Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27640 and RR42_RS27645 are separated by 89 nucleotides RR42_RS27645 and RR42_RS27650 are separated by 75 nucleotides RR42_RS27650 and RR42_RS27655 are separated by 297 nucleotides
RR42_RS27640: RR42_RS27640 - CDP-diacylglycerol pyrophosphatase, at 1,565,000 to 1,565,776
_RS27640
RR42_RS27645: RR42_RS27645 - hypothetical protein, at 1,565,866 to 1,566,120
_RS27645
RR42_RS27650: RR42_RS27650 - phosphate-binding protein, at 1,566,196 to 1,567,179
_RS27650
RR42_RS27655: RR42_RS27655 - hypothetical protein, at 1,567,477 to 1,568,295
_RS27655
Position (kb)
1566
1567
1568 Strain fitness (log2 ratio)
-1
0
1 at 1565.395 kb on + strand, within RR42_RS27640 at 1565.455 kb on + strand, within RR42_RS27640 at 1565.499 kb on + strand, within RR42_RS27640 at 1565.499 kb on + strand, within RR42_RS27640 at 1565.618 kb on + strand, within RR42_RS27640 at 1565.618 kb on + strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.775 kb on - strand at 1565.827 kb on + strand at 1565.876 kb on - strand at 1565.876 kb on - strand at 1566.053 kb on - strand, within RR42_RS27645 at 1566.067 kb on + strand, within RR42_RS27645 at 1566.068 kb on - strand, within RR42_RS27645 at 1566.260 kb on + strand at 1566.261 kb on - strand at 1566.425 kb on + strand, within RR42_RS27650 at 1566.426 kb on - strand, within RR42_RS27650 at 1566.551 kb on + strand, within RR42_RS27650 at 1566.551 kb on + strand, within RR42_RS27650 at 1566.566 kb on + strand, within RR42_RS27650 at 1566.567 kb on - strand, within RR42_RS27650 at 1566.596 kb on + strand, within RR42_RS27650 at 1566.596 kb on + strand, within RR42_RS27650 at 1566.597 kb on - strand, within RR42_RS27650 at 1566.701 kb on + strand, within RR42_RS27650 at 1566.833 kb on + strand, within RR42_RS27650 at 1566.834 kb on - strand, within RR42_RS27650 at 1566.834 kb on - strand, within RR42_RS27650 at 1566.834 kb on - strand, within RR42_RS27650 at 1566.838 kb on - strand, within RR42_RS27650 at 1567.046 kb on + strand, within RR42_RS27650 at 1567.047 kb on - strand, within RR42_RS27650 at 1567.074 kb on - strand, within RR42_RS27650 at 1567.074 kb on - strand, within RR42_RS27650 at 1567.076 kb on + strand, within RR42_RS27650 at 1567.076 kb on + strand, within RR42_RS27650 at 1567.076 kb on + strand, within RR42_RS27650 at 1567.077 kb on - strand, within RR42_RS27650 at 1567.077 kb on - strand, within RR42_RS27650 at 1567.106 kb on + strand at 1567.106 kb on + strand at 1567.287 kb on - strand at 1567.304 kb on - strand at 1567.304 kb on - strand at 1567.304 kb on - strand at 1567.312 kb on + strand at 1567.312 kb on + strand at 1567.312 kb on + strand at 1567.353 kb on + strand at 1567.354 kb on - strand at 1567.359 kb on - strand at 1567.478 kb on + strand at 1567.652 kb on + strand, within RR42_RS27655 at 1568.072 kb on + strand, within RR42_RS27655 at 1568.073 kb on - strand, within RR42_RS27655 at 1568.073 kb on - strand, within RR42_RS27655 at 1568.142 kb on - strand, within RR42_RS27655
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 1,565,395 + RR42_RS27640 0.51 -1.1 1,565,455 + RR42_RS27640 0.59 +0.2 1,565,499 + RR42_RS27640 0.64 -0.1 1,565,499 + RR42_RS27640 0.64 +0.3 1,565,618 + RR42_RS27640 0.80 +1.1 1,565,618 + RR42_RS27640 0.80 +0.6 1,565,619 - RR42_RS27640 0.80 +0.5 1,565,619 - RR42_RS27640 0.80 -0.6 1,565,619 - RR42_RS27640 0.80 -0.2 1,565,619 - RR42_RS27640 0.80 -0.4 1,565,619 - RR42_RS27640 0.80 +0.1 1,565,775 - -1.3 1,565,827 + +0.6 1,565,876 - +0.5 1,565,876 - +0.5 1,566,053 - RR42_RS27645 0.73 +0.0 1,566,067 + RR42_RS27645 0.79 -0.6 1,566,068 - RR42_RS27645 0.79 -0.2 1,566,260 + -0.9 1,566,261 - +0.1 1,566,425 + RR42_RS27650 0.23 -0.3 1,566,426 - RR42_RS27650 0.23 -0.5 1,566,551 + RR42_RS27650 0.36 +0.7 1,566,551 + RR42_RS27650 0.36 +0.6 1,566,566 + RR42_RS27650 0.38 -0.6 1,566,567 - RR42_RS27650 0.38 -0.4 1,566,596 + RR42_RS27650 0.41 -0.7 1,566,596 + RR42_RS27650 0.41 -0.3 1,566,597 - RR42_RS27650 0.41 -0.1 1,566,701 + RR42_RS27650 0.51 +0.6 1,566,833 + RR42_RS27650 0.65 -1.0 1,566,834 - RR42_RS27650 0.65 +0.4 1,566,834 - RR42_RS27650 0.65 -0.2 1,566,834 - RR42_RS27650 0.65 -0.5 1,566,838 - RR42_RS27650 0.65 -0.3 1,567,046 + RR42_RS27650 0.86 -0.1 1,567,047 - RR42_RS27650 0.86 -0.3 1,567,074 - RR42_RS27650 0.89 -0.0 1,567,074 - RR42_RS27650 0.89 -0.0 1,567,076 + RR42_RS27650 0.89 +0.2 1,567,076 + RR42_RS27650 0.89 -1.2 1,567,076 + RR42_RS27650 0.89 +0.1 1,567,077 - RR42_RS27650 0.90 -0.7 1,567,077 - RR42_RS27650 0.90 +0.4 1,567,106 + -0.6 1,567,106 + -0.4 1,567,287 - -0.0 1,567,304 - +0.9 1,567,304 - -0.3 1,567,304 - -0.1 1,567,312 + +0.3 1,567,312 + +0.1 1,567,312 + -1.4 1,567,353 + -0.1 1,567,354 - -0.0 1,567,359 - -0.7 1,567,478 + -0.8 1,567,652 + RR42_RS27655 0.21 -0.1 1,568,072 + RR42_RS27655 0.73 -0.8 1,568,073 - RR42_RS27655 0.73 -0.5 1,568,073 - RR42_RS27655 0.73 -0.3 1,568,142 - RR42_RS27655 0.81 +0.9
Or see this region's nucleotide sequence