Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27920 and RR42_RS27925 are separated by 47 nucleotides RR42_RS27925 and RR42_RS27930 are separated by 135 nucleotides
RR42_RS27920: RR42_RS27920 - hypothetical protein, at 1,627,592 to 1,628,158
_RS27920
RR42_RS27925: RR42_RS27925 - hypothetical protein, at 1,628,206 to 1,628,637
_RS27925
RR42_RS27930: RR42_RS27930 - amidohydrolase, at 1,628,773 to 1,629,936
_RS27930
Position (kb)
1628
1629
1630 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1627.863 kb on + strand, within RR42_RS27920 at 1627.864 kb on - strand, within RR42_RS27920 at 1627.864 kb on - strand, within RR42_RS27920 at 1627.925 kb on + strand, within RR42_RS27920 at 1627.925 kb on + strand, within RR42_RS27920 at 1627.926 kb on - strand, within RR42_RS27920 at 1628.005 kb on + strand, within RR42_RS27920 at 1628.006 kb on - strand, within RR42_RS27920 at 1628.009 kb on - strand, within RR42_RS27920 at 1628.009 kb on - strand, within RR42_RS27920 at 1628.013 kb on + strand, within RR42_RS27920 at 1628.013 kb on + strand, within RR42_RS27920 at 1628.013 kb on + strand, within RR42_RS27920 at 1628.013 kb on + strand, within RR42_RS27920 at 1628.014 kb on - strand, within RR42_RS27920 at 1628.020 kb on - strand, within RR42_RS27920 at 1628.151 kb on - strand at 1628.154 kb on - strand at 1628.154 kb on - strand at 1628.180 kb on + strand at 1628.231 kb on + strand at 1628.254 kb on + strand, within RR42_RS27925 at 1628.254 kb on + strand, within RR42_RS27925 at 1628.255 kb on - strand, within RR42_RS27925 at 1628.358 kb on + strand, within RR42_RS27925 at 1628.359 kb on - strand, within RR42_RS27925 at 1628.390 kb on + strand, within RR42_RS27925 at 1628.391 kb on - strand, within RR42_RS27925 at 1628.391 kb on - strand, within RR42_RS27925 at 1628.487 kb on + strand, within RR42_RS27925 at 1628.488 kb on - strand, within RR42_RS27925 at 1628.679 kb on + strand at 1628.786 kb on + strand at 1628.787 kb on - strand at 1628.787 kb on - strand at 1628.909 kb on + strand, within RR42_RS27930 at 1628.910 kb on - strand, within RR42_RS27930 at 1628.910 kb on - strand, within RR42_RS27930 at 1629.046 kb on - strand, within RR42_RS27930 at 1629.075 kb on - strand, within RR42_RS27930 at 1629.215 kb on + strand, within RR42_RS27930 at 1629.215 kb on + strand, within RR42_RS27930 at 1629.216 kb on - strand, within RR42_RS27930 at 1629.471 kb on - strand, within RR42_RS27930 at 1629.664 kb on + strand, within RR42_RS27930 at 1629.677 kb on + strand, within RR42_RS27930 at 1629.804 kb on - strand, within RR42_RS27930 at 1629.804 kb on - strand, within RR42_RS27930 at 1630.082 kb on - strand at 1630.082 kb on - strand at 1630.174 kb on + strand at 1630.174 kb on + strand at 1630.175 kb on - strand at 1630.175 kb on - strand at 1630.175 kb on - strand at 1630.175 kb on - strand at 1630.516 kb on + strand at 1630.516 kb on + strand at 1630.517 kb on - strand at 1630.517 kb on - strand at 1630.517 kb on - strand at 1630.517 kb on - strand at 1630.531 kb on + strand at 1630.531 kb on + strand at 1630.531 kb on + strand at 1630.531 kb on + strand at 1630.531 kb on + strand at 1630.532 kb on - strand at 1630.573 kb on - strand at 1630.661 kb on + strand at 1630.662 kb on - strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.663 kb on + strand at 1630.664 kb on - strand at 1630.664 kb on - strand at 1630.664 kb on - strand at 1630.664 kb on - strand at 1630.664 kb on - strand at 1630.664 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 1,627,863 + RR42_RS27920 0.48 -0.3 1,627,864 - RR42_RS27920 0.48 +0.3 1,627,864 - RR42_RS27920 0.48 -0.8 1,627,925 + RR42_RS27920 0.59 -0.2 1,627,925 + RR42_RS27920 0.59 +0.1 1,627,926 - RR42_RS27920 0.59 +0.0 1,628,005 + RR42_RS27920 0.73 +0.2 1,628,006 - RR42_RS27920 0.73 -1.0 1,628,009 - RR42_RS27920 0.74 -0.1 1,628,009 - RR42_RS27920 0.74 -0.3 1,628,013 + RR42_RS27920 0.74 -0.9 1,628,013 + RR42_RS27920 0.74 -0.8 1,628,013 + RR42_RS27920 0.74 +0.6 1,628,013 + RR42_RS27920 0.74 -0.1 1,628,014 - RR42_RS27920 0.74 +0.2 1,628,020 - RR42_RS27920 0.75 -0.1 1,628,151 - -1.1 1,628,154 - -0.4 1,628,154 - +0.6 1,628,180 + +0.5 1,628,231 + +0.5 1,628,254 + RR42_RS27925 0.11 -0.2 1,628,254 + RR42_RS27925 0.11 +3.9 1,628,255 - RR42_RS27925 0.11 -1.5 1,628,358 + RR42_RS27925 0.35 -2.8 1,628,359 - RR42_RS27925 0.35 -0.8 1,628,390 + RR42_RS27925 0.43 -0.4 1,628,391 - RR42_RS27925 0.43 -0.4 1,628,391 - RR42_RS27925 0.43 -0.4 1,628,487 + RR42_RS27925 0.65 -0.0 1,628,488 - RR42_RS27925 0.65 -1.0 1,628,679 + +0.1 1,628,786 + -1.0 1,628,787 - -0.8 1,628,787 - -0.5 1,628,909 + RR42_RS27930 0.12 +0.1 1,628,910 - RR42_RS27930 0.12 +0.5 1,628,910 - RR42_RS27930 0.12 +0.0 1,629,046 - RR42_RS27930 0.23 -1.0 1,629,075 - RR42_RS27930 0.26 -0.4 1,629,215 + RR42_RS27930 0.38 +0.2 1,629,215 + RR42_RS27930 0.38 -0.3 1,629,216 - RR42_RS27930 0.38 +0.7 1,629,471 - RR42_RS27930 0.60 +0.2 1,629,664 + RR42_RS27930 0.77 -0.1 1,629,677 + RR42_RS27930 0.78 +0.1 1,629,804 - RR42_RS27930 0.89 +0.5 1,629,804 - RR42_RS27930 0.89 -0.3 1,630,082 - +0.3 1,630,082 - -0.3 1,630,174 + -0.6 1,630,174 + -1.1 1,630,175 - -1.0 1,630,175 - -0.2 1,630,175 - +0.7 1,630,175 - -0.2 1,630,516 + -0.0 1,630,516 + +0.0 1,630,517 - +0.1 1,630,517 - +0.8 1,630,517 - +0.6 1,630,517 - -1.1 1,630,531 + -0.4 1,630,531 + -0.4 1,630,531 + -1.8 1,630,531 + -0.2 1,630,531 + -0.2 1,630,532 - +0.1 1,630,573 - -0.4 1,630,661 + +0.2 1,630,662 - -0.9 1,630,663 + -1.7 1,630,663 + +0.2 1,630,663 + -0.2 1,630,663 + -1.1 1,630,663 + +0.1 1,630,663 + +1.0 1,630,663 + -0.0 1,630,663 + +0.2 1,630,664 - -0.1 1,630,664 - -0.6 1,630,664 - -0.3 1,630,664 - -2.7 1,630,664 - +0.3 1,630,664 - +0.3
Or see this region's nucleotide sequence