 + +0.7 3,504,469 + -0.2 3,504,469 + -0.2 3,504,469 + -1.4 3,504,470 - -0.5 3,504,470 - +0.3 3,505,060 + RR42_RS35915 0.57 +1.0 3,505,061 - RR42_RS35915 0.57 -1.7 3,505,373 + RR42_RS35915 0.83 +0.3 3,505,374 - RR42_RS35915 0.83 -0.7 3,505,380 + RR42_RS35915 0.83 +0.5 3,505,420 + RR42_RS35915 0.87 +0.9 3,505,420 + RR42_RS35915 0.87 -0.5 3,505,421 - RR42_RS35915 0.87 -0.1 3,505,679 - -0.4 3,505,807 - RR42_RS35920 0.19 +1.0 3,505,808 + RR42_RS35920 0.20 -0.5 3,505,808 + RR42_RS35920 0.20 +0.5 3,505,808 + RR42_RS35920 0.20 +0.9 3,505,808 + RR42_RS35920 0.20 -0.1 3,505,809 - RR42_RS35920 0.20 +0.1 3,505,809 - RR42_RS35920 0.20 -0.1 3,505,809 - RR42_RS35920 0.20 +0.5 3,505,809 - RR42_RS35920 0.20 +0.7 3,505,809 - RR42_RS35920 0.20 +0.6 3,505,809 - RR42_RS35920 0.20 +0.1 3,505,809 - RR42_RS35920 0.20 +0.7 3,505,809 - RR42_RS35920 0.20 +0.7 3,505,809 - RR42_RS35920 0.20 +0.8 3,505,809 - RR42_RS35920 0.20 +0.2 3,505,974 + RR42_RS35920 0.45 +0.2 3,506,081 + RR42_RS35920 0.62 -0.4 3,506,081 + RR42_RS35920 0.62 +0.3 3,506,081 + RR42_RS35920 0.62 +0.1 3,506,082 - RR42_RS35920 0.62 +0.4 3,506,328 + +0.4 3,506,328 + +0.2 3,506,328 + +0.1 3,506,329 - +0.2 3,506,329 - -0.2 3,506,339 + -0.2
Or see this region's nucleotide sequence