Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23075 and RR42_RS23080 are separated by 204 nucleotides RR42_RS23080 and RR42_RS23085 are separated by 159 nucleotides
RR42_RS23075: RR42_RS23075 - ABC transporter substrate-binding protein, at 511,486 to 512,451
_RS23075
RR42_RS23080: RR42_RS23080 - LysR family transcriptional regulator, at 512,656 to 513,591
_RS23080
RR42_RS23085: RR42_RS23085 - cytochrome C biogenesis protein CcdA, at 513,751 to 514,677
_RS23085
Position (kb)
512
513
514 Strain fitness (log2 ratio)
-2
-1
0
1 at 511.694 kb on + strand, within RR42_RS23075 at 511.695 kb on - strand, within RR42_RS23075 at 511.821 kb on + strand, within RR42_RS23075 at 511.821 kb on + strand, within RR42_RS23075 at 511.822 kb on - strand, within RR42_RS23075 at 511.907 kb on + strand, within RR42_RS23075 at 511.908 kb on - strand, within RR42_RS23075 at 511.908 kb on - strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.992 kb on + strand, within RR42_RS23075 at 511.993 kb on - strand, within RR42_RS23075 at 512.221 kb on - strand, within RR42_RS23075 at 512.235 kb on + strand, within RR42_RS23075 at 512.235 kb on + strand, within RR42_RS23075 at 512.236 kb on - strand, within RR42_RS23075 at 512.236 kb on - strand, within RR42_RS23075 at 512.236 kb on - strand, within RR42_RS23075 at 512.385 kb on - strand at 512.452 kb on + strand at 512.452 kb on + strand at 512.452 kb on + strand at 512.470 kb on + strand at 512.526 kb on + strand at 512.535 kb on + strand at 512.535 kb on + strand at 512.535 kb on + strand at 512.535 kb on + strand at 512.536 kb on - strand at 512.536 kb on - strand at 512.536 kb on - strand at 512.536 kb on - strand at 512.536 kb on - strand at 512.537 kb on + strand at 512.537 kb on + strand at 512.836 kb on + strand, within RR42_RS23080 at 512.837 kb on - strand, within RR42_RS23080 at 513.083 kb on + strand, within RR42_RS23080 at 513.083 kb on + strand, within RR42_RS23080 at 513.084 kb on - strand, within RR42_RS23080 at 513.090 kb on - strand, within RR42_RS23080 at 513.591 kb on + strand at 513.592 kb on - strand at 513.676 kb on - strand at 513.677 kb on + strand at 513.677 kb on + strand at 513.677 kb on + strand at 513.677 kb on + strand at 513.678 kb on - strand at 513.678 kb on - strand at 513.701 kb on + strand at 513.701 kb on + strand at 513.701 kb on + strand at 513.702 kb on - strand at 513.760 kb on - strand at 513.773 kb on - strand at 513.773 kb on - strand at 513.783 kb on - strand at 514.123 kb on + strand, within RR42_RS23085 at 514.123 kb on + strand, within RR42_RS23085 at 514.124 kb on - strand, within RR42_RS23085 at 514.124 kb on - strand, within RR42_RS23085 at 514.124 kb on - strand, within RR42_RS23085 at 514.158 kb on + strand, within RR42_RS23085 at 514.253 kb on - strand, within RR42_RS23085 at 514.284 kb on - strand, within RR42_RS23085 at 514.478 kb on - strand, within RR42_RS23085 at 514.478 kb on - strand, within RR42_RS23085 at 514.504 kb on + strand, within RR42_RS23085 at 514.504 kb on + strand, within RR42_RS23085 at 514.504 kb on + strand, within RR42_RS23085 at 514.505 kb on - strand, within RR42_RS23085 at 514.505 kb on - strand, within RR42_RS23085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 511,694 + RR42_RS23075 0.22 +0.3 511,695 - RR42_RS23075 0.22 +0.9 511,821 + RR42_RS23075 0.35 +0.8 511,821 + RR42_RS23075 0.35 -0.0 511,822 - RR42_RS23075 0.35 +0.1 511,907 + RR42_RS23075 0.44 +0.1 511,908 - RR42_RS23075 0.44 +0.0 511,908 - RR42_RS23075 0.44 -0.7 511,992 + RR42_RS23075 0.52 +0.4 511,992 + RR42_RS23075 0.52 -0.5 511,992 + RR42_RS23075 0.52 -2.2 511,992 + RR42_RS23075 0.52 -0.4 511,993 - RR42_RS23075 0.52 -0.3 512,221 - RR42_RS23075 0.76 +1.7 512,235 + RR42_RS23075 0.78 +0.5 512,235 + RR42_RS23075 0.78 +1.3 512,236 - RR42_RS23075 0.78 +0.5 512,236 - RR42_RS23075 0.78 +0.2 512,236 - RR42_RS23075 0.78 +0.3 512,385 - -0.6 512,452 + -0.3 512,452 + -0.8 512,452 + -1.7 512,470 + +0.1 512,526 + -0.1 512,535 + +0.5 512,535 + +0.1 512,535 + +0.6 512,535 + +0.5 512,536 - +0.2 512,536 - +0.5 512,536 - +0.3 512,536 - +0.1 512,536 - -0.7 512,537 + -1.7 512,537 + +0.3 512,836 + RR42_RS23080 0.19 +0.2 512,837 - RR42_RS23080 0.19 -0.8 513,083 + RR42_RS23080 0.46 +0.2 513,083 + RR42_RS23080 0.46 -0.6 513,084 - RR42_RS23080 0.46 -0.2 513,090 - RR42_RS23080 0.46 -0.1 513,591 + +0.8 513,592 - -0.2 513,676 - -0.6 513,677 + -0.2 513,677 + -0.1 513,677 + -0.7 513,677 + +0.6 513,678 - -0.2 513,678 - -0.7 513,701 + -0.1 513,701 + +0.5 513,701 + +0.7 513,702 - +0.1 513,760 - -0.7 513,773 - -0.4 513,773 - +1.3 513,783 - -0.2 514,123 + RR42_RS23085 0.40 +1.1 514,123 + RR42_RS23085 0.40 -0.2 514,124 - RR42_RS23085 0.40 -0.5 514,124 - RR42_RS23085 0.40 +0.6 514,124 - RR42_RS23085 0.40 +0.1 514,158 + RR42_RS23085 0.44 -1.3 514,253 - RR42_RS23085 0.54 -0.2 514,284 - RR42_RS23085 0.57 -0.8 514,478 - RR42_RS23085 0.78 -0.5 514,478 - RR42_RS23085 0.78 -0.5 514,504 + RR42_RS23085 0.81 -0.3 514,504 + RR42_RS23085 0.81 +0.5 514,504 + RR42_RS23085 0.81 +0.6 514,505 - RR42_RS23085 0.81 -0.0 514,505 - RR42_RS23085 0.81 -0.9
Or see this region's nucleotide sequence