Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32260 and RR42_RS32265 are separated by 93 nucleotides RR42_RS32265 and RR42_RS32270 are separated by 253 nucleotides RR42_RS32270 and RR42_RS32275 are separated by 144 nucleotides
RR42_RS32260: RR42_RS32260 - squalene--hopene cyclase, at 2,625,018 to 2,627,033
_RS32260
RR42_RS32265: RR42_RS32265 - phytoene synthase, at 2,627,127 to 2,627,975
_RS32265
RR42_RS32270: RR42_RS32270 - hypothetical protein, at 2,628,229 to 2,628,489
_RS32270
RR42_RS32275: RR42_RS32275 - gamma-glutamyltransferase, at 2,628,634 to 2,630,226
_RS32275
Position (kb)
2627
2628 Strain fitness (log2 ratio)
-1
0
1
2 at 2626.287 kb on + strand, within RR42_RS32260 at 2626.492 kb on + strand, within RR42_RS32260 at 2626.492 kb on + strand at 2626.492 kb on + strand, within RR42_RS32260 at 2626.493 kb on - strand, within RR42_RS32260 at 2626.493 kb on - strand, within RR42_RS32260 at 2626.493 kb on - strand, within RR42_RS32260 at 2626.493 kb on - strand, within RR42_RS32260 at 2626.608 kb on - strand, within RR42_RS32260 at 2626.608 kb on - strand, within RR42_RS32260 at 2626.711 kb on + strand, within RR42_RS32260 at 2626.711 kb on + strand, within RR42_RS32260 at 2626.712 kb on - strand, within RR42_RS32260 at 2626.806 kb on - strand, within RR42_RS32260 at 2626.843 kb on + strand at 2626.844 kb on - strand at 2626.844 kb on - strand at 2626.861 kb on + strand at 2626.861 kb on + strand at 2627.016 kb on + strand at 2627.066 kb on + strand at 2627.067 kb on - strand at 2627.157 kb on + strand at 2627.240 kb on - strand, within RR42_RS32265 at 2627.314 kb on + strand, within RR42_RS32265 at 2627.315 kb on - strand, within RR42_RS32265 at 2627.317 kb on + strand, within RR42_RS32265 at 2627.317 kb on + strand, within RR42_RS32265 at 2627.318 kb on - strand, within RR42_RS32265 at 2627.434 kb on + strand, within RR42_RS32265 at 2627.435 kb on - strand, within RR42_RS32265 at 2627.435 kb on - strand, within RR42_RS32265 at 2627.521 kb on - strand, within RR42_RS32265 at 2627.593 kb on + strand, within RR42_RS32265 at 2627.593 kb on + strand, within RR42_RS32265 at 2627.594 kb on - strand, within RR42_RS32265 at 2627.594 kb on - strand, within RR42_RS32265 at 2627.594 kb on - strand, within RR42_RS32265 at 2627.601 kb on + strand, within RR42_RS32265 at 2627.601 kb on + strand, within RR42_RS32265 at 2627.601 kb on + strand, within RR42_RS32265 at 2627.602 kb on - strand, within RR42_RS32265 at 2627.607 kb on - strand, within RR42_RS32265 at 2627.746 kb on + strand, within RR42_RS32265 at 2627.747 kb on - strand, within RR42_RS32265 at 2627.747 kb on - strand, within RR42_RS32265 at 2627.822 kb on + strand, within RR42_RS32265 at 2627.822 kb on + strand, within RR42_RS32265 at 2627.823 kb on - strand, within RR42_RS32265 at 2627.823 kb on - strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.848 kb on + strand, within RR42_RS32265 at 2627.849 kb on - strand, within RR42_RS32265 at 2627.849 kb on - strand, within RR42_RS32265 at 2627.849 kb on - strand, within RR42_RS32265 at 2627.900 kb on + strand at 2627.977 kb on + strand at 2627.977 kb on + strand at 2627.978 kb on - strand at 2627.978 kb on - strand at 2628.021 kb on - strand at 2628.021 kb on - strand at 2628.021 kb on - strand at 2628.086 kb on + strand at 2628.086 kb on + strand at 2628.088 kb on + strand at 2628.088 kb on + strand at 2628.088 kb on + strand at 2628.088 kb on + strand at 2628.088 kb on + strand at 2628.088 kb on + strand at 2628.088 kb on + strand at 2628.089 kb on - strand at 2628.089 kb on - strand at 2628.089 kb on - strand at 2628.089 kb on - strand at 2628.230 kb on + strand at 2628.231 kb on - strand at 2628.234 kb on + strand at 2628.290 kb on + strand, within RR42_RS32270 at 2628.308 kb on + strand, within RR42_RS32270 at 2628.478 kb on + strand at 2628.478 kb on + strand at 2628.478 kb on + strand at 2628.479 kb on - strand at 2628.479 kb on - strand at 2628.521 kb on + strand at 2628.532 kb on + strand at 2628.532 kb on + strand at 2628.532 kb on + strand at 2628.532 kb on + strand at 2628.532 kb on + strand at 2628.542 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 2,626,287 + RR42_RS32260 0.63 -0.2 2,626,492 + RR42_RS32260 0.73 +0.3 2,626,492 + +0.5 2,626,492 + RR42_RS32260 0.73 -1.0 2,626,493 - RR42_RS32260 0.73 +1.1 2,626,493 - RR42_RS32260 0.73 -0.3 2,626,493 - RR42_RS32260 0.73 -0.6 2,626,493 - RR42_RS32260 0.73 -0.0 2,626,608 - RR42_RS32260 0.79 -0.7 2,626,608 - RR42_RS32260 0.79 +0.5 2,626,711 + RR42_RS32260 0.84 -1.4 2,626,711 + RR42_RS32260 0.84 -0.7 2,626,712 - RR42_RS32260 0.84 +0.2 2,626,806 - RR42_RS32260 0.89 +0.0 2,626,843 + +0.1 2,626,844 - -0.7 2,626,844 - -0.5 2,626,861 + +1.3 2,626,861 + +0.4 2,627,016 + -0.1 2,627,066 + +0.5 2,627,067 - +0.3 2,627,157 + +0.2 2,627,240 - RR42_RS32265 0.13 +0.4 2,627,314 + RR42_RS32265 0.22 +0.8 2,627,315 - RR42_RS32265 0.22 +0.9 2,627,317 + RR42_RS32265 0.22 -1.0 2,627,317 + RR42_RS32265 0.22 +0.9 2,627,318 - RR42_RS32265 0.22 +0.1 2,627,434 + RR42_RS32265 0.36 -0.5 2,627,435 - RR42_RS32265 0.36 +0.9 2,627,435 - RR42_RS32265 0.36 +1.1 2,627,521 - RR42_RS32265 0.46 +1.9 2,627,593 + RR42_RS32265 0.55 +1.6 2,627,593 + RR42_RS32265 0.55 +0.6 2,627,594 - RR42_RS32265 0.55 +0.6 2,627,594 - RR42_RS32265 0.55 +0.6 2,627,594 - RR42_RS32265 0.55 -0.3 2,627,601 + RR42_RS32265 0.56 +0.4 2,627,601 + RR42_RS32265 0.56 +0.6 2,627,601 + RR42_RS32265 0.56 +0.0 2,627,602 - RR42_RS32265 0.56 +0.5 2,627,607 - RR42_RS32265 0.57 -0.3 2,627,746 + RR42_RS32265 0.73 +0.9 2,627,747 - RR42_RS32265 0.73 +1.6 2,627,747 - RR42_RS32265 0.73 +0.5 2,627,822 + RR42_RS32265 0.82 +0.6 2,627,822 + RR42_RS32265 0.82 +0.4 2,627,823 - RR42_RS32265 0.82 +0.6 2,627,823 - RR42_RS32265 0.82 +1.3 2,627,848 + RR42_RS32265 0.85 +0.9 2,627,848 + RR42_RS32265 0.85 +0.5 2,627,848 + RR42_RS32265 0.85 +1.3 2,627,848 + RR42_RS32265 0.85 +0.6 2,627,849 - RR42_RS32265 0.85 +0.8 2,627,849 - RR42_RS32265 0.85 +1.2 2,627,849 - RR42_RS32265 0.85 +1.2 2,627,900 + +0.1 2,627,977 + +0.5 2,627,977 + +0.9 2,627,978 - -1.5 2,627,978 - +0.2 2,628,021 - +0.4 2,628,021 - -0.1 2,628,021 - +0.9 2,628,086 + +0.0 2,628,086 + +0.8 2,628,088 + -0.5 2,628,088 + -0.3 2,628,088 + -0.1 2,628,088 + +1.4 2,628,088 + +2.5 2,628,088 + +0.2 2,628,088 + +0.4 2,628,089 - +1.0 2,628,089 - +0.3 2,628,089 - +0.5 2,628,089 - +0.5 2,628,230 + +0.4 2,628,231 - +0.3 2,628,234 + +0.0 2,628,290 + RR42_RS32270 0.23 +0.4 2,628,308 + RR42_RS32270 0.30 -0.7 2,628,478 + +0.7 2,628,478 + +0.1 2,628,478 + +0.2 2,628,479 - -0.5 2,628,479 - +0.9 2,628,521 + -0.5 2,628,532 + -0.3 2,628,532 + +0.6 2,628,532 + +0.7 2,628,532 + +0.3 2,628,532 + -0.1 2,628,542 + -0.0
Or see this region's nucleotide sequence